| dc.creator | Papadimitriou A. | |
| dc.creator | Peixoto E.B.M.I. | |
| dc.creator | Silva K.C. | |
| dc.creator | Lopes De Faria J.M. | |
| dc.creator | Lopes De Faria J.B. | |
| dc.date | 2014 | |
| dc.date | 2015-06-25T17:50:47Z | |
| dc.date | 2015-11-26T15:37:22Z | |
| dc.date | 2015-06-25T17:50:47Z | |
| dc.date | 2015-11-26T15:37:22Z | |
| dc.date.accessioned | 2018-03-28T22:45:47Z | |
| dc.date.available | 2018-03-28T22:45:47Z | |
| dc.identifier | | |
| dc.identifier | Cellular Physiology And Biochemistry. S. Karger Ag, v. 34, n. 4, p. 1260 - 1272, 2014. | |
| dc.identifier | 10158987 | |
| dc.identifier | 10.1159/000366336 | |
| dc.identifier | http://www.scopus.com/inward/record.url?eid=2-s2.0-84908615597&partnerID=40&md5=ea5b39c3cb1c55bae53f516cbacb4227 | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/85915 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/85915 | |
| dc.identifier | 2-s2.0-84908615597 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1263628 | |
| dc.description | Background/Aims: High phosphate (Pi) levels and extracellular matrix (ECM) accumulation are associated with chronic kidney disease progression. However, how high Pi levels contribute to ECM accumulation in mesangial cells is unknown. The present study investigated the role and mechanism of high Pi levels in ECM accumulation in immortalized human mesangial cells (iHMCs). Methods: iHMCs were exposed to normal (0.9 mM) or increasing Pi concentrations (2.5 and 5 mM) with or without diferent blockers or activators. NOX4, phosphorylated AMPK (p-AMPK), phosphorylated SMAD3 (p-SMAD3), fbronectin (F/N), collagen IV (C-IV) and alpha-smooth muscle actin (α-SMA) expression was assessed via western blot and immunofuorescence. Lucigenin-enhanced chemiluminescence, and dihydroethidium (DHE) assessed NADPH oxidase activity and superoxide (SO), respectively. Results: In iHMCs, a Pi transporter blocker (PFA) abrogated high Pi-induced AMPK inactivation, increase in NADPH oxidase-induced reactive oxygen species (ROS) levels, NOX4, p-SMAD3, α-SMA and C-IV expression. AMPK activation by AICAR, NOX4 silencing or NADPH oxidase blocker prevented high Pi-induced DHE levels, p-SMAD3, F/N, C-IV and α-SMA expression. Conclusion: AMPK inactivation with NOX4-induced ROS formation and transforming growth factor β-1 (TGFβ-1) signaling activation mediates high Pi-induced ECM accumulation in iHMCs. Maneuvers increasing AMPK or reducing NOX4 activity may contribute to renal protection under hyperphosphatemia. | |
| dc.description | 34 | |
| dc.description | 4 | |
| dc.description | 1260 | |
| dc.description | 1272 | |
| dc.description | Voormolen, N., Noordzij, M., Grootendorst, D.C., Beetz, I., Sijpkens, Y.W., Van Manen, J.G., Boeschoten, E.W., Dekker, F.W., High plasma phosphate as a risk factor for decline in renal function and mortality in pre-dialysis patients (2007) Nephrol Dial Transplant, 22, pp. 2909-2916 | |
| dc.description | Kestenbaum, B., Phosphate metabolism in the setting of chronic kidney disease: Significance and recommendations for treatment (2007) Semin Dial, 20, pp. 286-294 | |
| dc.description | Zoccali, C., Ruggenenti, P., Perna, A., Leonardis, D., Tripepi, R., Tripepi, G., Mallamaci, F., Remuzzi, G., Phosphate may promote ckd progression and attenuate renoprotective effect of ace inhibition (2011) J Am Soc Nephrol, 10, pp. 1923-1930 | |
| dc.description | Finch, J.L., Lee, D.H., Liapis, H., Ritter, C., Zhang, S., Suarez, E., Ferder, L., Slatopolsky, E., Phosphate restriction significantly reduces mortality in uremic rats with established vascular calcification (2013) Kidney Int, 84, pp. 1145-1153 | |
