Graphene And Carbon Nanotube Nanocomposite For Gene Transfection

dc.creatorHollanda L.M.
dc.creatorLobo A.O.
dc.creatorLancellotti M.
dc.creatorBerni E.
dc.creatorCorat E.J.
dc.creatorZanin H.
dc.date2014
dc.date2015-06-25T17:50:03Z
dc.date2015-11-26T15:28:25Z
dc.date2015-06-25T17:50:03Z
dc.date2015-11-26T15:28:25Z
dc.date.accessioned2018-03-28T22:37:08Z
dc.date.available2018-03-28T22:37:08Z
dc.identifier
dc.identifierMaterials Science And Engineering C. , v. 39, n. 1, p. 288 - 298, 2014.
dc.identifier9284931
dc.identifier10.1016/j.msec.2014.03.002
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84897879767&partnerID=40&md5=34318353fed9707e1156b70b7167d25c
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/85757
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/85757
dc.identifier2-s2.0-84897879767
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1261597
dc.descriptionGraphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also known to attach oxygen content groups on the MWCNT surfaces, changing their hydrophobicity. The nanocomposite was characterised by high resolution scanning electron microscopy; energy-dispersive X-ray, Fourier transform infrared and Raman spectroscopies, as well as zeta potential and particle size analyses using dynamic light scattering. BET adsorption isotherms showed the GCN to have an effective surface area of 38.5 m2/g. The GCN and pIRES plasmid conjugated with the GFP gene, forming π-stacking when dispersed in water by magnetic stirring, resulting in a helical wrap. The measured zeta potential confirmed that the plasmid was connected to the nanocomposite. The NIH-3T3 and NG97 cell lines could phagocytize this wrap. The gene transfection was characterised by fluorescent protein produced in the cells and pictured by fluorescent microscopy. Before application, we studied GCN cell viability in NIH-3T3 and NG97 line cells using both MTT and Neutral Red uptake assays. Our results suggest that GCN has moderate stability behaviour as colloid solution and has great potential as a gene carrier agent in non-viral based therapy, with low cytotoxicity and good transfection efficiency. © 2014 Elsevier B.V.
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dc.languageen
dc.publisher
dc.relationMaterials Science and Engineering C
dc.rightsfechado
dc.sourceScopus
dc.titleGraphene And Carbon Nanotube Nanocomposite For Gene Transfection
dc.typeArtículos de revistas


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