| dc.creator | Fernandes J.C.B. | |
| dc.creator | Kubota L.T. | |
| dc.creator | Neto G.D.O. | |
| dc.date | 1999 | |
| dc.date | 2015-06-30T15:18:52Z | |
| dc.date | 2015-11-26T15:23:36Z | |
| dc.date | 2015-06-30T15:18:52Z | |
| dc.date | 2015-11-26T15:23:36Z | |
| dc.date.accessioned | 2018-03-28T22:32:30Z | |
| dc.date.available | 2018-03-28T22:32:30Z | |
| dc.identifier | | |
| dc.identifier | Analytica Chimica Acta. , v. 385, n. 1-3, p. 3 - 12, 1999. | |
| dc.identifier | 32670 | |
| dc.identifier | 10.1016/S0003-2670(98)00327-4 | |
| dc.identifier | http://www.scopus.com/inward/record.url?eid=2-s2.0-0032999632&partnerID=40&md5=e4c4876660cf6a6e95a2f2ce6d4a52c9 | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/100851 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/100851 | |
| dc.identifier | 2-s2.0-0032999632 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1260503 | |
| dc.description | The development of a potentiometric biosensor for L-ascorbic acid based on ascorbate oxidase is described. The enzyme was extracted of the epicarp of Cucumis sativus L. fruit and immobilized in a graphite/epoxy electrode by occlusion in a poly(ethylene-co-vinyl acetate) matrix (EVA 40%m/m). The electrode showed a sub-Nerntian response (slope of 50.3±0.6mV), between 8.0x10-6 and 4.5x10-4moll-1 for ascorbate, when 0.1moll-1 KH2PO4 buffer at pH 5.0 and 25.0°C was employed. The biosensor was continuously tested, during 15 days (about 300 analyses), and no decrease in the response was observed, for vitamin C determination in pharmaceuticals samples, without any previous treatment. The biosensor was stable for at least 1 month when stored in a refrigerator. The relative standard deviation was about 4% compared to the spectrophotometric method. The response time was about 5min at room temperature. Copyright (C) 1999 Elsevier Science B.V. | |
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| dc.language | en | |
| dc.publisher | | |
| dc.relation | Analytica Chimica Acta | |
| dc.rights | fechado | |
| dc.source | Scopus | |
| dc.title | Potentiometric Biosensor For L-ascorbic Acid Based On Ascorbate Oxidase Of Natural Source Immobilized On Ethylene-vinylacetate Membrane | |
| dc.type | Actas de congresos | |
