dc.creatorda Silva Fernandes M.
dc.creatorKabuki D.Y.
dc.creatorKuaye A.Y.
dc.date2015
dc.date2015-06-25T12:50:53Z
dc.date2015-11-26T15:27:43Z
dc.date2015-06-25T12:50:53Z
dc.date2015-11-26T15:27:43Z
dc.date.accessioned2018-03-28T22:36:23Z
dc.date.available2018-03-28T22:36:23Z
dc.identifier
dc.identifierInternational Journal Of Food Microbiology. Elsevier, v. 200, n. , p. 5 - 12, 2015.
dc.identifier1681605
dc.identifier10.1016/j.ijfoodmicro.2015.01.003
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84921990593&partnerID=40&md5=4cc5121094571ac7397f6934572a9d5b
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/85185
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/85185
dc.identifier2-s2.0-84921990593
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1261413
dc.descriptionThe formation of mono-species biofilm (Listeria monocytogenes) and multi-species biofilms (Enterococcus faecium, Enterococcus faecalis, and L. monocytogenes) was evaluated. In addition, the effectiveness of sanitation procedures for the control of the multi-species biofilm also was evaluated. The biofilms were grown on stainless steel coupons at various incubation temperatures (7, 25 and 39°C) and contact times (0, 1, 2, 4, 6 and 8days). In all tests, at 7°C, the microbial counts were below 0.4 log CFU/cm2 and not characteristic of biofilms. In mono-species biofilm, the counts of L. monocytogenes after 8days of contact were 4.1 and 2.8 log CFU/cm2 at 25 and 39°C, respectively. In the multi-species biofilms, Enterococcus spp. were present at counts of 8 log CFU/cm2 at 25 and 39°C after 8days of contact. However, the L. monocytogenes in multi-species biofilms was significantly affected by the presence of Enterococcus spp. and by temperature. At 25°C, the growth of L. monocytogenes biofilms was favored in multi-species cultures, with counts above 6 log CFU/cm2 after 8days of contact. In contrast, at 39°C, a negative effect was observed for L. monocytogenes biofilm growth in mixed cultures, with a significant reduction in counts over time and values below 0.4 log CFU/cm2 starting at day 4. Anionic tensioactive cleaning complemented with another procedure (acid cleaning, disinfection or acid cleaning+disinfection) eliminated the multi-species biofilms under all conditions tested (counts of all micro-organisms<0.4 log CFU/cm2). Peracetic acid was the most effective disinfectant, eliminating the multi-species biofilms under all tested conditions (counts of the all microorganisms <0.4 log CFU/cm2). In contrast, biguanide was the least effective disinfectant, failing to eliminate biofilms under all the test conditions. monocytogenes favored biofilm formation in multi-species cultures at 25°C. monocytogenes biofilm in mixed cultures at 39°C.
dc.description200
dc.description
dc.description5
dc.description12
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dc.languageen
dc.publisherElsevier
dc.relationInternational Journal of Food Microbiology
dc.rightsfechado
dc.sourceScopus
dc.titleBehavior Of Listeria Monocytogenes In A Multi-species Biofilm With Enterococcus Faecalis And Enterococcus Faecium And Control Through Sanitation Procedures
dc.typeArtículos de revistas


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