Artículos de revistas
Expanded Bed Adsorption Of Bromelain (e.c. 3.4.22.33) From Ananas Comosus Crude Extract
Registro en:
Brazilian Journal Of Chemical Engineering. , v. 26, n. 1, p. 149 - 157, 2009.
1046632
2-s2.0-63449139510
Autor
Silveira E.
Souza Jr. M.E.
Santana J.C.C.
Chaves A.C.
Porto A.L.F.
Tambourgi E.B.
Institución
Resumen
This work focuses on the adsorption of Bromelain in expanded bed conditions, such as the adsorption kinetics parameters. The adsorption kinetics parameters showed that after 40 minutes equilibrium was achieved and maximum adsorption capacity was 6.11 U per resin mL. However, the maximum adsorption capacity was only determined by measuring the adsorption isotherm. Only by the Langmuir model the maximum adsorption capacity, Qm, and dissociation constant, kd, values could be estimated as 9.18 U/mL and 0.591, respectively, at 25°C and 0.1 mol/L phosphate buffer pH 7.5. A column made of glass with an inner diameter of 1 cm was used for the expanded bed adsorption (EBA). The residence time was reduced 10 fold by increasing the expansion degree 2.5 times; nonetheless, the plate number (N) value was reduced only 2 fold. After adsorption, the bromelain was eluted in packed bed mode, with a downward flow. The purification factor was about 13 fold and the total protein was reduced 4 fold. EBA showed to be feasible for purification of bromelain. 26 1 149 157 Amersham Pharmacia Biotech. EBA Handbook: Principles and Methods. Uppsala, ISBN 91-630-5519-8 (1997)Anspach, F.B., Curbelo, D., Hartman, R., Garke, G., Deckwer, W.D., Expanded-bed chromatography in primary protein purification (1999) J. Chromatogr. A, 865, p. 129 Bruce, L.J., Chase, H.A., Hydrodynamics and adsorption behaviour within an expanded bed adsorption column studied using in-bed sampling (2001) Chem. Eng. Sci, 56, p. 3149 Camprubi, S., Bruguera, M., Canalias, F., Purification of recombinant histidine-tag streptolysin O using immobilized metal affinity expanded bed adsorption (IMA-EBA). International (2006) J. Biol. Macromolecules, 38, pp. 134-139 Chang, Y.K., McCreath, G.E., Chase, H.A., (1994) Advances in Bioprocess Engineering, p. 519. , Galindo, E, Ramirez, O.T, Eds, Kluwer Academics Publisher: Netherlands, p Chase, H.A., Purification of proteins by adsorption chromatography in expanded beds (1994) Trends Biotechnol, 12, p. 296 Dainiak, M.B., Galaev, I.Y., Matiasson, B., Direct capture of product from fermentation broth using a cell-repelling ion exchanger (2002) J. Chromatogr. A, 942, pp. 123-131 Deutscher, M.P., Guide to protein purification (1990) Method. Enzymol, p. 182 Fernandez-Lahore, H.M., Lin, D.-Q., Hubbuch, J.J., Kula, M.R., Thommes, J., The use of ionselective electrodes for evaluating residence time distributions in expanded bed adsorption systems (2001) Biotechnol. Progr, 17, pp. 1128-1136 Gaspani, L., Limiroli, E., Ferrario, P., Bianchi, M., In vivo and in vitro effects of bromelain on PGE(2) and SP concentrations in the inflammatory exudate in rats (2002) Pharmacology, 65, pp. 83-86 Haq, S.K., Rasheedi, S., Khan, R.H., Characterization of a partially folded intermediate of stem bromelain at low pH (2002) Eur. J. Biochem, 269, pp. 47-52 Harrach, T., Eckert, K., Maurer, H.R., Machleidt, I., Machleidt, W., Nuck, R., Isolation and characterization of two forms of an acidic bromelain stem proteinase (1998) J. Protein Chem, 17, pp. 351-361 Hatano, K., Sawano, Y., Tanokura, M., Structure-function relationship of bromelain isoinhibitors from pineapple stem (2002) Biol. Chem, 383, pp. 1151-1156 Hatano, K., Tanokura, M., Takahashi, K., The amino acid sequences of isoforms of the bromelain inhibitor from pineapple stem (1998) J. Biochem, 124, pp. 457-461 Hjorth, R., Expanded bed adsorption in industrial bioprocessing: Recent developments (1997) Trends Biotechnol, 15, p. 230 Hochstrasser, D.F., Patchornik, A., Merril, C.R., Development of Polyacrylamide gels that improve the separation of proteins and their detection by silver staining (1998) Analyt. Biochem, 173, pp. 412-423 Hochstrasser, D.F., Merril, C.R., Catalysts' for Polyacrylamide gel polymerization and detection of proteins by silver staining (1998) Appl. Theor. Electrophoresis, 1, pp. 35-40 Hebbar, H.U., Sumana, B., Raghavarao, K.S.M.S., Use of reverse micellar systems for the extraction and purification of bromelain term from pineapple wastes (2008) Bioresource Technology, 99, pp. 4896-4902 Khan, R.H., Rasheedi, S., Haq, S.K., Effect of pH, temperature and alcohols on the stability of glycosylated and deglycosylated stem bromelain (2003) J. Biosci, 28, pp. 709-714 Kim, M. H., Kim, H. K., Lee, J. K., Park, S. Y., Oh, T. K., Thermostable lipase of Bacillus stearothermophilus, high level production, purification and calciumdependent thermostability. Korea Res. Inst. Biosci. Biotechnol. 