Artículos de revistas
Taurine Supplementation Increases Katp Channel Protein Content, Improving Ca2+ Handling And Insulin Secretion In Islets From Malnourished Mice Fed On A High-fat Diet
Registro en:
Amino Acids. Springer-verlag Wien, v. 46, n. 9, p. 2123 - 2136, 2014.
9394451
10.1007/s00726-014-1763-6
2-s2.0-84906323842
Autor
Vettorazzi J.F.
Ribeiro R.A.
Santos-Silva J.C.
Borck P.C.
Batista T.M.
Nardelli T.R.
Boschero A.C.
Carneiro E.M.
Institución
Resumen
Pancreatic β-cells are highly sensitive to suboptimal or excess nutrients, as occurs in protein-malnutrition and obesity. Taurine (Tau) improves insulin secretion in response to nutrients and depolarizing agents. Here, we assessed the expression and function of Cav and KATP channels in islets from malnourished mice fed on a high-fat diet (HFD) and supplemented with Tau. Weaned mice received a normal (C) or a low-protein diet (R) for 6 weeks. Half of each group were fed a HFD for 8 weeks without (CH, RH) or with 5 % Tau since weaning (CHT, RHT). Isolated islets from R mice showed lower insulin release with glucose and depolarizing stimuli. In CH islets, insulin secretion was increased and this was associated with enhanced KATP inhibition and Cav activity. RH islets secreted less insulin at high K+ concentration and showed enhanced KATP activity. Tau supplementation normalized K+-induced secretion and enhanced glucose-induced Ca 2+ influx in RHT islets. R islets presented lower Ca2+ influx in response to tolbutamide, and higher protein content and activity of the Kir6.2 subunit of the KATP. Tau increased the protein content of the α1.2 subunit of the Cav channels and the SNARE proteins SNAP-25 and Synt-1 in CHT islets, whereas in RHT, Kir6.2 and Synt-1 proteins were increased. In conclusion, impaired islet function in R islets is related to higher content and activity of the KATP channels. Tau treatment enhanced RHT islet secretory capacity by improving the protein expression and inhibition of the KATP channels and enhancing Synt-1 islet content. © 2014 Springer-Verlag. 46 9 2123 2136 Amaral, A.G., Rafacho, A., Machado De Oliveira, C.A., Batista, T.M., Ribeiro, R.A., Latorraca, M.Q., Boschero, A.C., Carneiro, E.M., Leucine supplementation augments insulin secretion in pancreatic islets of malnourished mice (2010) Pancreas, 39 (6), pp. 847-855 Andersson, S.A., Olsson, A.H., Esguerra, J.L., Heimann, E., Ladenvall, C., Edlund, A., Salehi, A., Eliasson, L., Reduced insulin secretion correlates with decreased expression of exocytotic genes in pancreatic islets from patients with type 2 diabetes (2012) Mol Cell Endocrinol, 364 (1-2), pp. 36-45 Araujo, E.P., De Souza, C.T., Ueno, M., Cintra, D.E., Bertolo, M.B., Carvalheira, J.B., Saad, M.J., Velloso, L.A., Infliximab restores glucose homeostasis in an animal model of diet-induced obesity and diabetes (2007) Endocrinology, 148 (12), pp. 5991-5997. , http://endo.endojournals.org/cgi/reprint/148/12/5991, DOI 10.1210/en.2007-0132 Barker, D.J.P., Hales, C.N., Fall, C.H.D., Osmond, C., Phipps, K., Clark, P.M.S., Type 2 (non-insulin-dependent) diabetes mellitus, hypertension and hyperlipidaemia (syndrome X): Relation to reduced fetal growth (1993) Diabetologia, 36 (1), pp. 62-67. , DOI 10.1007/BF00399095 Batista, T.M., Ribeiro, R.A., Amaral, A.G., De Oliveira, C.A., Boschero, A.C., Carneiro, E.M., Taurine supplementation restores glucose and carbachol-induced insulin secretion in islets from low-protein diet rats: Involvement of Ach-M3R, Synt 1 and SNAP-25 proteins (2012) J Nutr Biochem, 23 (3), pp. 306-312 Batista, T.M., Da Silva, P.M., Amaral, A.G., Ribeiro, R.A., Boschero, A.C., Carneiro, E.M., Taurine supplementation restores insulin secretion and reduces ER stress markers in protein-malnourished mice (2013) Adv Exp Med