Nitric Oxide Synthase-mediated Phytoalexin Accumulation In Soybean Cotyledons In Response To The Diaporthe Phaseolorum F. Sp. Meridionalis Elicitor

dc.creatorModolo L.V.
dc.creatorCunha F.Q.
dc.creatorBraga M.R.
dc.creatorSalgado I.
dc.date2002
dc.date2015-06-30T16:38:46Z
dc.date2015-11-26T15:30:28Z
dc.date2015-06-30T16:38:46Z
dc.date2015-11-26T15:30:28Z
dc.date.accessioned2018-03-28T22:38:57Z
dc.date.available2018-03-28T22:38:57Z
dc.identifier
dc.identifierPlant Physiology. , v. 130, n. 3, p. 1288 - 1297, 2002.
dc.identifier320889
dc.identifier10.1104/pp.005850
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0036851190&partnerID=40&md5=e3998b473350d22ef2b26ac0ae636bd1
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/101336
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/101336
dc.identifier2-s2.0-0036851190
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1261998
dc.descriptionPhytoalexin biosynthesis is part of the defense mechanism of soybean (Glycine max) plants against attack by the fungus Diaporthe phaseolorum f. sp. meridionalis (Dpm), the causal agent of stem canker disease. The treatment of soybean cotyledons with Dpm elicitor or with sodium nitroprusside (SNP), a nitric oxide (NO) donor, resulted in a high accumulation of phytoalexins. This response did not occur when SNP was replaced by ferricyanide, a structural analog of SNP devoid of the NO moiety. Phytoalexin accumulation induced by the fungal elicitor, but not by SNP, was prevented when cotyledons were pretreated with NO synthase (NOS) inhibitors. The Dpm elicitor also induced NOS activity in soybean tissues proximal to the site of inoculation. The induced NOS activity was Ca2+- and NADPH-dependent and was sensitive to the NOS inhibitors NG-nitro-L-arginine methyl ester, aminoguanidine, and L-N6-(iminoethyl) lysine. NOS activity was not observed in SNP-elicited tissues. An antibody to brain NOS labeled a 166-kD protein in elicited and nonelicited cotyledons. Isoflavones (daidzein and genistein), pterocarpans (glyceollins), and flavones (apigenin and luteolin) were identified after exposure to the elicitor or SNP, although the accumulation of glyceollins and apigenin was limited in SNP-elicited compared with fungal-elicited cotyledons. NOS activity preceded the accumulation of these flavonoids in tissues treated with the Dpm elicitor. The accumulation of these metabolites was faster in SNP-elicited than in fungal-elicited cotyledons. We conclude that the response of soybean cotyledons to Dpm elicitor involves NO formation via a constitutive NOS-like enzyme that triggers the biosynthesis of antimicrobial flavonoids.
dc.description130
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dc.description1288
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dc.languageen
dc.publisher
dc.relationPlant Physiology
dc.rightsfechado
dc.sourceScopus
dc.titleNitric Oxide Synthase-mediated Phytoalexin Accumulation In Soybean Cotyledons In Response To The Diaporthe Phaseolorum F. Sp. Meridionalis Elicitor
dc.typeArtículos de revistas


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