| dc.creator | Floriano R.S. | |
| dc.creator | Rocha T. | |
| dc.creator | Carregari V.C. | |
| dc.creator | Marangoni S. | |
| dc.creator | Da Cruz-Hofling M.A. | |
| dc.creator | Hyslop S. | |
| dc.creator | Rodrigues-Simioni L. | |
| dc.creator | Rowan E.G. | |
| dc.date | 2015 | |
| dc.date | 2015-06-25T12:55:49Z | |
| dc.date | 2015-11-26T15:21:08Z | |
| dc.date | 2015-06-25T12:55:49Z | |
| dc.date | 2015-11-26T15:21:08Z | |
| dc.date.accessioned | 2018-03-28T22:30:39Z | |
| dc.date.available | 2018-03-28T22:30:39Z | |
| dc.identifier | | |
| dc.identifier | Toxicon. Elsevier Ltd, v. 96, n. , p. 24 - 37, 2015. | |
| dc.identifier | 410101 | |
| dc.identifier | 10.1016/j.toxicon.2015.01.001 | |
| dc.identifier | http://www.scopus.com/inward/record.url?eid=2-s2.0-84921454644&partnerID=40&md5=03098c49cdd6549629e225e562451736 | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/85652 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/85652 | |
| dc.identifier | 2-s2.0-84921454644 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1260153 | |
| dc.description | The presynaptic action of Bothriopsis bilineata smaragdina (forest viper) venom and Bbil-TX, an Asp49 PLA2 from this venom, was examined in detail in mouse phrenic nerve-muscle (PND) preparations in vitro and in a neuroblastoma cell line (SK-N-SH) in order to gain a better insight into the mechanism of action of the venom and associated Asp49 PLA2. In low Ca2+ solution, venom (3 μg/ml) caused a quadriphasic response in PND twitch height whilst at 10 μg/ml the venom additionally induced an abrupt and marked initial contracture followed by neuromuscular facilitation, rhythmic oscillations of nerve-evoked twitches, alterations in baseline and progressive blockade. The venom slowed the relaxation phase of muscle twitches. In low Ca2+, Bbil-TX [210 nM (3 μg/ml)] caused a progressive increase in PND twitch amplitude but no change in the decay time constant. Venom (10 μg/ml) and Bbil-TX (210 nM) caused minor changes in the compound action potential (CAP) amplitude recorded from sciatic nerve preparations, with no significant effect on rise time and latency; tetrodotoxin (3.1 nM) blocked the CAP at the end of the experiments. In mouse triangularis sterni nerve-muscle (TSn-m) preparations, venom (10 μg/ml) and Bbil-TX (210 nM) significantly reduced the perineural waveform associated with the outward K+ current while the amplitude of the inward Na+ current was not significantly affected. Bbil-TX (210 nM) caused a progressive increase in the quantal content of TSn-m preparations maintained in low Ca2+ solution. Venom (3 μg/ml) and toxin (210 nM) increased the calcium fluorescence in SK-N-SH neuroblastoma cells loaded with Fluo3 AM and maintained in low or normal Ca2+ solution. In normal Ca2+, the increase in fluorescence amplitude was accompanied by irregular and frequent calcium transients. In TSn-m preparations loaded with Fluo4 AM, venom (10 μg/ml) caused an immediate increase in intracellular Ca2+ followed by oscillations in fluorescence and muscle contracture; Bbil-TX did not change the calcium fluorescence in TSn-m preparations. Immunohistochemical analysis of toxin-treated PND preparations revealed labeling of junctional ACh receptors but a loss of the presynaptic proteins synaptophysin and SNAP25. Together, these data confirm the presynaptic action of Bbil-TX and show that it involves modulation of K+ channel activity and presynaptic protein expression. | |
| dc.description | 96 | |
| dc.description | | |
| dc.description | 24 | |
| dc.description | 37 | |
| dc.description | Borja-Oliveira, C.R., Kassab, B.H., Soares, A.M., Toyama, M.H., Giglio, J.R., Marangoni, S., Re, L., Rodrigues-Simioni, L., Purification and N-terminal sequencing of two presynaptic neurotoxic PLA2, neuwieditoxin-I and neuwieditoxin-II, from Bothrops neuwiedi pauloensis (jararaca pintada) venom (2007) J. Venom. Anim. Toxins Incl. Trop. Dis., 13, pp. 103-121 | |