Biol, 776, pp. 129-139 Batista, T.M., Ribeiro, R.A., Da Silva, P.M., Camargo, R.L., Lollo, P.C., Boschero, A.C., Carneiro, E.M., Taurine supplementation improves liver glucose control in normal protein and malnourished mice fed a high-fat diet (2013) Mol Nutr Food Res, 57 (3), pp. 423-434. , doi:10.1002/mnfr.201200345 Bernardis, L.L., Patterson, B.D., Correlation between 'Lee index' and carcass fat content in weanling and adult female rats with hypothalamic lesions (1968) J Endocrinol, 40 (4), pp. 527-528 Best, L., Jarman, E., Brown, P.D., A dual action of saturated fatty acids on electrical activity in rat pancreatic beta-cells. Role of volume-regulated anion channel and KATP channel currents (2011) J Physiol, 589 (PART 6), pp. 1307-1316 Bol, V.V., Delattre, A.I., Reusens, B., Raes, M., Remacle, C., Forced catch-up growth after fetal protein restriction alters the adipose tissue gene expression program leading to obesity in adult mice (2009) Am J Physiol Regul Integr Comp Physiol, 297 (2), pp. R291-R299. , doi:10.1152/ajpregu.90497.2008 Boschero, A.C., Malaisse, W.J., Stimulus-secretion coupling of glucose-induced insulin release. XXIX. Regulation of 86Rb+ efflux from perifused islets (1979) American Journal of Physiology Endocrinology Metabolism and Gastrointestinal Physiology, 5 (2), pp. E139-E146 Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal Biochem, 72, pp. 248-254 Branstrom, R., Aspinwall, C.A., Valimaki, S., Ostensson, C.-G., Tibell, A., Eckhard, M., Brandhorst, H., Larsson, O., Long-Chain CoA esters activate human pancreatic beta-cell KATP channels: Potential role in Type 2 diabetes (2004) Diabetologia, 47 (2), pp. 277-283. , DOI 10.1007/s00125-003-1299-x Bustamante, J., Lobo, M.V., Alonso, F.J., Mukala, N.T., Gine, E., Solis, J.M., Tamarit-Rodriguez, J., Martin Del Rio, R., An osmotic-sensitive taurine pool is localized in rat pancreatic islet cells containing glucagon and somatostatin (2001) Am J Physiol Endocrinol Metab, 281 (6), pp. E1275-E1285 Camargo, R.L., Batista, T.M., Ribeiro, R.A., Velloso, L.A., Boschero, A.C., Carneiro, E.M., Effects of taurine supplementation upon food intake and central insulin signaling in malnourished mice fed on a high-fat diet (2013) Adv Exp Med Biol, 776, pp. 93-103. , doi:10.1007/978-1-4614-6093-0-10 Carneiro, E.M., Latorraca, M.Q., Araujo, E., Beltra, M., Oliveras, M.J., Navarro, M., Berna, G., Martin, F., Taurine supplementation modulates glucose homeostasis and islet function (2009) J Nutr Biochem, 20 (7), pp. 503-511 Cnop, M., Welsh, N., Jonas, J.-C., Jorns, A., Lenzen, S., Eizirik, D.L., Mechanisms of pancreatic beta-cell death in type 1 and type 2 diabetes: Many differences, few similarities (2005) Diabetes, 54 (SUPPL. 2), pp. S97-S107. , http://diabetes.diabetesjournals.org/cgi/reprint/54/suppl_2/S97, DOI 10.2337/diabetes.54.suppl-2.S97 Da Silva, P.M., Batista, T.M., Ribeiro, R.A., Zoppi, C.C., Boschero, A.C., Carneiro, E.M., Decreased insulin secretion in islets from protein malnourished rats is associated with impaired glutamate dehydrogenase function: Effect of leucine supplementation (2012) Metabolism, 61 (5), pp. 721-732 Delghingaro-Augusto, V., Ferreira, F., Bordin, S., Do Amaral, M.E., Toyama, M.H., Boschero, A.C., Carneiro, E.M., A low protein diet alters gene expression in rat pancreatic islets (2004) J Nutr, 134 (2), pp. 321-327 Ferreira, F., Filiputti, E., Arantes, V.C., Stoppiglia, L.F., Araujo, E.P., Delghingaro-Augusto, V., Latorraca, M.Q., Carneiro, E.M., Decreased cholinergic stimulation of insulin secretion by islets from rats fed a low protein diet is associated with reduced protein kinase Calpha expression (2003) Journal of Nutrition, 133 (3), pp. 695-699 Ferreira, F., Barbosa, H.C.L., Stoppiglia, L.F., Delghingaro-Augusto, V., Pereira, E.A., Boschero, A.C., Carneiro, E.M., Decreased Insulin Secretion in Islets from Rats Fed a Low Protein Diet Is Associated with a Reduced PKAalpha Expression (2004) Journal of Nutrition, 134 (1), pp. 63-67 Gribble, F.M., Reimann, F., Pharmacological modulation of K(ATP) channels (2002) Biochem Soc Trans, 30 (2), pp. 333-339 Hales, C.N., Barker, D.J., Type 2 (non-insulin-dependent) diabetes mellitus: The thrifty phenotype hypothesis (1992) Diabetologia, 35 (7), pp. 595-601 Han, J., Bae, J.H., Kim, S.