Endopleura uchi (Huber) Cuatrec. is an Amazon species traditionally used as treatment for inflammations and female disorders. Bergenin was isolated from ethyl acetate fraction of bark of E. uchi by using column chromatography over sephadex LH-20 and then silica gel 60 flash. Its structure was identified on the basis of its NMR spectra. The antimicrobial activity of bergenin and fractions of methanol extract of E. uchi were evaluated against ATCC microorganisms (Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Enterococcus faecalis, Staphylococcus aureus, Candida albicans, C. guilliermondii, Aspergillus flavus, A. nidulans). Clinically isolated strains of all of these microorganisms, along with C. tropicalis, A. niger, Shigella sonnei, Serratia marcenses and Klebsiella pneumoniae were also evaluated. The growth inhibition caused by bergenin, extracts and fractions of E. uchi against ATCC microorganisms were similar to the inhibition to microorganisms clinically isolated. The ethyl acetate fraction and the isolate bergenin inhibit the growth of the yeasts C. albicans, C. tropicalis, and C. guilliermondii, but present lower activity against filamentous fungi Aspergillus flavus, A. nidulans, A. niger, and did not inhibit the Gram positive and Gram negative bacteria. The activity of the ethyl acetate fraction and bergenin are in agreement wit its high concentration found in bark extract of E. uchi. Moreover, the selective activity against three Candida species helps to understand its traditional use against infections that affect women.391187192Ballweg, M.L., Impact of endometriosis on women's health: Comparative historical data show that the earlier the onset, the more severe the disease (2004) Best Practice & Research Clinical Obstetrics and Gynaecology, 18 (2), pp. 201-218Braga, P.C., Culici, M., Alfieri, M., dal Sasso, M., Thymol inhibits Candida albicans biofilm formation and mature biofilm (2008) International Journal of Antimicrobial Agents, 31 (5), pp. 472-477Caldas, C.S., de Simone, C.A., Pereira, M.A., Malta, V.R.S., Carvalho, R.L.P., da Silva, T.B.C., Sant'Ana, A.E.G., Conserva, L.M., Bergenin monohidrate, a constituent of Humiria balsamifera, at 120 K (2002) Acta Chrystallographica, 58, pp. 609-611Corrêa, M.P., (1984) Diccionario das Plantas Úteis do Brasil e das Exóticas Cultivadas, 6, p. 326. , Rio de Janeiro, Imprensa Nacionalda Silva, S.L., Figueiredo, P.M.S., Yano, T., Antibacterial and antifungal activities of volatile oils from Zanthoxylum rhoifolium leaves (2006) Pharmaceutical Biology, 44 (9), pp. 657-659da Silva, S.L., Figueiredo, P.M.S., Yano, T., Antimicrobial activity of ethanol extract from leaves of Casearia sylvestris (Flacourtiaceae) (2008) Pharmaceutical Biology, 46 (5), pp. 347-351Hoffman, H.L., Pfaller, M.A., In vitro antifungal susceptibility testing (2001) Pharmacotherapy, 21 (8), pp. 111-123Hussain, A.I., Anwar, F., Sherazi, S.T.H., Przybylski, R., Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations (2008) Food Chemistry, 108 (3), pp. 986-995Jahodar, I., Kolb, I., Lioka, A., NMR spectral analysis of bergenin (1992) Fitoterapia, 63 (3), pp. 260-261Lim, H.K., Kim, H.S., Choi, H.S., Oh, S., Choi, J., Hepatoprotective effects of bergenin, a major constituent of Mallotus japonicus, on carbon tetrachloride-intoxicated rats (2000) Journal of Ethnopharmacology., 72 (3), pp. 469-474Magalhães, L.A., Lima, M.P., Marinho, H.A., Ferreira, A.G., Identificação de bergenina e carotenóides no fruto de uchi (Endopleura uchi, Humiriaceae) (2007) Acta Amazônica, 37 (3), pp. 447-450Maillard, M., Gupta, M.P., Hostettmann, K., A new antifungal prenylated flavonone from Erythrina herteroana (1987) Planta Medica, 53 (6), pp. 563-564Marx, F., Andrade, E.H.A., Zoghbi, M.G., Maia, J.G.S., Studies of edible Amazonian plants. Part 5: Chemical characterization of Amazonian Endopleura uchi fruits (2002) European Food Research and Technology, 214 (4), pp. 331-334Nazir, N., Koul, S., Qurishi, M.A., Taneja, S.C., Ahmad, S.F., Bani, S., Qazi, G.N., Immunomodulatory effect of bergenin and norbergenin against adjuvant-induced arthritis - A flow cytometric study (2007) Journal of Ethnopharmacology, 112 (2), pp. 401-405NCCLS- National Commitee For Clinical Laboratory Standards. 1990. Approved standard M7-45. Standard methods for diluition antimicrobial susceptibility tests for bacteria that grow aerobically, Villanova, PaOgan, A.U., An isocoumarin from the bark of Sacoglottis gabonensis (1971) Phytochemistry, 10 (11), pp. 2832-2833Piacente, S., Constituents of Ardisia japonica and their in vitro anti-HIV activity (1996) Journal of Natural Products, 59 (6), pp. 565-569Prithiviraj, B., Singh, U.P., Manickam, M., Srivastava, J.S., Ray, A.B., Antifungal activity of bergenin, a constituent of (1997) Flueggea microcarpa. Plant Pathology, 46 (2), pp. 224-228Revilla, J., (2001) Plantas da Amazônia. Oportunidades Econômicas Sustentáveis, pp. 89-90. , Manaus, INPA/SEBRAESingh, U.P., Pandey, V.B., Singh, R.N., Singh, R.D.N., Antifungal activity of some new flavonenes and flavone glycosides of Echinops echinatus (1988) Canadian Journal of Botany, 66 (9), pp. 1354-1356Sumino, M., Sekine, T., Ruangrungsi, N., Igarashi, K., Ikegami, F., Ardisiphenols and other antioxidant principles from the fruits of Ardisia colorata (2002) Chemical & Pharmaceutical Bulletin, 50 (11), pp. 1484-1487Swarnalakshmi, T., Seth Raman, M.G., Sulochana, N., Arivudainambi, R., A note on the antiiflammatory activity of bergenin (1984) Current Science, 53 (17), pp. 917-917Takahachi, H., Kosaka, M., Watanabe, Y., Nakade, K., Fukuyama, Y., Synthesis and neuroprotective activity of bergenin derivatives with antioxidant activity (2003) Bioorganic & Medicinal Chemistry, 11 (8), pp. 1781-1788