Artículos de revistas
Glucosyltransferase Production By Klebsiella Sp. K18 And Conversion Of Sucrose To Palatinose Using Immobilized Cells
Registro en:
Brazilian Journal Of Microbiology. , v. 40, n. 1, p. 66 - 72, 2009.
15178382
2-s2.0-67149111912
Autor
Orsi D.C.
Kawaguti H.Y.
Sato H.H.
Institución
Resumen
The strain Klebsiella sp. K18 produces the enzyme glucosyltransferase and catalyses the conversion of sucrose to palatinose, an alternative sugar that presents low cariogenicity. Response Surface Methodology was successfully employed to determine the optimal concentration of culture medium components. Maximum glucosyltransferase production (21.78 U mL-1) was achieved using the optimized medium composed by sugar cane molasses (80 g L-1), bacteriological peptone (7 g L-1) and yeast extract (20 g L -1), after 8 hours of fermentation at 28°C. The conversion of sucrose to palatinose was studied utilizing immobilized cells in calcium alginate. The effects of the alginate concentration (2-4%), cell mass concentration (20-40%) and substrate concentration (25-45%) were evaluated and the yield of palatinose was approximately 62.5%. 40 1 66 72 Cummings, J.H., MacFarlane, G.T.E., Englyst, H.N., Prebiotic digestion and fermentation (2001) American J. Clin, 73, pp. 415-420 Heikkila, H., Sarkki, M., Lindroos, M., Ojala, P., Ravanko, V., Tylli, M., Process of the production of isomaltulose and other products (2000), U.S. Pat. 6, 146, 856Huang, J.H., Hsu, L.H., Su, Y.C., Conversion of sucrose to isomaltulose by Klebsiella planticola CCRC 19112 (1998) J. Ind. Microbiol. Biotechnol, 21, pp. 22-27 Kawaguti, Y.H., Manrich, E., Sato, H.H., Production of isomaltulose using Erwinia sp D12 cells: Culture medium optimization and cell immobilization in alginate (2006) Biochem. Eng. J, 29, pp. 270-277 Kawaguti, Y.H., Manrich, E., Fleuri, F.L., Sato, H.H., Production of glucosyltransferase by Erwinia sp using experimental design and response surface methodology (2005) Braz. J. Microbiol, 36, pp. 227-234 Li, X., Zhao, C., An, Q., Zhang, D., Substrate induction of isomaltulose synthase in a newly isolated Klebsiella sp. LX3 (2003) J. Appl. Microbiol, 95, pp. 521-527 Lina, B.A.R., Jonker, D., Kozianowski, G., Isomaltulose (Palatinose): A review of biological and toxicological studies (2002) Food Chem. Toxicol, 40, pp. 1375-1381 Minami, T., Fujiwara, T., Ooshima, T., Nakajima, Y., Hamada, S., Interaction of structural isomers of sucrose in the reaction between sucrose and glucosyltransferases from mutans Streptococci (1990) Oral Microbiol. Immunol, 5, pp. 189-194 Moraes, A.L.L., Steckelberg, C., Sato, H.H., Pinheiro, A., Produção de isomaltulose a partir da transformação enzimática da sacarose, utilizando-se Erwinia sp D12 imobilizada com alginato de cálcio. (2005) Ciênc. Tecnol. Aliment, 25, pp. 95-102 Moreira, R.F., Maria, C.A., Glicídios no mel. (2001) Quím. Nova, 24, pp. 516-525 Park, Y.K., Uekane, R.T., Sato, H.H., Biochemical characterization of a microbial glucosyltransferase that converts sucrose to palatinose (1996) Rev. Microbiol, 27, pp. 131-136 Somogy, M., A new reagent for the determination of sugars (1945) J. Biol. Chem, 160, pp. 61-68 Takazoe, I. (1989). Palatinose-an isomeric alternative to sucrose, In: Elsevier Applied Science (ed). Progress in Sweeteners. London, England, p. 143-167Tsuyuki, K., Sugitani, Y., Miyata, Y., Ebashi, T., Nakajima, Y., Isolation and characterization of trealulose-producing bacteria from Thailand soil (1992) J. Gen. Appl. Microbiol, 38, pp. 483-490 Veronese, T., Perlot, P., Mechanism of sucrose conversion by the sucrose isomerase of Serratia plymuthica ATCC 15928 (1999) Enzyme Microb. Technol, 24, pp. 263-269 Ziesenitz, C.S. Sibert, G. (1987). The metabolism and utilization of polyois and other bulk sweeteners compared with sugar. In: Elsevier Applied Science (ed). Developments in sweeteners. London, England, p. 109-149