-Y., Lee, H.-Y., Jang, B.-C., Lee, I.-K., Cho, C.-H., Song, D.-K., Taurine increases glucose sensitivity of UCP2-overexpressing beta-cells by ameliorating mitochondrial metabolism (2004) American Journal of Physiology - Endocrinology and Metabolism, 287 (5), pp. E1008-E1018. , DOI 10.1152/ajpendo.00008.2004 Hoppa, M.B., Collins, S., Ramracheya, R., Hodson, L., Amisten, S., Zhang, Q., Johnson, P., Rorsman, P., Chronic palmitate exposure inhibits insulin secretion by dissociation of Ca(2 +) channels from secretory granules (2009) Cell Metab, 10 (6), pp. 455-465 Iwashima, Y., Abiko, A., Ushikubi, F., Hata, A., Kaku, K., Sano, H., Eto, M., Downregulation of the voltage-dependent calcium channel (VDCC) beta-subunit mRNAs in pancreatic islets of type 2 diabetic rats (2001) Biochemical and Biophysical Research Communications, 280 (3), pp. 923-932. , DOI 10.1006/bbrc.2000.4122 Lee, S.H., Lee, H.Y., Kim, S.Y., Lee, I.K., Song, D.K., Enhancing effect of taurine on glucose response in UCP2-overexpressing beta cells (2004) Diabetes Res Clin Pract, 66 (SUPPL. 1), pp. S69-S74 Lim, J.-G., Lee, H.-Y., Yun, J.-E., Kim, S.-P., Park, J.-W., Suh, S.-I., Jang, B.-C., Song, D.-K., Taurine block of cloned ATP-sensitive K+ channels with different sulfonylurea receptor subunits expressed in Xenopus laevis oocytes (2004) Biochemical Pharmacology, 68 (5), pp. 901-910. , DOI 10.1016/j.bcp.2004.05.050, PII S0006295204003879 Moritz, W., Leech, C.A., Ferrer, J., Habener, J.F., Regulated expression of adenosine triphosphate-sensitive potassium channel subunits in pancreatic beta-cells (2001) Endocrinology, 142 (1), pp. 129-138. , DOI 10.1210/en.142.1.129 Nagamatsu, S., Nakamichi, Y., Yamamura, C., Matsushima, S., Watanabe, T., Ozawa, S., Furukawa, H., Ishida, H., Decreased expression of t-SNARE, syntaxin 1, and SNAP-25 in pancreatic beta-cells is involved in impaired insulin secretion from diabetic GK rat islets: Restoration of decreased t-SNARE proteins improves impaired insulin secretion (1999) Diabetes, 48 (12), pp. 2367-2373 Navarro, M., (2009) La Suplementación Con Taurina Modula la Homeostasis de la Glucosa Y la Función de Los Islotes Pancreáticos, , Universidad Pablo de Olavide Thesis Sevilla, España 2009 Olofsson, C.S., Salehi, A., Holm, C., Rorsman, P., Palmitate increases L-type Ca2+ currents and the size of the readily releasable granule pool in mouse pancreatic beta-cells (2004) Journal of Physiology, 557 (3), pp. 935-948. , DOI 10.1113/jphysiol.2004.066258 Park, E.-J., Bae, J.H., Kim, S.-Y., Lim, J.-G., Baek, W.-K., Kwon, T.K., Suh, S.-I., Song, D.-K., Inhibition of ATP-sensitive K+ channels by taurine through a benzamido-binding site on sulfonylurea receptor 1 (2004) Biochemical Pharmacology, 67 (6), pp. 1089-1096. , DOI 10.1016/j.bcp.2003.11.003, PII S0006295203008852 Peng, Z., Xiaolei, Z., Al-Sanaban, H., Chengrui, X., Shengyi, Y., Ghrelin inhibits insulin release by regulating the expression of inwardly rectifying potassium channel 6.2 in islets (2012) Am J Med Sci, 343 (3), pp. 215-219 Reis, M.A.B., Carneiro, E.M., Mello, M.A.R., Boschero, A.C., Saad, M.J.A., Velloso, L.A., Glucose-induced insulin secretion is impaired and insulin-induced phosphorylation of the insulin receptor and insulin receptor substrate-1 are increased in protein-deficient rats (1997) Journal of Nutrition, 127 (3), pp. 403-410 Remacle, C., Dumortier, O., Bol, V., Goosse, K., Romanus, P., Theys, N., Bouckenooghe, T., Reusens, B., Intrauterine programming of the endocrine pancreas (2007) Diabetes Obes Metab, 9 (SUPPL. 