Proteome Of The Phytopathogen Xanthomonas Citri Subsp. Citri: A Global Expression Profile

dc.creatorSoares M.R.
dc.creatorFacincani A.P.
dc.creatorFerreira R.M.
dc.creatorMoreira L.M.
dc.creatorde Oliveira J.C.F.
dc.creatorFerro J.A.
dc.creatorFerro M.I.T.
dc.creatorMeneghini R.
dc.creatorGozzo F.C.
dc.date2010
dc.date2015-06-26T12:37:40Z
dc.date2015-11-26T15:27:34Z
dc.date2015-06-26T12:37:40Z
dc.date2015-11-26T15:27:34Z
dc.date.accessioned2018-03-28T22:36:16Z
dc.date.available2018-03-28T22:36:16Z
dc.identifier
dc.identifierProteome Science. , v. 8, n. , p. - , 2010.
dc.identifier14775956
dc.identifier10.1186/1477-5956-8-55
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-78049520842&partnerID=40&md5=0ace355ab9e3c4d464b91cde1dd16c86
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/91226
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/91226
dc.identifier2-s2.0-78049520842
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1261385
dc.descriptionBackground: Citrus canker is a disease caused by Xantomonas citri subsp.citri (Xac), and has emerged as one of the major threats to the worldwide citrus crop because it affects all commercial citrus varieties, decreases the production and quality of the fruits and can spread rapidly in citrus growing areas. In this work, the first proteome of Xac was analyzed using two methodologies, two-dimensional liquid chromatography (2D LC) and tandem mass spectrometry (MS/MS).Results: In order to gain insight into the metabolism of Xac, cells were grown on two different media (NB - Nutrient Broth and TSE - Tryptone Sucrose broth enriched with glutamic acid), and proteins were proteolyzed with trypsin and examined by 2D LC-MS/MS. Approximately 39% of all predicted proteins by annotation of Xac were identified with their component peptides unambiguously assigned to tandem mass spectra. The proteins, about 1,100, were distributed in all annotated functional categories.Conclusions: This is the first proteomic reference map for the most aggressive strain of Xanthomonas pathogen of all orange varieties. The compilation of metabolic pathways involved with bacterial growth showed that Xac expresses a complete central and intermediary metabolism, replication, transcription and translation machineries and regulation factors, distinct membrane transporters (ABC, MFS and pumps) and receptors (MCP, TonB dependent and metabolites acquisition), two-component systems (sensor and regulatory components) and response regulators. These data corroborate the growth curve in vitro and are the first reports indicating that many of these genome annotated genes are translated into operative in Xac. This proteomic analysis also provided information regarding the influence of culture medium on growth and protein expression of Xac. © 2010 Soares et al; licensee BioMed Central Ltd.
dc.description8
dc.description
dc.description
dc.description
dc.descriptionDelcher, A.L., Harmon, D., Kasif, S., White, O., Salzberg, S.L., Improved microbial gene identification with GLIMMER (1999) Nucleic Acids Res, 27, pp. 4636-4641. , 10.1093/nar/27.23.4636, 148753, 10556321
dc.descriptionGalperin, M.Y., Koonin, E.V., Conserved hypothetical' proteins: prioritization of targets for experimental study (2004) Nucleic Acids Res, 32, pp. 5452-5463. , 10.1093/nar/gkh885, 524295, 15479782
dc.descriptionPeng, J., Gygi, S.P., Proteomics: the Move to Mixtures (2001) J Mass Spectrom, 36, pp. 1083-1091. , 10.1002/jms.229, 11747101
dc.descriptionWatt, S.A., Wilke, A., Patschkowski, T., Niehaus, K., Comprehensive analysis of the extracellular proteins from Xanthomonas campestris pv. campestris B100 (2005) Proteomics, 5, pp. 153-167. , 10.1002/pmic.200400905, 15619296
