Artículos de revistas
Nanocytotoxicity: Violacein And Violacein-loaded Poly (d, L-lactide-co-glycolide) Nanoparticles Acting On Human Leukemic Cells
Registro en:
Journal Of Biomedical Nanotechnology. , v. 5, n. 2, p. 192 - 201, 2009.
15507033
10.1166/jbn.2009.1018
2-s2.0-67649386621
Autor
Melo P.S.
De Azevedo M.M.M.
Frungillo L.
Anazetti M.C.
Marcato P.D.
Duran N.
Institución
Resumen
Violacein is a compound obtained from Chromobacterium violaceum, a bacterium found in the Amazonian region. Violacein-loaded poly (D, L-lactide-co-glycolide) nanoparticles has a similar inhibitory effect evaluated by trypan blue assay on leukemic HL60 cells than the free form. However, the cytotoxic effects evaluated by phosphatase activity and MTT reduction assays were lower for the encapsulated form than for free violacein. Based on morphological changes, violacein and violacein entrapped in nanoparticles were found to induce terminal differentiation (assessed by nitro blue tetrazolium reduction) in HL60 cells. Thus, both formulations inhibit HL60 cell growth in vitro, partly by inducing cytotoxic effects and cell differentiation. Flow cytometric analysis of HL60 cells after treatment for 12 h showed that violacein-loaded PLGA induced apoptosis, with maximum cell death at a concentration of 2 μM. Violacein and violacein/PLGA induced opposite effects on the mitochondrial swelling which indicates altered mitochondrial function. The mitochondrial activity was also checked by flow cytometry studies. Labelled cells with the probe JC1 displayed a basal hypopolarized status of the mitochondria in treated cells. Based on morphological changes, alterations in phospholipid asymmetry and changes in mitochondrial polarization, violacein and nanoparticles containing violacein were found to trigger cell death by apoptosis. These methodologies are promising as diagnostic and mechanistic effects of nanoparticles in cell cultures. Copyright © 2009 American Scientific Publishers All rights reserved. 5 2 192 201 Durán, N., Menck, C.F.M., Chromobacterium violaceum: A review of pharmacological and industrial perspectives (2001) Crit. Rev. Microbiol., 27, p. 201 Melo, P.S., Justo, G.Z., De Azevedo, M.B.M., Durán, N., Haun, M., Violacein and its complexes induce apoptosis and differentiation in HL60 cells (2003) Toxicology, 186, p. 217 Dessaux, Y., Elmerich, C., Faure, D., Violacein: A molecule of biological interest originating from the soil-borne bacterium Chromobacterium violaceum (2004) La Revue de Médecine Interne, 25, p. 659 Saraiva, V.S., Marshall, J.C., Cools-Lartigue, J., Burnier, M.N., Cytotoxic effects of violacein in human uveal melanoma cell lines (2004) Melanoma Res., 14, p. 421 Durán, N., Justo, G.Z., Ferreira, C.V., Melo, P.S., Cordi, L., Martins, D., Violacein: Properties and biological activities (2007) Biotechnol. Appl. Biochem., 48, p. 127 Shenoy, D.B., Amiji, M.M., Poly(ethylene oxide)-modified poly(ε-caprolactone) nanoparticles for targeted delivery of tamoxifen in breast cancer (2005) Int. J. Pharm., 293, p. 261 Rao, G.C., Kumar, M.S., Mathivanan, N., Rao, M.E., Nanosuspensions at the most promising approach in nanoparticulate drug delivery systems (2004) Pharmazie, 59, p. 5 Jin, S., Ye, K.M., Nanoparticle-mediated drug delivery and gene therapy (2007) Biotechnol. Progr., 23, p. 32 Kabanov, A.V., Batrakova, E.V., Miller, D.W., Pluronic block copolymers as modulators of drug efflux transporter activity in the blood-brain barrier (2003) Advan. Drug Deliv. Rev., 55, p. 151 Minko, T., Batrakova, E.V., Li, S., Li, Y.L., Pakunlu, T.I., Alakhov, V.Y., Kabanov, A.V., Pluronic block copolymers alter apoptotic signal transduction of doxorubicin in drug-resistant cancer cells (2005) J. Control. Release, 105, p. 269 Rapoport, N., Marin, A.P., Timoshin, A.A., Effect of a polymeric surfactant on electron transport in HL60 cells (2000) Arch. Biochem. Biophys., 384, p. 100 Kohli, E., Han, H.Y., Zeman, A.D., Vinogradov, S.V., Formulations of biodegradable Nanogel carriers with 5′- triphosphates of nucleoside analogs that display a reduced cytotoxicity and enhanced drug activity (2007) J. Control. Release, 121, p. 19 Uzunoglu, S., Uslu, R., Tobu, M., Saydam, G., Terzioglu, E., Buyukkececi, F., Omay, S.B., Augmentation of methylprednisolone-induced differentiation of myeloid leukemia cells by serine/threonine protein phosphatase inhibitors (1999) Leukaemia Res., 23, p. 507 Anazetti, M.C., Melo, P.S., Durán, N., Haun, M., Comparative cytotoxicity of dimethylamide-crotonin in the promyelocytic leukemia cell line (HL60) and human peripheral blood mononuclear cells (2003) Toxicology, 188, p. 261 Redmond, K.M., Wilson, T.R., Johnston, P.G., Longley, D.B., Resistance mechanisms to cancer chemotherapy (2008) Frontiers Biosci., 13, p. 5138 Melet, A., Song, K., Bucur, O., Jagani, Z., Grassian, A.R., Khosravi-Far, R., Apoptotic pathways in tumor progression and therapy (2008) Advan. Exper. Med. Biol., 615, p. 47 Debatin, K.M., Activation of apoptosis pathways by anticancer treatment (2000) Toxicol. Lett., 112, p. 41 Inayat-Hussain, S.H., Osman, A.B., Din, L.B., Taniguchi, N., Altholactone, a novel styryl-lactone induces apoptosis via oxidative stress in human HL60 leukemia cells (2002) Toxicol. Lett., 131, p. 153 Kim, R., Emi, M., Tanabe, K., The role of apoptosis in câncer cell survival and therapeutic outcome (2006) Cancer Biol. Ther., 30, p. 5 Anuradha, C.D., Kanno, S., Hirano, S., Oxidative damage to mitochondria is a preliminary step to caspase-3 activation in fluoride-induced apoptosis in HL60 cells (2001) Free Radic. Biol. Med., 31, p. 367 Stoetzer, O.J., Progrebniak, A., Pelka-Fleischer, R., Hasmann, M., Hiddemann, W., Nuessler, V., Modulation of apoptosis by mitochondrial uncouplers: Apoptosis-delying features despite intrinsic cytotoxicity (2002) Biochem. Pharmacol., 63, p. 471 Ujihara, M., Nomura, K., Yamada, O., Deura, H., Establishment of a stable HL60 subline having the potential for monocytic differentiation using granulocyte-macrophage colony-stimulating factor: Possible use for the study of monocytic differentiation and oxidative stress (1998) Atherosclerosis, 139, p. 301 Sokoloski, J.A., Sartorelli, A.C., Induction of the differentiation of HL60 promyelocytic leukemia cells by nonsteroidal anti-inflammatory agents in combination with low levels of vitamin D 3 (1997) Leukemia. Res., 22, p. 153 Wyllie, A.H., Kerr, J.F.K., Currie, A.R., Cell Death: The significance of apoptosis (1980) Int. Rev. Cytol., 68, p. 251 Israels, L.G., Israels, E.D., Apoptosis (1999) Steam Cells, 17, p. 306 Kohroki, J., Norio, M.A., Tanaka, T., Itoh, N., Inada, A., Tanaka, K., Induction of differentiation and apoptosis by dithizone in human myeloid leukemia cell lines (1998) Leukemia Res., 22, p. 405 Minko, T., Kopecková, P., Kopecek, J., Preliminary evaluation of caspases-dependent apoptosis signaling pathways of free and HPMA copolymer-bound doxorubicin in human ovarian carcinoma cells (2001) J. Control. Release, 71, p. 227 Minko, T., Pakunlu, R.I., Wang, Y., Khandar, J.J., Saad, M., New generation of liposomal drugs for cancer (2006) Anticancer Agents Med. Chem., 6, p. 537 Kodach, L.L., Bos, C.L., Durán, N., Peppelenbosch, M.P., Ferreira, C.V., Hardwick, J.C., Violacein synegistically increases 5-fluorouracil cytotoxicity, induces apoptosis and inhibits Akt-mediated signal transduction in humancolorectal cancer cells (2006) Carcinogenesis, 27, p. 508 De Carvalho, D.D., Costa, F.T.M., Durán, N., Haun, M., Cytotoxic activity of violacein in human colon cancer cells (2006) Toxicol. in Vitro, 20, p. 1514 Rettori, D., Durán, N., Production, extraction and purification of violacein: An antibiotic produced by Chromobacterium violaceum (1998) World J. Microbiol. Biotechnol., 14, p. 685 Ferdous, A.J., Stembridge, N.Y., Singh, M., Role of monensin PLGA polymer nanoparticles and liposomes as potentiator of ricin a immunotoxins in vitro (1998) J. Control. Release, 50, p. 71 Denizot, F., Lang, R., Rapid colorimetric assay for cell growth and survival, modifications to the tetrazolium dye procedure giving improved sensitivity and reliability (1986) J. Immunol. Methods, 89, p. 271 Schneider, W.C., Hogeboom, G.H., Intracellular distribution of enzymes. V. Further studies of cytochrome c in rat liver homogenates (1950) J. Biol. Chem., 183, p. 123 Anazetti, M.C., Melo, P.S., Durán, N., Haun, M., Dehydrocrotonin and its derivative, dimethylamide-crotonin induce apoptosis with lipid peroxidation and activation of caspases-2, -6 and -9 in human leukemic cells HL60 (2004) Toxicology, 203, p. 123 Araragi, S., Kondoh, M., Kawase, M., Saito, S., Higashimoto, M., Sato, M., Mercuric chloride induces apoptosis via a mitochondrial-dependent pathway in human leukemia cells (2003) Toxicology, 184, p. 1 Fillon, S., Lang, F., Jendrossek, V., Pseudomonas aeruginosa triggered apoptosis of human epithelial cells depends on the temperature during infection (2002) Cell Physiol. Biochem., 12, p. 207 Melo, P.S., Maria, S.S., Vidal, B.C., Haun, M., Durán, N., Violacein cytotoxicity and induction of apoptosis in V79 cells (2000) In Vitro Cell. Develop. Biol.-Animal, 36, p. 539 Pailard, F., Finot, F., Mouche, I., Prenez, A., Vericat, J.A., Use of primary cultures of rat hepatocytes to predict toxicity in the early development of new chemical entities (1999) Toxicol. in Vitro, 13, p. 693 Mosmann, T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay (1983) J. Immunol. Methods, 65, p. 55 Freire, A.C.G., Aoyama, H., Haun, M., Ferreira, C.V., Relationship between phosphatase inhibitors, okadaic acid and pervanadate, on human myeloid leukaemia cell line (2003) J. Enzyme Inhib. Med. Chem., 18, p. 425 Mehar, A., Macanas-Pirard, P., Mizokami, A., Takahashi, Y., Kass, G.E.N., Coley, H.M., The effects of cyclooxygenase-2 expression in prostate cancer cells: Modulation of response to cytotoxic agents (2008) J. Pharmacol. Exper. Ther., 324, p. 1181 Rossi, D., Gaidano, G., Messengers of cell death: Apoptotic signalling in health and disease (2003) Haematologica, 88, p. 212 Lee, J.Y., Hwang, W.I., Lim, S.T., Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. de Candolle roots (2004) J. Ethnopharmacol., 93, p. 409 Facompré, M., Wattez, N., Kluza, J., Lansiaux, A., Bailly, C., Relationship between cell cycle changes and variations of the mitochondrial membrane potential induced by etoposide (2000) Mol. Cell Biol. Re. Comm., 4, p. 37 Greenebaum, B., Blossfield, K., Hannig, J., Carrillo, C.S., Beckett, M.A., Weichselbaum, R.R., Lee, R.C., Poloxamer 188 prevents acute necrosis of adult skeletal muscle cells following high-dose irradiation (2004) Burns, 30, p. 539 Luo, J., Borgens, R., Shi, R., Polyethylene glycol immediately repairs neuronal membranes and inhibits free radical production after acute spinal cord injury (2002) J. Neurochem., 83, p. 471 Marks, J.D., Pan, C., Bushell, R., Cromie, W., Lee, R.C., Amphiphilic, tri-block copolymers provide potent, membrane-targeted neuroprotection (2001) FASEB J., 15, p. 1107 Maskarinec, S.A., Lee, K.Y.C., Comparative study of poloxamer insertion into lipid monolayers (1809) Langmuir, 19, p. 2003 Tharmalingam, T., Ghebeth, H., Wuerz, T., Butler, M., Pluronic enhances the robustness and reduces the cell attachment of mammalian cells (2008) Mol. Biotechnol., 39, p. 167