2), pp. 196-209. , doi:10.1111/j.1463-1326.2007.00790.x Ribeiro, R.A., Bonfleur, M.L., Amaral, A.G., Vanzela, E.C., Rocco, S.A., Boschero, A.C., Carneiro, E.M., Taurine supplementation enhances nutrient-induced insulin secretion in pancreatic mice islets (2009) Diabetes Metab Res Rev, 25 (4), pp. 370-379 Ribeiro, R.A., Vanzela, E.C., Oliveira, C.A., Bonfleur, M.L., Boschero, A.C., Carneiro, E.M., Taurine supplementation: Involvement of cholinergic/phospholipase C and protein kinase A pathways in potentiation of insulin secretion and Ca2 + handling in mouse pancreatic islets (2010) Br J Nutr, 104 (8), pp. 1148-1155 Ribeiro, R.A., Santos-Silva, J.C., Vettorazzi, J.F., Cotrim, B.B., Mobiolli, D.D., Boschero, A.C., Carneiro, E.M., Taurine supplementation prevents morpho-physiological alterations in high-fat diet mice pancreatic beta-cells (2012) Amino Acids, 43 (4), pp. 1791-1801 Rorsman, P., Braun, M., Zhang, Q., Regulation of calcium in pancreatic alpha- and beta-cells in health and disease (2012) Cell Calcium, 51 (3-4), pp. 300-308 Sandovici, I., Smith, N.H., Nitert, M.D., Ackers-Johnson, M., Uribe-Lewis, S., Ito, Y., Jones, R.H., Ozanne, S.E., Maternal diet and aging alter the epigenetic control of a promoter-enhancer interaction at the Hnf4a gene in rat pancreatic islets (2011) Proc Natl Acad Sci USA, 108 (13), pp. 5449-5454. , doi:10.1073/pnas.1019007108 Satoh, H., Sperelakis, N., Review of some actions of taurine on ion channels of cardiac muscle cells and others (1998) General Pharmacology, 30 (4), pp. 451-463. , DOI 10.1016/S0306-3623(97)00309-1, PII S0306362398003091 Smith, A.J., Partridge, C.J., Asipu, A., Mair, L.A., Hunter, M., Sivaprasadarao, A., Increased ATP-sensitive K+ channel expression during acute glucose deprivation (2006) Biochemical and Biophysical Research Communications, 348 (3), pp. 1123-1131. , DOI 10.1016/j.bbrc.2006.07.170, PII S0006291X06017487 Solon, C.S., Franci, D., Ignacio-Souza, L.M., Romanatto, T., Roman, E.A., Arruda, A.P., Morari, J., Velloso, L.A., Taurine enhances the anorexigenic effects of insulin in the hypothalamus of rats (2012) Amino Acids, 42 (6), pp. 2403-2410. , doi:10.1007/s00726-011-1045-5 Soriano, S., Gonzalez, A., Marroqui, L., Tuduri, E., Vieira, E., Amaral, A.G., Batista, T.M., Quesada, I., Reduced insulin secretion in protein malnourished mice is associated with multiple changes in the beta-cell stimulus-secretion coupling (2010) Endocrinology, 151 (8), pp. 3543-3554 Tokuyama, Y., Fan, Z., Furuta, H., Makielski, J.C., Polonsky, K.S., Bell, G.I., Yano, H., Rat inwardly rectifying potassium channel Kir6.2: Cloning, electrophysiological characterization, and decreased expression in pancreatic islets of male Zucker diabetic fatty rats (1996) Biochemical and Biophysical Research Communications, 220 (3), pp. 532-538. , DOI 10.1006/bbrc.1996.0439 Tricarico, D., Barbieri, M., Camerino, D.C., Taurine blocks ATP-sensitive potassium channels of rat skeletal muscle fibres interfering with the sulphonylurea receptor (2000) British Journal of Pharmacology, 130 (4), pp. 827-834 Winzell, M.S., Magnusson, C., Ahren, B., Temporal and dietary fat content-dependent islet adaptation to high-fat feeding-induced glucose intolerance in mice (2007) Metabolism: Clinical and Experimental, 56 (1), pp. 122-128. , DOI 10.1016/j.metabol.2006.09.008, PII S0026049506003398 Yang, S.N., Berggren, P.O., The role of voltage-gated calcium channels in pancreatic beta-cell physiology and pathophysiology (2006) Endocr Rev, 27 (6), pp. 621-676