| dc.description | Eräranta, A., Riutta, A., Fan, M., Koskela, J., Tikkanen, I., Lakkisto, P., Niemelä, O., Pörsti, I., Dietary phosphate binding and loading alter kidney angiotensin-converting enzyme mrna and protein content in 5/6 nephrectomized rats (2012) Am J Nephrol, 35, pp. 401-408 | |
| dc.description | Chen, Z., Chen, D., McCarthy, T.L., Centrella, M., Zhang, Y., Moeckel, G.W., Inorganic phosphate stimulates fibronectin expression in renal fibroblasts (2012) Cell Physiol Biochem, 30, pp. 151-159 | |
| dc.description | Chatziantoniou, C., Dussaule, J.C., Insights into the mechanisms of renal fibrosis: Is it possible to achieve regression? (2005) Am J Physiol Renal Physiol, 289, pp. F227-F234 | |
| dc.description | Abboud, H.E., Mesangial cell biology (2012) Exp Cell Res, 318, pp. 979-985 | |
| dc.description | Gorin, Y., Block, K., Hernandez, J., Bhandari, B., Wagner, B., Barnes, J.L., Abboud, H.E., Nox4 nad(p)h oxidase mediates hypertrophy and fibronectin expression in the diabetic kidney (2005) J Biol Chem, 280, pp. 39616-39626 | |
| dc.description | Lee, D.Y., Wauguier, F., Eid, A.A., Roman, L.J., Choudhury, G.G., Khazin, K., Block, K., Gorin, Y., Nox4 nadph oxidase mediates peroxynitrite-dependent uncoupling of endothelial nitric oxide synthase and fibronectin expression in response to angiotensin II. Role of mitochondrial reactive oxygen species (2013) J Biol Chem, 288, pp. 28668-28686 | |
| dc.description | Papadimitriou, A., Peixoto, E.B., Silva, K.C., Lopes De Faria, J.M., Lopes De Faria, J.B., Increase in ampk brought about by cocoa is renoprotective in experimental diabetes mellitus by reducing nox4/tgfb-1 signalling (2014) J Nutr Biochem, 25, pp. 773-784 | |
| dc.description | Long, Y.C., Zierath, J.R., AMP-protein kinase signaling in metabolic regulation (2006) J Clin Invest, 116, pp. 1776-1783 | |
| dc.description | Hallows, K.R., Mount, P.F., Pastor-Soler, N.M., Power, D.A., Role of the energy sensor amp-activated protein kinase in renal physiology and disease (2010) Am J Physiol Renal Physiol, 298, pp. F1067-F1077 | |
| dc.description | Hawley, S.A., Davison, M., Woods, A., Davies, S.P., Beri, R.K., Carling, D., Hardie, D.G., Characterization of the amp-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates amp-activated protein kinase (1996) J Biol Chem, 271, pp. 27879-27887 | |
| dc.description | Hardie, D.G., Ross, F.A., Hawley, S.A., AMP-activated protein kinase: A target for drugs both ancient and modern (2012) Chem Biol, 19, pp. 1222-1236 | |
| dc.description | Declèves, A.E., Sharma, K., Novel targets of antifibrotic and anti-inflammatory treatment in ckd (2014) Nat Rev Nephrol, 10, pp. 257-267 | |
| dc.description | Satriano, J., Sharma, K., Blantz, R.C., Deng, A., Induction of ampk activity corrects early pathophysiological alterations in the subtotal nephrectomy model of chronic kidney disease (2013) Am J Physiol Renal Physiol, 305, pp. F727-F733 | |
| dc.description | Dugan, L.L., You, Y.H., Ali, S.S., Diamond-Stanic, M., Miyamoto, S., Decleves, A.E., Andreyev, A., Sharma, K., AMPK dysregulation promotes diabetes-related reduction of superoxide and mitochondrial function (2013) J Clin Invest, 123, pp. 4888-4899 | |
| dc.description | Lee, M.J., Feliers, D., Mariappan, M.M., Sataranatarajan, K., Mahimainathan, L., Musi, N., Foretz, M., Kasinath, B.S., A role for amp-activated protein kinase in diabetes-induced renal hypertrophy (2007) Am J Physiol Renal Physiol, 292, pp. F617-F627 | |
| dc.description | Block, K., Eid, A., Griendling, K.K., Lee, D.Y., Wittrant, Y., Gorin, Y., Nox4 nad(p)h oxidase mediates src-dependent tyrosine phosphorylation of pdk-1 in response to angiotensin ii: Role in mesangial cell hypertrophy and fibronectin expression (2008) J Biol Chem, 283, pp. 24061-24076 | |
| dc.description | Banas, B., Luckow, B., Möller, M., Klier, C., Nelson, P.J., Schadde, E., Brigl, M., Schlöndorff, D., Chemokine and chemokine receptor expression in a novel human mesangial cell line (1999) J Am Soc Nephrol, 10, pp. 2314-2322 | |
| dc.description | Cristovam, P.C., Arnoni, C.P., De Andrade, M.C., Casarini, D.E., Pereira, L.G., Schor, N., Boim, M.A., ACE-dependent and chymase-dependent angiotensin ii generation in normal and glucose stimulated human mesangial cells (2008) Exp Biol Med (Maywood), 233, pp. 1035-1043 | |
| dc.description | Ribaldo, P.D.B., Souza, D.S., Biswas, S.K., Block, K., Lopes De Faria, J.M., Lopes De Faria, J.B., Green tea (camelia sinensis) attenuates nephropathy by down-regulating nox4 nadph oxidase in diabetic spontaneously hypertensive rats (2009) J Nutr, 139, pp. 96-100 | |
| dc.description | Faria, A.M., Papadimitriou, A., Silva, K.C., Lopes De Faria, J.M., Lopes De Faria, J.B., Uncoupling endothelial nitric oxide synthase is ameliorated by green tea in experimental diabetes by re-establishing tetrahydrobiopterin levels (2012) Diabetes, 61, pp. 1838-1847 | |
| dc.description | Biswas, S.K., Peixoto, E.B., Souza, D.S., Lopes De Faria, J.B., Hypertension increases pro-oxidant and decreases antioxidant defense in the kidney in early diabetes (2008) Am J Nephrol, 28, pp. 133-142 | |
| dc.description | Sedeek, M., Callera, G., Montezano, A., Gutsol, A., Heitz, F., Szyndralewiez, C., Page, P., Hebert, R.L., Critical role of nox4-based nadph oxidase in glucose-induced oxidative stress in the kidney: Implications in type 2 diabetic nephropathy (2010) Am J Physiol Renal Physiol, 299, pp. F1348-F1358 | |
| dc.description | Gorin, Y., Ricono, J.M., Wagner, B., Kim, N.H., Bhandari, B., Choudhury, G.G., Abboud, H.E., Angiotensin ii-induced erk1/erk2 activation and protein synthesis are redox-dependent in glomerular mesangial cells (2004) Biochem J, 381, pp. 231-239 | |
| dc.description | Gorin, Y., Ricono, J.M., Kim, N.H., Bhandari, B., Choudhury, G.G., Abboud, H.E., Nox4 mediates angiotensin ii-induced activation of akt/protein kinase b in mesangial cells (2003) Am J Physiol Renal Physiol, 285, pp. F219-F229 | |
| dc.description | Jha, J.C., Gray, S.P., Barit, D., Okabe, J., El-Osta, A., Namikoshi, T., Thallas-Bonke, V., Jandeleit-Dahm, K.A., Genetic targeting of pharmacological inhibition of nadph oxidase nox4 provides renoprotection in long-term diabetic nephropathy (2014) J Am Soc Nephrol, 25, pp. 1237-1254 | |
| dc.description | Kim, M.Y., Lim, J.H., Youn, H.H., Hong, Y.A., Yang, K.S., Park, H.S., Chung, S., Park, C.W., Resveratrol prevents renal lipotoxicity and inhibits mesangial cell glucotoxicity in a manner dependent on the ampk-sirt1-pgc1a axis in db/db mice (2013) Diabetologia, 56, pp. 204-217 | |
| dc.description | Ding, D.F., You, N., Wu, X.M., Xu, J.R., Hu, A.P., Ye, X.L., Zhu, Q., Lu, Y.B., Resveratrol attenuates renal hypertrophy in early-stage diabetes by activating ampk (2010) Am J Nephrol, 31, pp. 363-374 | |
| dc.language | en | |
| dc.publisher | S. Karger AG | |
| dc.relation | Cellular Physiology and Biochemistry | |
| dc.rights | aberto | |
| dc.source | Scopus | |
| dc.title | Inactivation Of Ampk Mediates High Phosphate-induced Extracellular Matrix Accumulation Via Nox4/tgfβ-1 Signaling In Human Mesangial Cells | |
| dc.type | Artículos de revistas | |