64:280-286 (2000)Kordel, M., Hofmann, B., Schomburg, D., Schimid, R.D., Extracelluar lipase of Pseudomonas sp. strain ATCC 21808: Purification, characterization, crystallization and preliminary X-ray diffraction data (1991) J. Bacteriol, 173, pp. 4836-4841 Kunitz, M., Crystalline soybean trypsin inhibitor: II general properties (1974) J. Gen. Physiol, 30, pp. 291-310 Lali, A.M., Khare, A.S., Joshi, J.B., Behaviour of solid particles in viscous non-newtonian solutions: Settling velocity, wall effects and bed expansion in solid-liquid fluidized beds (1989) Powder Tech, 57, pp. 39-50 Maurer, H.R., Bromelain: Biochemistry, pharmacology and medical use (2001) Cell. Mol. Life Sci, 58, pp. 1234-1245 Mullick, A., Flickinger, M.C., Expanded bed adsorption of human serum albumin from very dense saccharomyces cerevesiae suspensions on fluoride-modified zirconia (1999) Biotechnol. Bioeng, 65, pp. 282-290 Murachi, T., Bromelain enzymes (1976) Methods Enzymol, 45, pp. 475-485 Ota, S., Horie, K., Hagino, F., Hashimoto, C., Date, H., Fractionation and some properties of the proteolytically active components of bromelains in the stem and the fruit of the pineapple plant (1972) J. Biochem, 71, pp. 817-830 Rasheedi, S., Haq, S.K., Khan, R.H., Guanidine hydrochloride denaturation of glycosylated and deglycosylated stem bromelain (2003) Biochemistry, 68, pp. 1097-1100 Roy, I., Pai, A., Lali, A., Gupta, M.N., Comparison of batch, packed bed and expanded bed purification of A. niger cellulose beads (1999) Bioseparation, 8, pp. 317-326 Santos, E.S., Guirardello, R., Franco, T.T., Distributor Effect on Expanded Bed Adsorption (2000) International Conference IEX 2000 (Ion Exchange at the Millennium), , Cambridge/UK Santos, E.S., Guirardello, R., Franco, T.T., Preparative chromatography of xylanase using expanded bed adsorption (2002) J. Chrom. A, 944, pp. 217-224 Silveira, E., (2007) Purificação e caracterização de bromelina a partir do extrato bruto de Ananas comosus por adsorção em leito expandido, , Dissertação de Mestrado. Faculdade de Engenharia Química: Unicamp Silverstein, R.M., Kezdy, F.J., Characterization of the pineapple stem proteases (bromelain) (1975) Arch. Biochem.Biophys, 167, pp. 678-686 Suh, H.J., Lee, H., Cho, H.Y., Yang, H.C., Purification and characterization of bromelain isolated from pineapple (1992) Han'guk Nonghwa Hakhoechi, 35, pp. 300-307 Takahashi, N., Yasuda, Y., Goto, K., Miyake, T., Murachi, T., Multiple molecular forms of stem bromelain. Isolation and characterization of two closely related components, SB1 and SB2 (1973) J. Biochem, 74, pp. 355-373 Tan, Y.P., Ling, T.C., Tan, W.S., Yusoff, K., Tey, B.T., Purification of recombinant nucleocapsid protein of Newcastle disease virus from unclarifled feedstock using expanded bed adsorption chromatography (2006) Prot. Expr. Purif, 46, pp. 114-121 Thömmes, J., Fluidized bed adsorption as a primary recovery step in protein purification (1997) Adv. Biochem. Eng, 58, p. 185 Toledo, A.L., Severo, J.J.B., Souza, R.R., Campos, E.S., Santana, J.C.C., Tambourgi, E.B., Purification by expanded bed adsorption and characterization of an a-amylase FORILASE NTL®from A. niger (2006) J. Chrom. B, 846, pp. 51-56 Trinh, L., Phue, J.-N., Jaluria, P., Tsai, C.W., Narum, D.L., Shiloach, J., Screen-less expanded bed column: New approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris (2006) Biotechnol. Lett, 28, pp. 951-958 Walter, H. E., Proteinases: methods with hemoglobin, casein, and azocoll as substrates. Pp. 270-277 in Methods of Enzymatic Analysis, 5, H.U. Bergmeyer, ed. Verlag Chemie, Weinheim, Germany (1984)Wang, S.-L., Peng, J.-H., Liang, T.-W., Liu, K.-C., Purification and characterization of a chitosanase from Serratia marcescens TKU011 (2008) Carbohydrate Research, 343, pp. 1316-1323 Wharton, C.W., The structure and mechanism of stem bromelain. Evaluation of the homogeneity of purified stem bromelain, determination of the molecular weight and kinetic analysis of the bromelain-catalysed hydrolysis of N-benzyloxycarbonyl-L-phenylalanyl-L-serine methyl ester (1974) Biochem. J, 143, pp. 575-586 Wheelwright, S.M., (1994) Protein purification: Design and scale up of downstream processing, , Munich: Hanser Publishers Yamamoto, S., Okamoto, A., Walter, P., Effects of adsorbent properties on zone spreading in expanded bed chromatography (2001) Bioseparation, 1, pp. 1-10 Yun, J.X., Yao, S.-J., Lin, D.-Q., Lu, M.-H., Zhao, W.-T., Modeling axial distributions of adsorbent particle size and local voidage in expanded bed (2004) Chem. Eng. Sci, 59, p. 449 Yun, X.L., Lin, D.-Q., Lu, M.-H., Zhong, L.-N., Yao, S.-L., Measurement and modeling of axial distribution of adsorbent particles in expanded bed: Taking into account the particle density difference (2004) Chem. Eng. Sci, 59, p. 5873