dc.descriptionChung, W.J., Shu, H.Y., Lu, C.Y., Wu, C.Y., Tseng, Y.H., Tsai, S.F., Lin, C.H., Qualitative and comparative proteomic analysis of Xanthomonas campestris pv. campestris 17 (2007) Proteomics, 7, pp. 2047-2058. , 10.1002/pmic.200600647, 17566974
dc.descriptionAndrade, A.E., Silva, L.P., Pereira, J.L., Noronha, E.F., Reis, F.B., Bloch, C., Santos, M.F., Mehta, A., In vivo proteome analysis of Xanthomonas campestris pv. campestris in the interaction with the host plant Brassica oleracea (2008) FEMS Microbiol Lett, 281, pp. 167-174. , 10.1111/j.1574-6968.2008.01090.x, 18318710
dc.descriptionSidhu, V.K., Vorholter, F.J., Niehaus, K., Watt, S.A., Analysis of outer membrane vesicle associated proteins isolated from the plant pathogenic bacterium Xanthomonas campestris pv. campestris (2008) BMC Microbiol, 8, p. 87. , 10.1186/1471-2180-8-87, 2438364, 18518965
dc.descriptionda Silva, A.C., Ferro, J.A., Reinach, F.C., Farah, C.S., Furlan, L.R., Quaggio, R.B., Monteiro-Vitorello, C.B., Alves, L.M.C., Comparison of the genomes of two Xanthomonas pathogens with differing host specificities (2002) Nature, 417, pp. 459-463. , 10.1038/417459a, 12024217
dc.descriptionGoodman, H.M., Ecker, J.R., Dean, C., The genome of Arabidopsis thaliana (1995) Proc Natl Acad Sci USA, 92, pp. 10831-10835. , 10.1073/pnas.92.24.10831, 40525, 7479893
dc.descriptionSchaad, N.W., Postnikova, E., Lacy, G.H., Sechler, A., Agarkova, I., Stromberg, P.E., Stromberg, V.K., Vidaver, A.K., Reclassification of Xanthomonas campestris pv. citri (ex Hasse 1915) Dye 1978 forms A, B/C/D, and E as X. smithii subsp. citri (ex Hasse) sp. nov. nom. rev. comb. nov., X. fuscans subsp. aurantifolii (ex Gabriel 1989) sp. nov. nom. rev. comb. nov., and X. alfalfae subsp. citrumelo (ex Riker and Jones) Gabriel et al., 1989 sp. nov. nom. rev. comb. nov.
dc.descriptionX. campestris pv malvacearum (ex smith 1901) (2005) Syst Appl Microbiol, 28, pp. 494-518. , 10.1016/j.syapm.2005.03.017, 16104350
dc.descriptionSchaad, N.W., Postnikova, E., Lacy, G., Sechler, A., Agarkova, I., Stromberg, P.E., Stromberg, V.K., Vidaver, A.K., Emended classification of xanthomonad pathogens on citrus (2006) Syst Appl Microbiol, 29, pp. 690-695. , 10.1016/j.syapm.2006.08.001, 17183629
dc.descriptionStall, R.E., Civerolo, E.L., Xanthomonas campestris pv. citri: cause of citrus canker (1993), pp. 48-50. , Chapman and Hall, London, Swings JG, Civerolo ELGottwald, T.R., Graham, J.H., Compendium of Citrus Diseases (2000), pp. 5-7. , Am Phytopathol Soc, São Paulo, Timmer LW, Garnsey SM, Graham JHMoreira, L.M., de Souza, R.F., Almeida, N.F., Setubal, J.C., Oliveira, J.C., Furlan, L.R., Ferro, J.A., da Silva, A.C., Comparative genomics analyses of citrus-associated bacteria (2004) Annu Rev Phytophathol, 42, pp. 163-184
dc.descriptionMoreira, L.M., De Souza, R.F., Digiampietri, L.A., Da Silva, A.C.R., Setubal, J.C., Comparative analyses of Xanthomonas and Xylella complete genomes (2005) Omics, 9, pp. 43-76. , 10.1089/omi.2005.9.43, 15805778
dc.descriptionMoreira, L.M., Almeida, N.F., Potnis, N., Digiampietri, L.A., Adi, S.S., Bortolossi, L.C., da Silva, A.C., Oliveira, J.C., Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp. aurantifolii (2010) BMC Genomics, 11, p. 238. , 10.1186/1471-2164-11-238, 20388224
dc.descriptionMehta, A., Rosato, Y.B., A simple method for in vivo expression studies of Xanthomonas axonopodis pv. citri (2003) Curr Microbiol, 47, pp. 400-403
dc.descriptionAlegria, M.C., Souza, D.P., Andrade, M.O., Docena, C., Khater, L., Ramos, C.H., da Silva, A.C., Farah, C.S., Identification of new protein-protein interactions involving the products of the chromosome- and plasmid-encoded type IV secretion loci of the phytopathogen Xanthomonas axonopodis pv. citri (2005) J Bacteriol, 187, pp. 2315-2325. , 10.1128/JB.187.7.2315-2325.2005, 15774874