| dc.description | Braga, M.F., Anderson, A.J., Harvey, A.L., Rowan, E.G., Apparent block of K+ currents in mouse motor nerve terminals by tetrodotoxin, mu-conotoxin and reduced external sodium (1992) Br. J. Pharmacol., 106, pp. 91-94 | |
| dc.description | Calgarotto, A.K., Damico, D.C.S., Ponce-Soto, L.A., Baldasso, P.A., Da Silva, S.L., Souza, G.H.M.F., Eberlin, M.N., Marangoni, S., Biological and biochemical characterization of a new basic phospholipase A2 BmTX-I isolated from Bothrops moojeni snake venom (2008) Toxicon, 51, pp. 1509-1519 | |
| dc.description | Campbell, J.A., Lamar, W.W., (2004) Venomous Reptiles of the Western Hemisphere, 2. , Comstock Publishing Associates/Cornell University Press Ithaca | |
| dc.description | Carregari, V.C., Floriano, R.S., Rodrigues-Simioni, L., Winck, F.V., Baldasso, P.A., Ponce-Soto, L.A., Marangoni, S., Biochemical, pharmacological, and structural characterization of new basic PLA2 Bbil-TX from Bothriopsis bilineata snake venom (2013) BioMed. Res. Int., 2013. , Article ID 612649, 12 pages | |
| dc.description | Chang, C.C., Su, M.J., Presynaptic toxicity of the histidine-modified, phospholipase A2-inactive β-bungarotoxin, crotoxin and notexin (1982) Toxicon, 20, pp. 895-905 | |
| dc.description | Chang, C.C., Lee, J.D., Eaker, D., Fohlman, J., The presynaptic neuromuscular blocking action of taipoxin. A comparison with β-bungarotoxin and crotoxin (1977) Toxicon, 15, pp. 571-576 | |
| dc.description | Cogo, J.C., Prado-Franceschi, J., Cruz-Höfling, M.A., Corrado, A.P., Rodrigues-Simioni, L., Effect of Bothrops insularis venom on the mouse and chick nerve-muscle preparation (1993) Toxicon, 31, pp. 1237-1247 | |
| dc.description | Cogo, J.C., Lilla, S., Souza, G.H.M.F., Hyslop, S., Nucci, G., Purification, sequencing and structural analysis of two acidic phospholipases A2 from the venom of Bothrops insularis (jararaca ilhoa) (2006) Biochimie, 88, pp. 1947-1959 | |
| dc.description | Correia-De-Sá, P., Noronha-Matos, J.B., Timóteo, M.A., Ferreirinha, F., Marques, P., Soares, A.M., Carvalho, C., Gallacci, M., Bothropstoxin-I reduces evoked acetylcholine release from rat motor nerve terminals: Radiochemical and real-time video-microscopy studies (2013) Toxicon, 61, pp. 16-25 | |
| dc.description | Dal Belo, C.A., Leite, G.B., Toyama, M.H., Marangoni, S., Corrado, A.P., Fontana, M.D., Southand, A., Rodrigues-Simioni, L., Pharmacological and structural characterization of a novel phospholipase A2 from Micrurus dumerilii carinicauda venom (2005) Toxicon, 46, pp. 736-750 | |
| dc.description | Dempster, J., Computer analysis of electrophysiological signals (1988) Microcomputers in Physiology: A Practical Approach, pp. 51-93. , P.J. Frazer, IRL Press Oxford | |
| dc.description | Dixon, R.W., Harris, J.B., Nerve terminal damage by β-bungarotoxin - Its clinical significance (1999) Am. J. Pathol., 154, pp. 447-455 | |
| dc.description | Dreyer, F., Penner, R., The actions of presynaptic snake toxins on membrane currents of mouse motor nerve terminals (1987) J. Physiol., 386, pp. 455-463 | |
| dc.description | Dumitru, D., Amato, A.A., (2008) Neuromuscular Disorders, pp. 1127-1212. , McGraw-Hill Education Columbus, OH | |
| dc.description | Faiz, A., Ghose, A., Ahsan, F., Rahman, R., Amin, R., Hassan, M.U., Chowdhury, A.W., Warrell, D.A., The greater black krait (Bungarus niger), a newly recognized cause of neuro-myotoxic snake bite envenoming in Bangladesh (2010) Brain, 133, pp. 3181-3193 | |
| dc.description | Fatehi, M., Harvey, A.L., Rowan, E.G., Characterisation of the effects of depolarising toxins on nerve terminal action potentials: Apparent block of presynaptic potassium currents (1998) Toxicon, 36, pp. 115-129 | |
| dc.description | Fathi, B., Rowan, E.G., Harvey, A.L., The facilitatory actions of snake venom phospholipase A2 neurotoxins at the neuromuscular junction are not mediated through voltage-gated K+ channels (2001) Toxicon, 39, pp. 1871-1882 | |
| dc.description | Fathi, B., Harvey, A.L., Rowan, E.G., The effect of temperature on the effects of the phospholipase A2 neurotoxins β-bungarotoxin and taipoxin at the neuromuscular junction (2013) Toxicon, 70, pp. 86-89 | |
| dc.description | Floriano, R.S., Carregari, V.C., Abreu, V.A., Kenzo-Kagawa, B., Ponce-Soto, L.A., Cruz-Höfling, M.A., Hyslop, S., Rodrigues-Simioni, L., Pharmacological study of a new Asp49 phospholipase A2 (Bbil-TX) isolated from Bothriopsis bilineata smargadina (forest viper) venom in vertebrate neuromuscular preparations (2013) Toxicon, 69, pp. 191-199 | |
| dc.description | Fohlman, J., Eaker, D., Karlsson, E., Thesleff, S., Taipoxin, an extremely potent presynaptic neurotoxin from the venom of the Australian snake taipan (Oxyuranus S. Scutellatus) (1976) Eur. J. Biochem., 68, pp. 457-469 | |
| dc.description | França, F.O.S., Málaque, C.M.S., Acidente botrópico (2003) Animais Peçonhentos No Brasil: Biologia, Clínica e Terapêutica Dos Acidentes, pp. 72-86. , J.L.C. Cardoso, F.O.S. França, F.H. Wen, C.M.S. Málaque, V. Haddad Jr. Sarvier/FAPESP São Paulo | |
| dc.description | Galbiatti, C., Rocha, T., Randazzo-Moura, P., Ponce-Soto, L.A., Marangoni, S., Cruz-Höfling, M.A., Rodrigues-Simioni, L., Pharmacological and partial biochemical characterization of a new non-myotoxic neurotoxic PLA2 (Bmaj-9) isolated from Bothrops marajoensis snake venom (2012) J. Venom. Anim. Toxins Incl. Trop. Dis., 18, pp. 62-72 | |
| dc.description | Gallacci, M., Cavalcante, W.L., Understanding the in vitro neuromuscular activity of snake venom Lys49 phospholipase A2 homologues (2010) Toxicon, 55, pp. 1-11 | |
| dc.description | Harris, J.B., Neuron to nerve terminals: Aspects of neuropathology and toxinology (2006) Braz. J. Morphol. Sci., 23, pp. 195-216 | |
| dc.description | Harvey, A.L., Marshall, R.J., Karlsson, E., Effects of purified cardiotoxins from the Thailand cobra (Naja naja siamensis) on isolated skeletal and cardiac muscle preparations (1982) Toxicon, 20, pp. 379-396 | |
| dc.description | Lin-Shiau, S.Y., Fu, W.M., Liu, S.H., Ni, C.T., Studies on contractures induced in mouse diaphragm by caffeine and cupric and selenite ions (1989) Arch. Int. Pharmacodyn. Ther., 300, pp. 265-280 | |
| dc.description | Logantha, S.J.R.J., Cruickshank, S.F., Rowan, E.G., Drummond, R.M., Spontaneous and electrically evoked Ca2+ transients in cardiomyocytes of the rat pulmonary vein (2010) Cell Calcium, 48, pp. 150-160 | |
| dc.description | Lomonte, B., Angulo, Y., Calderón, L., An overview of lysine-49 phospholipase A2 myotoxins from crotalid snake venoms and their structural determinants of myotoxic action (2003) Toxicon, 42, pp. 885-901 | |
| dc.description | McArdle, J.J., Angaut-Petit, D., Mallart, A., Bournaud, R., Faille, L., Brigant, J.L., Advantages of the triangularis sterni muscle of the mouse for investigations of synaptic phenomena (1981) J. Neurosci. Methods, 4, pp. 109-115 | |
| dc.description | Montecucco, C., Rossetto, O., How do presynaptic PLA2 neurotoxins block nerve terminals? (2000) Trends Biochem. Sci., 25, pp. 266-270 | |
| dc.description | Moraes, D.S., De Abreu, V.A., Rostelato-Ferreira, S., Leite, G.B., Cruz-Höfling, M.A., Travaglia-Cardoso, S.R., Hyslop, S., Rodrigues-Simioni, L., Neuromuscular activity of Bothrops alcatraz snake venom in chick biventer cervicis preparations (2012) Toxicon, 59, pp. 294-299 | |
| dc.description | Oshima-Franco, Y., Leite, G.B., Belo, C.A., Hyslop, S., Prado-Franceschi, J., Cintra, A.C., Giglio, J.R., Rodrigues-Simioni, L., The presynaptic activity of bothropstoxin-I, a myotoxin from Bothrops jararacussu snake venom (2004) Basic Clin. Pharmacol. Toxicol., 95, pp. 175-182 | |
| dc.description | Penner, R., Dreyer, F., Two different presynaptic calcium currents in mouse nerve terminals (1986) Pflugers Arch., 406, pp. 190-197 | |
| dc.description | Ponce-Soto, L.A., Barros, J.C., Marangoni, S., Hernandez, S., Dal Belo, C.A., Corrado, A.P., Hyslop, S., Rodrigues-Simioni, L., Neuromuscular activity of BaTX, a presynaptic basic PLA2 isolated from Bothrops alternatus snake venom (2009) Comp. Biochem. Physiol. C, 150, pp. 291-297 | |
| dc.description | Pungerčar, J., Križaj, I., Understanding the molecular mechanism underlying the presynaptic toxicity of secreted phospholipases A2 (2007) Toxicon, 50, pp. 871-892 | |
| dc.description | Rocha, T., De Souza, B.M., Palma, M.S., Cruz-Höfling, M.A., Harris, J.B., The neurotoxicological effects of mastoparan Polybia-MPII at the murine neuromuscular junction: An ultrastructural and immunocytochemical study (2009) Histochem. Cell Biol., 132, pp. 395-404 | |
| dc.description | Rodrigues-Simioni, L., Zamunér, S.R., Cogo, J.C., Borja-Oliveira, C.R., Prado-Franceschi, J., Cruz-Höfling, M.A., Corrado, A.P., Pharmacological evidence for a presynaptic action of venoms from Bothrops insularis (jararaca ilhoa) and Bothrops neuwiedi (jararaca pintada) (2004) Toxicon, 43, pp. 633-638 | |
| dc.description | Rodrigues-Simioni, L., Floriano, R.S., Rostelato-Ferreira, S., Sousa, N.C., Marangoni, S., Ponce-Soto, L.A., Carregari, V.C., Hyslop, S., Presynaptic action of Bothriopsis bilineata smargadina (forest viper) venom in vitro (2011) Toxicon, 58, pp. 140-145 | |
| dc.description | Rossetto, O., Montecucco, C., Presynaptic neurotoxins with enzymatic activities (2008) Handb. Exp. Pharmacol., 184, pp. 129-170 | |
| dc.description | Rossetto, O., Rigoni, M., Montecucco, C., Different mechanism of blockade of neuroexocytosis by presynaptic neurotoxins (2004) Toxicol. Lett., 149, pp. 91-101 | |
| dc.description | Rowan, E.G., What does β-bungarotoxin do at the neuromuscular junction? (2001) Toxicon, 39, pp. 107-118 | |
| dc.description | Rowan, E.G., Harvey, A.L., Potassium channel blocking actions of β-bungarotoxin and related toxins on mouse and frog motor nerve terminals (1988) Br. J. Pharmacol., 94, pp. 839-847 | |
| dc.description | Rowan, E.G., Pemberton, K.E., Harvey, A.L., On the blockade of acetylcholine release at mouse motor nerve terminals by β-bungarotoxin and crotoxin (1990) Br. J. Pharmacol., 100, pp. 301-304 | |
| dc.description | Simpson, L.L., Lautenslager, G.T., Kaiser, I.I., Middlebrook, J.L., Identification of the site at which phospholipase A2 neurotoxins localise to produce their neuromuscular blocking effects (1993) Toxicon, 31, pp. 13-26 | |
| dc.description | Soares, A.M., Giglio, J.R., Chemical modifications on phospholipases A2 from snake venoms: Effects on catalytic and pharmacological properties (2003) Toxicon, 42, pp. 855-868 | |
| dc.description | Su, M.J., Chang, C.C., Presynaptic effects of snake venom toxins which have phospholipase A2 activity (β-bungarotoxin, taipoxin, crotoxin) (1984) Toxicon, 22, pp. 631-640 | |
| dc.description | Tedesco, E., Rigonia, M., Caccin, P., Grishin, E., Rossetto, O., Montecucco, C., Calcium overload in nerve terminals of cultured neurons intoxicated by α-latrotoxin and snake PLA2 neurotoxins (2009) Toxicon, 54, pp. 138-144 | |
| dc.description | Warrell, D.A., Snakebites in Central and South America: Epidemiology, clinical features and clinical management (2004) Venomous Reptiles of the Western Hemisphere, 2, pp. 709-761. , J.A. Campbell, W.W. Lamar, Comstock Publishing Associates/Cornell University Press Ithaca | |
| dc.description | Zamunér, S.R., Cruz-Höfling, M.A., Corrado, A.P., Hyslop, S., Rodrigues-Simioni, L., Comparison of the neurotoxic and myotoxic effects of Brazilian Bothrops venoms and their neutralization by commercial antivenom (2004) Toxicon, 44, pp. 259-271 | |
| dc.language | en | |
| dc.publisher | Elsevier Ltd | |
| dc.relation | Toxicon | |
| dc.rights | fechado | |
| dc.source | Scopus | |
| dc.title | The Neuromuscular Activity Of Bothriopsis Bilineata Smaragdina (forest Viper) Venom And Its Toxin Bbil-tx (asp49 Phospholipase A2) On Isolated Mouse Nerve-muscle Preparations | |
| dc.type | Artículos de revistas | |
