There is evidence that soy consumption benefits health by contributing to the prevention of chronic diseases due several factors, including isoflavones present in this legume. The isoflavones from soybean are known to have several biological properties such as estrogenic, antimicrobial and antitumoral (breast and prostate) activities, besides inhibiting the activity of enzymes linked to the cellular division. These properties are shown to be more accentuated in the aglycone than glycoside forms. Their presence in soy products have been reported with a number of beneficial health effects. In addition,both USA and Brazil are great soybean producer in the World. The analysis of isoflavone form contents into the soy products take an important role in the understanding of consumer of these products. In this way, with the germination process, the soybean has a positive effect in improving nutritional status including isoflavones. However, the isoflavone content varies with the period of germination. The process of germination showed no significant difference in amount of glycitin, genistein, malonyl daidzin,malonyl genistin, daidzein, glycitein and genistein, although theirs numerical reductions after germination. There were only differences in the amount of daidzein and malonyl glycitin between the samples. Some authors have been reported that the total concentration of isoflavones increased after 1 day of germination and then slowly decrease these concentrations. This increase and decrease observed in the content of isoflavones may be associated with the conversion of isoflavones to other flavonoids. The germination time of 48 hours not improved the amount of isoflavones. Different cultivars present several amount of isoflavones and forms of isoflavones. When the grains were cooked to be soft the malonyl form decreased, and aglycone and glycoside forms increased. It was observed that the heating treatment transformed the malonylglucosides into glucoside isoflavones. After heat treatment at 121°C for 30 min, nearly all malonyl isoflavones were converted into glucoside, but acetylisoflavones were not detected via RP-HPLC analysis. On the other hand, gamma radiation also promoted some reduction on isoflavone profiles, but this effect depends of humidity into soy products. Irradiation from 60Co interfere at amount of isoflavones forms at doses up 2 kGy, glycosides and malonyl forms have tendency to down, but aglycones increase with radiation. For example, when soybean flour was studied, the gamma-radiation had a little effect (loss of 9.9%, w/w) in total isoflavone. However, soy isoflavones are likely to have the beneficial effects when found in aglycone forms in many processes will be proposed to obtain those aglycone isoflavones. © 2010 by Nova Science Publishers, Inc. All rights reserved. 225238Aguiar, C.L., Physical and biochemical transformation of soybean isoflavone (Glycine max L.) and the effect on biological activities (2004), PhD thesis. State University of Campinas, UNICAMP: Campinas, Brazil. (in Portuguese)Aguiar, C.L., Alencar, S.M., Pacheco, T.A.R.C., Park, Y.K., Perdas de isoflavonas durante processamento de alimentos de soja (2001), Proceedings of the 4th Simpósio Latino-Americano de Ciência de Alimentos, 99Aguiar, C.L., Baptista, A.S., Alencar, S.M., Haddad, R., Eberlin, M.N., Analysis of isoflavonoids from leguminous plant extracts by RPHPLC/DAD and eletrospray ionization mass spectrometry (2007) Intern. J. Food Sci. Nutr., 58, pp. 116-124Aguiar, C.L., Baptista, A.S., Walder, J.M.M., Tsai, S.M., Carrão-Panizzi, M.C., Kitajima, E.W., Changes in isoflavone profiles of soybean treated with gamma irradiation (2009) Intern J Food Sci Nutr, 60, pp. 387-394Ahmad, S., Patha, K.D.K., Nutritional changes in soybeans during germination (2000) J. Food Sci. Techn., 37, pp. 665-666Andlauer, W., Fürst, P., Antioxidative power of phytochemicals with special reference to cereals (1998) Cereal Foods World, 43, pp. 356-360Arditi, T., Meredith, T., Flowerman, P., Renewed interest in soy isoflavones and saponins (2000) Cereal Foods World, 45, pp. 414-417Baldasso, J.G., Araújo, M.M., Mancini-Filho, J., Detecção de soja irradiada pelo ensaio do Cometa e Germinação (2007), http://www.usp.br/siicusp/11osiicusp/ficha2054.htm, Conselho Nacional de Energia Nuclear/CNEN. IPEN. Centro de Tecnologia das Radiações, Acess:13 Jul 2007Barnes, S., Kirk, M., Coward, L., Isoflavones and their conjugates in soy foods: extraction conditions and analysis by HPLC-MS (1994) J Agric Food Chem, 42, pp. 2466-2474Berger, M., Rasolohery, C.A., Cazalis, R., Daydé, J., Isoflavone accumulation kinetics in soybeans seed cotyledons and hypocotyls: distinct pathways and genetic controls (2008) Crop Sci, 48, pp. 700-708Brown, J.P., Hydrolysis and esters (1998) Role of the gut flora in toxicity and cancer, pp. 109-144. , Eds. Rowland, IR, Academic PressCarrão-Panizzi, M.C., Beléia, A.D.P., Keisuke, K., Oliveira, M.C.N., Effects of genetics and environment on isoflavone content of soybean from different regions of Brazil (1999) Pesq Agropec Bras, 34, pp. 1787-1795Carrão-Panizzi, M.C., Keisuke, K., Beléia, A.D.P., Oliveira, M.C.N., Influence of growth locations on isoflavone contents in Brazilian soybean cultivars (1998) Breeding Sci, 48, pp. 409-413Charron, C.S., Allen, F.L., Johnson, R.D., Pantalone, V.R., Sams, C.E., Correlations of oil and protein with isoflavone concentration in soybean [Glycine max (L.) Merr.] (2005) J Agric Food Chem, 53, pp. 7128-7135Chiarello, M.D., Le Guerroué, J.L., Chagas, C.M.S., Franco, O.L., Bianchini, E., João, M.J., Influence of heat treatment and grain germination on the isoflavone profile of soy milk (2006) J Food Bioch, 30, pp. 234-247Coward, L., Barnes, N.C., Setchell, K.D.R., Barnes, S., Genistein, daidzein, and their beta-glycoside conjugates: antitumor isoflavones in soybean foods from American and Asian diets (1993) J Agric Food Chem, 41, pp. 1961-1967Crawford, L.M., Ruff, E.H., A review of the safety of cold pasteurization through irradiation (1996) Food Cont, 7, pp. 87-97Dhaubhadel, S., Farhangkhoee, M., Chapman, R., Identification and characterization of isoflavonoid specific glycosyltransferase and malonyltransferase from soybean seeds (2008) J Exper Bot, 59, pp. 981-994Dueñas, M., Hernández, T., Estrella, I., Fernández, D., Germination as a process to increase the polyphenol content and antioxidnt activity of lupin seeds (Lupines angustifolius L.) (2009) Food Chem, 117, pp. 599-607Fan, X., Argenta, L., Mattheis, J., Impacts of ionizing radiation on volatile production by ripening Gala apple fruit (2001) J Agric Food Chem, 49, pp. 254-262Friend, D.R., Chang, G.W., A colon-specific drug-delivery system based on drug glycosides and the glycosidases of colonic bacteria (1984) J Med Chem, 27, pp. 261-266Graham, T.L., Flavonoid and isoflavonoid distribution in developing soybean seedling tissues and in seed and root exudates (1991) Plant Phys, 95, pp. 594-603Grün, I.U., Adhikari, K., Li, C., Li, Y., Lin, B., Zhang, J., Fernando, L.N., Changes in the profile of genistein, daidzein, and their conjugates during thermal processing of tofu (2001) J Agric Food Chem, 49, pp. 2839-2843Hallman, G.J., Ionizing radiation quarantine treatments against tephritid fruit flies (1999) Postharvest Biol Technol, 16, pp. 93-106Hawksworth, G., Drasar, B.S., Hill, M.J., Intestinal bacteria and the hydrolysis of glycosidic bonds (1971) J Med Microbiol, 4, pp. 451-459Hsieh, H.C., Kao, T.H., Chen, B.H., A fast HPLC method for analysis of isoflavones in soybean (2005) J Liquid Chrom Related Techn, 27, pp. 315-324Wholesomeness of irradiated foods (1981), pp. 7-34. , FAO/IAEA/WHO Expert Committee, Report of World Health Organization Research Series No. 659. World Health Organization, GenevaKim, J.A., Hong, S.B., Jung, W.S., Yu, C.Y., Ma, K.H., Gwag, J.G., Chung, I.M., Comparison of isoflavones composition in seed, embryo, cotyledon and seed coat of cooked-with-rice and vegetable soybean (Glycine max L.) varieties (2007) Food Chem., 102, pp. 738-744Kuo, Y.H., Rozan, P., Lambein, F., Frias, J., Vidal-Valverde, C., Effects of different germination conditions on the contents of free protein and non-protein amino acids of commercial legumes (2004) Food Chem., 86, pp. 537-545Liggins, J., Bluck, L.J.C., Runswick, S., Atkinson, C., Coward, W.A., Bingham, S.A., Daidzein and genistein content of fruits and nuts (2000) J Nutr Biochem, 11, pp. 326-331Lin, P.Y., Lai, H.M., Bioactive compounds in legumes and their germinated products (2006) J Agric Food Chem, 54, pp. 3807-3814Lv, Q., Yang, Y., Zhao, Y., Gu, D., He, D., Yili, A., Ma, Q., Ito, Y., Comparative study on separation and purification of isoflavones from the seeds and sprouts of chickpea by high-seed countercurrent chromatography (2009) J Liquid Chrom Related Techn, 32, pp. 2879-2892Mahungu, S.M., Diaz-Mercado, S., Li, J., Schwenk, M., Singletary, K., Faller, J., Stability of isoflavones during extrusion processing of corn/soy mixture (1999) J Agric Food Chem, 47, pp. 279-284Martinez, A.P.C., Martinez, P.C.C., Souza, M.C., Canniatti-Brazaca, S.G., Alencar, S.M., Moreno, I.A., Odoni, T.L.C., Isoflavone contents in germinated soybeans (2008), http://www.usp.br/siicusp/Resumos/16Siicusp/4654.pdf, Simpósio Internacional de iniciação científica, 16. Piracicaba SP, Acess: jan. 12 2010Martinez, A.P.C., Martinez, P.C.C., Souza, M.C., Canniatti-Brazaca, S.G., Chemical change in soybean grains with germination Ciên Tecnol AlimMonje, M.C., Berger, M., Farines, V., Reybier, R., Verger, A., Daydé, J., Théodorout, V., Nepveu, F., Antioxidant capacity of cotyledons and germs of soybean in relation to their isoflavone content (2006) Food Techn Biotechn, 44, pp. 493-498Murphy, P.A., Barua, K., Hauck, C.C., Solvent extraction selection in the determination of isoflavones in soy foods (2002) J Chrom B: biomed Sci Appl, 77, pp. 129-138Noguchi, A., Saito, A., Homma, Y., Nakao, M., Sasaki, N., Nishino, T., Takahashi, S., Nakayama, T.A., UDP-glucose:isoflavone 7-Oglycosyltransferase from the roots of soybean (Glycine max) seedlings: Purification, gene cloning, phylogenetics, and na implication for an alternative strategy of enzyme catalysis (2007) J Biol Chem, 282, pp. 23581-23590Oufedjikh, H., Mahrouz, M., Lacroix, M., Amiot, M.J., Taccini, M., The influence of gamma irradiation on flavonoid content during storage of irradiated Clementina (1998) Radiat Phys Chem, 52, pp. 107-112Pandjaitan, N., Hettiarachchy, N., Ju, Z.Y., Enrichment of genistein in soy protein concentrate with beta-glucosidase (2000) J Food Sci., 65, pp. 403-407Park, Y.K., Aguiar, C.L., Alencar, S.M., Mascarenhas, H.A.A., Scamparini, A.R.P., Conversion of malonyl-beta-glycoside isoflavones into glycoside isoflavones in Brazilian soybeans (2002) Ciên Tecn Alim, 32, pp. 130-136Park, Y.K., Aguiar, C.L., Alencar, S.M., Mascarenhas, H.A.A., Scamparini, A.R.P., Survey of isoflavone contents in Brazilian soybean (2001) Cien Tecn Alim, 3, pp. 12-14Park, Y.K., Alencar, S.M., Nery, I.A., Aguiar, C.L., Sato, H.H., Enrichment of isoflavone aglycones in extracted soybean isoflavones by heat and fungal betaglucosidase (2001) Food Sci Industry, 34, pp. 14-19Park, Y.K., Aguiar, C.L., Alencar, S.M., Nery, I.A., Sato, H.H., Extraction of isoflavones from defatted soy flour by ethanol and conversion of glucoside isoflavones to aglycones by fungal beta-glucosidase (2001), p. 164. , IFT Annual MeetingPaucar-Menacho, L.M., Berhow, M.A., Mandarino, J.M.G., Mejia, E.G.D., Chang, Y.K., Optimisation of germination time and temperature on the concentration of bioactive compounds in Brazilian soybean cultivar BRS 133 using response surface methodology (2010) Food Chem, 119, pp. 636-642Phommalth, S., Jeong, Y.S., Kim, Y.H., Dhakal, K.H., Hwang, Y.H., Effects of light treatment on isoplavone content of germinated soybean seeds (2008) J Agric Food Chem, 56, pp. 10123-10128Ribeiro, M.L.L., Mandarino, J.M.G., Carrão-Panizzi, M.C., Oliveira, M.C.N., Campo, C.B.H., Nepomuceno, A.L., Ida, E.I., β-glycosidase activity and isoflavone content in germinated soybeans radicles and cotyledons (2006) J Food Bioch, 30, pp. 453-465Sangronis, E., Machado, C.J., Influence of germination on the nutritional quality of Phaseolus vulgaris and Cajanus cajan (2007) LWT-Food Sci Tech, 40, pp. 116-120Simonne, A.H., Smith, M., Weaver, D.B., Vail, T., Barnes, S., Wei, C.I., Retention and changes of soy isoflavones and carotenoids in immature soybean seeds (Edamame) during processing (2000) J Agric Food Chem, 48, pp. 6061-6069Sokhey, A.S., Hanna, M.A., Properties of irradiated starches (1993) Food Structure, 12, pp. 397-410Suzuki, H., Takahashi, S., Watanabe, R., Fukushima, Y., Fujita, N., Noguchi, A., Yokoyama, R., Nakayama, T., An isoflavone conjugatehydrolyzing beta-glucosidase from the roots of soybean (Glycine max) seedlings-Purification, gene cloning, phylogenetics, and cellular localization (2006) J Biol Chem, 281, pp. 30251-30259Thayer, D.W., Rajkowski, K.T., New development in irradiation of fresh fruits and vegetables (1999) Food Technol, 53, pp. 62-65Tozette, B.N., Toledo, T.C.F., Alencar, S.M., Moreno, I.A.M., Odoni, T.L.C., Effects of ionizing radiation from 60Co at isoflavone concentration in cooked soybeans (2008), http://www.usp.br/siicusp/Resumos/16Siicusp/2585.pdf, Simpósio Internacional de iniciação científica, 16. Piracicaba SP, Acess: jan., 12, 2010Troszynska, A., Szymkiewicz, A., Wolejszo, A., The effects of germination on the sensory quality and immunoreactive properties of pea (Pisum sativum L.) and soybean (Glycine max) (2007) J Food Qual, 30, pp. 1083-1100Tsangalis, D., Ashton, J.F., Stojanovska, L., Wilcox, G., Shah, N.P., Development of an isoflavone aglycone-enriched soymilk using soy germ, soy protein isolate and bifidobasteria (2004) Food Res Intern, 37, pp. 301-312Urbano, G., Aranda, P., Vílchez, A., Aranda, C., Cabrera, L., Porres, J.M., López-Jurado, M., Effects of germination on the composition and nutritive value of proteins in Pisum sativum, L (2005) Food Chem, 93, pp. 671-679Vitti, D.M.S.S., Del Mastro, N.L., Kikuchi, O.K., Nogueira, N.L., Electron irradiation of high fiber by-products: effects on chemical composition and digestibility (1998) Sci. Agric., 55, pp. 159-171Wang, H.J., Murphy, P.A., Isoflavone content in commercial soybean foods (1994) J Agric Food Chem, 42, pp. 1666-1673Wu, E.S., Loch, J.T., Toder, B.H., Borrelli, A.R., Gawlak, D., Radov, L.A., Gensmantel, N.P., Flavones. 3. Synthesis, biological activities, and conformational analysis of isoflavone derivatives and related compounds (1992) J Med Chem, 35, pp. 3519-3525Xu, X., Harris, K.S., Wang, H.J., Murphy, P.A., Hendrich, S., Bioavailability of soybean isoflavonas depends upon gut microflora in women (1995) J Nutr., 125, pp. 2307-2315Yuan, J.P., Liu, Y.B., Peng, J., Wang, J.H., Liu, X., Changes of isoflavone profile in the hypocotyls and colyledons of soybeans during dry heating and germination (2009) J Agric Food Chem, 57, pp. 9002-9010Zhu, D., Hettiarachchy, N.S., Horax, R., Chen, P., Isoflavone contents in germinated soybean seeds (2005) Plant Foods Human Nutr, 60, pp. 147-151