dc.descriptionZhu, W., MaGbanua, M.M., White, F.F., Identification of two novel hrp-associated genes in the hrp gene cluster of Xanthomonas oryzae pv. oryzae (2000) J Bacteriol, 182, pp. 1844-1853. , 10.1128/JB.182.7.1844-1853.2000, 101866, 10714988
dc.descriptionSchulte, R., Bonas, U., A Xanthomonas Pathogenicity Locus Is Induced by Sucrose and Sulfur-Containing Amino Acids (1992) Plant Cell, 4, pp. 79-86. , 10.1105/tpc.4.1.79, 160108, 12297631
dc.descriptionLee, S.W., Han, S.W., Bartley, L.E., Ronald, P.C., Unique characteristics of Xanthomonas oryzae pv. oryzae AvrXa21 and implications for plant innate immunity (2006) Proc Natl Acad Sci USA, 103, pp. 18395-18400. , 10.1073/pnas.0605508103, 1693675, 17082309
dc.descriptionWashburn, M.P., Wolters, D., Yates, J.R., Large-scale analysis of the yeast proteome by multidimensional protein identification technology (2001) Nat Biotechnol, 19, pp. 242-247. , 10.1038/85686, 11231557
dc.descriptionLink, A.J., Eng, J., Schieltz, D.M., Carmack, E., Mize, G.J., Morris, D.R., Garvik, B.M., Yates, J.R., Direct analysis of protein complexes using mass spectrometry (1999) Nat Biotechnol, 17, pp. 676-682. , 10.1038/10890, 10404161
dc.descriptionWu, C.C., MacCoss, M.J., Howell, K.E., Yates, J.R., A method for the comprehensive proteomic analysis of membrane proteins (2003) Nat Biotechnol, 21, pp. 532-538. , 10.1038/nbt819, 12692561
dc.descriptionTaoka, M., Yamauchi, Y., Shinkawa, T., Kaji, H., Motohashi, W., Nakayama, H., Takahashi, N., Isobe, T., Only a small subset of the horizontally transferred chromosomal genes in Escherichia coli are translated into proteins (2004) Mol Cell Proteomics, 3, pp. 780-787. , 10.1074/mcp.M400030-MCP200, 15115803
dc.descriptionBonas, U., Hrp genes of phytopathogenic bacteria (1994) Curr Top Microbiol Imunol, 192, pp. 79-98
dc.descriptionAltschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J., Basic local alignment search tool (1990) J Mol Biol, 215, pp. 403-410
dc.descriptionLaia, M.L., Moreira, L.M., Dezajacomo, J., Brigati, J.B., Ferreira, C.B., Ferro, M.I., Silva, A.C., Oliveira, J.C., New genes of Xanthomonas citri subsp. citri involved in pathogenesis and adaptation revealed by a transposon-based mutant library (2009) BMC Microbiol, 9, p. 12. , 10.1186/1471-2180-9-12, 2653504, 19149882
dc.descriptionCapy, P., Gasperi, G., Christian, B., Claude, B., Stress and transposable elements: co-evolution or useful parasites? (2000) Heredity, 85, pp. 101-106. , 10.1046/j.1365-2540.2000.00751.x, 11012710
dc.descriptionGrandbastien, M.A., Activation of plant retrotransposons under stress conditions (1998) Trends in Plant Science, 3, pp. 181-187
dc.descriptionAlegria, M.C., Docena, C., Khater, L., Ramos, C., da Silva, A.C., Farah, C.S., New protein-protein interactions identified for the regulatory and structural components and substrates of the type III Secretion system of the phytopathogen Xanthomonas axonopodis pathovar citri (2004) J Bacteriol, 186, pp. 6186-6197. , 10.1128/JB.186.18.6186-6197.2004, 515140, 15342589
dc.descriptionBlanvillain, S., Meyer, D., Boulanger, A., Lautier, M., Guynet, C., Denancé, N., Vasse, J., Arlat, M., Plant carbohydrate scavenging through tonb-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria (2007) PLoS One, 2, pp. e224. , 10.1371/journal.pone.0000224, 1790865, 17311090
dc.descriptionGottig, N., Garavaglia, B.S., Garofalo, C.G., Orellano, E.G., Ottado, J., A filamentous hemagglutinin-like protein of Xanthomonas axonopodis pv. citri, the phytopathogen responsible for citrus canker, is involved in bacterial virulence (2009) PLoS One, 4, pp. e4358. , 10.1371/journal.pone.0004358, 2632755, 19194503
