Brasil | Actas de congresos

Combined Nonlinear Laser Imaging (two-photon Excitation Fluorescence, Second And Third-harmonic Generation, And Fluorescence Lifetime Imaging Microscopies) In Ovarian Tumors

dc.creatorAdur J.
dc.creatorPelegati V.B.
dc.creatorDe Thomaz A.A.
dc.creatorBottcher-Luiz F.
dc.creatorAndrade L.A.L.A.
dc.creatorAlmeida D.B.
dc.creatorCarvalho H.F.
dc.creatorCesar C.L.
dc.date2012
dc.date2015-06-25T20:23:49Z
dc.date2015-11-26T15:18:58Z
dc.date2015-06-25T20:23:49Z
dc.date2015-11-26T15:18:58Z
dc.date.accessioned2018-03-28T22:28:32Z
dc.date.available2018-03-28T22:28:32Z
dc.identifier9780819488695
dc.identifierProgress In Biomedical Optics And Imaging - Proceedings Of Spie. , v. 8226, n. , p. - , 2012.
dc.identifier16057422
dc.identifier10.1117/12.906945
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84859621917&partnerID=40&md5=4adcdcc6af31dd716de4e5937f6d354f
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/90095
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/90095
dc.identifier2-s2.0-84859621917
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1259648
dc.descriptionWe applied Two-photon Excited Fluorescence (TPEF), Second/Third Harmonic Generation (SHG and THG) and Fluorescence Lifetime Imaging (FLIM) Non Linear Optics (NLO) Laser-Scanning Microscopy within the same imaging platform to evaluate their use as a diagnostic tool in ovarian tumors. We assess of applicability of this multimodal approach to perform a pathological evaluation of serous and mucinous tumors in human samples. The combination of TPEF-SHG-THG imaging provided complementary information about the interface epithelium/stromal, such as the transformation of epithelium surface (THG) and the overall fibrillar tissue architecture (SHG). The fact that H&E staining is the standard method used in clinical pathology and that the stored samples are usually fixed makes it important a re-evaluation of these samples with NLO microscopy to compare new results with a library of already existing samples. FLIM, however, depends on the chemical environment around the fluorophors that was completely changed after fixation; therefore it only makes sense in unstained samples. Our FLIM results in unstained samples demonstrate that it is possible to discriminate healthy epithelia from serous or mucinous epithelia. Qualitative and quantitative analysis of the different imaging modalities used showed that multimodal nonlinear microscopy has the potential to differentiate between cancerous and healthy ovarian tissue. © 2012 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).
dc.description8226
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dc.descriptionThe Society of Photo-Optical Instrumentation Engineers (SPIE),Becker and Hickel GmbH,Boston Electronics Corp.,Carl Zeiss MicroImaging GmbH,Chroma Technologies Corp.
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dc.languageen
dc.publisher
dc.relationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
dc.rightsfechado
dc.sourceScopus
dc.titleCombined Nonlinear Laser Imaging (two-photon Excitation Fluorescence, Second And Third-harmonic Generation, And Fluorescence Lifetime Imaging Microscopies) In Ovarian Tumors
dc.typeActas de congresos


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