Optical Tweezers Force Measurements To Study Parasites Chemotaxis

dc.creatorDe Thomaz A.A.
dc.creatorPozzo L.Y.
dc.creatorFontes A.
dc.creatorAlmeida D.B.
dc.creatorStahl C.V.
dc.creatorSantos-Mallet J.R.
dc.creatorGomes S.A.O.
dc.creatorFeder D.
dc.creatorAyres D.C.
dc.creatorGiorgio S.
dc.creatorCesar C.L.
dc.date2009
dc.date2015-06-26T13:32:32Z
dc.date2015-11-26T15:29:02Z
dc.date2015-06-26T13:32:32Z
dc.date2015-11-26T15:29:02Z
dc.date.accessioned2018-03-28T22:37:45Z
dc.date.available2018-03-28T22:37:45Z
dc.identifier9780819476432
dc.identifierProgress In Biomedical Optics And Imaging - Proceedings Of Spie. , v. 7367, n. , p. - , 2009.
dc.identifier16057422
dc.identifier10.1117/12.831480
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-70349988091&partnerID=40&md5=5b6ecf826a4792673fd6211d7ff88955
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/91575
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/91575
dc.identifier2-s2.0-70349988091
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1261742
dc.descriptionIn this work, we propose a methodology to study microorganisms chemotaxis in real time using an Optical Tweezers system. Optical Tweezers allowed real time measurements of the force vectors, strength and direction, of living parasites under chemical or other kinds of gradients. This seems to be the ideal tool to perform observations of taxis response of cells and microorganisms with high sensitivity to capture instantaneous responses to a given stimulus. Forces involved in the movement of unicellular parasites are very small, in the femto-pico-Newton range, about the same order of magnitude of the forces generated in an Optical Tweezers. We applied this methodology to investigate the Leishmania amazonensis (L. amazonensis) and Trypanossoma cruzi (T. cruzi) under distinct situations. ©2009 Optical Society of America.
dc.description7367
dc.description
dc.description
dc.description
dc.descriptionhttp://www.who.int/tdr/diseases/chagas/diseaseinfo.htmlWorld Health Organization. 2002. Annex 3: Burden of disease in DALYs by cause, sex and mortality stratum in WHO regions, estimates for 2001. The World Health report. Geneva: WHO 2002: 192-197Fontes, A., Giorgio, S., de Castro Jr., A.B., Neto, V.M., Pozzo, L.Y., Marques, G.P., Barbosa, L.C., Cesar, C.L., Determination of femtonewton forces and fluid viscosity using optical tweezers: Application to Leishmania amazonensis (2005) Proceedings of SPIE, 5699, pp. 419-425
dc.descriptionAlves, C.R., Albuquerque-Cunha, J.M., Mello, C.B., Garcia d, E.S., Nogueira, N.F., Bourguingnon, S.C., de Souza, W., Gonzalez, M.S., Trypanosoma cruzi: Attachment to perimicrovillar membrane glycoproteins of Rhodnius prolixus (2007) Experimental Parasitology, 116, pp. 44-52
dc.descriptionCortez, M.G.R., Gonzalez, M.S., Cabral, M.M.O., Garcia, E.S., Azambuja, P., Dynamic development of Trypanosoma cruzi in Rhodnius prolixus: Role of decapitation and ecdysone therapy (2002) ParasitologyResearch, 88, pp. 697-703
dc.descriptionKohidai, L., Chemotaxis: The proper physiological response to evaluate phylogeny of signal molecules (1999) Acta Biol Hung, 50, pp. 375-394
dc.descriptionBagorda, A., Parent, C.A., Eukaryotic chemotaxis at a glance (2008) J. Cell Science, 121, pp. 2621-2624
dc.descriptionwww.biophysics.org/education/williams.pdf
dc.languageen
dc.publisher
dc.relationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
dc.rightsfechado
dc.sourceScopus
dc.titleOptical Tweezers Force Measurements To Study Parasites Chemotaxis
dc.typeActas de congresos


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