dc.descriptionChen, L.L., Identification of genomic islands in six plant pathogens (2006) Gene, 374, pp. 134-141. , 10.1016/j.gene.2006.01.029, 16581205
dc.descriptionTobes, R., Pareja, E., Bacterial repetitive extragenic palindromic sequences are DNA targets for Insertion Sequence elements (2006) BMC Genomics, 7, p. 62. , 10.1186/1471-2164-7-62, 1525189, 16563168
dc.descriptionRossier, O., Van den Ackerveken, G., Bonas, U., HrpB2 and HrpF from Xanthomonas are type III-secreted proteins and essential for pathogenicity and recognition by the host plant (2000) Mol Microbiol, 38, pp. 828-838. , 10.1046/j.1365-2958.2000.02173.x, 11115117
dc.descriptionAlegria, M.C., Docena, C., Khater, L., Ramos, C.H., da Silva, A.C., Farah, C.S., New protein-protein interactions identified for the regulatory and structural components and substrates of the type III secretion system of the phytopathogen Xanthomonas axonopodis pv. citri (2004) J of Bacteriol, 186, pp. 6186-6197
dc.descriptionAlegria, M.C., Souza, D.P., Andrade, M.O., Docena, C., Khater, L., Ramos, C.H., da Silva, A.C., Farah, C.S., Identification of new protein-protein interactions involving the products of the chromosome- and plasmid-encoded type IV secretion loci of the phytopathogen Xanthomonas axonopodis pv. citri (2005) J Bacteriol, 187, pp. 2315-2325. , 10.1128/JB.187.7.2315-2325.2005, 1065226, 15774874
dc.descriptionAlfano, J.R., Collmer, A., TYPE III SECRETION SYSTEM EFFECTOR PROTEINS: Double Agents in Bacterial Disease and Plant Defense (2004) Annu Rev Phytopathol, 42, pp. 385-414. , 10.1146/annurev.phyto.42.040103.110731, 15283671
dc.descriptionLu, G.T., Ma, Z.F., Hu, J.R., Tang, D.J., He, Y.Q., Feng, J.X., Tang, J.L., A novel locus involved in extracellular polysaccharide production and virulence of Xanthomonas campestris pathovar campestris (2007) Microbiology, 157, pp. 737-746
dc.descriptionHenke, J.M., Bassler, B.L., Quorum sensing regulates type III secretion in Vibrio harveyi and Vibrio parahaemolyticus (2004) J Bacteriol, 186, pp. 3794-3805. , 10.1128/JB.186.12.3794-3805.2004, 419960, 15175293
dc.descriptionSperandio, V., Mellies, J.L., Nguyen, W., Shin, S., Kaper, J.B., Quorum sensing controls expression of the type III secretion gene transcription and protein secretion in enterohemorrhagic and enteropathogenic Escherichia coli (1999) Proc Natl Acad Sci USA, 96, pp. 15196-15201. , 10.1073/pnas.96.26.15196, 24796, 10611361
dc.descriptionLiu, H., Coulthurst, S.J., Pritchard, L., Hedley, P.E., Ravensdale, M., Humphris, S., Burr, T., Birch, P.R., Quorum sensing coordinates brute force and stealth modes of infection in the plant pathogen Pectobacterium atrosepticum (2008) PLoS Pathog, 4, pp. e1000093. , 10.1371/journal.ppat.1000093, 2413422, 18566662
dc.descriptionYap, M.N., Yang, C.H., Barak, J.D., Jahn, C.E., Charkowski, A.O., The Erwinia chrysanthemi type III secretion system is required for multicellular behavior (2005) J Bacteriol, 187, pp. 639-648. , 10.1128/JB.187.2.639-648.2005, 543537, 15629935
dc.descriptionMoreira, L.M., Almeida, N.F., Potnis, N., Digiampietri, L.A., Adi, S.S., Bortolossi, L.C., da Silva, A.C., Oliveira, J.C., Novel insights into the genomic basis of citrus canker based on the genome sequences of two strains of Xanthomonas fuscans subsp. aurantifolii (2010) BMC Genomics, 11, p. 238. , 10.1186/1471-2164-11-238, 2883993, 20388224
dc.languageen
dc.publisher
dc.relationProteome Science
dc.rightsaberto
dc.sourceScopus
dc.titleProteome Of The Phytopathogen Xanthomonas Citri Subsp. Citri: A Global Expression Profile
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución