Small-angle X-ray Scattering And Structural Modeling Of Full-length: Cellobiohydrolase I From Trichoderma Harzianum

dc.creatorLima L.H.F.
dc.creatorSerpa V.I.
dc.creatorRosseto F.R.
dc.creatorSartori G.R.
dc.creatorde Oliveira Neto M.
dc.creatorMartinez L.
dc.creatorPolikarpov I.
dc.date2013
dc.date2015-06-25T19:17:59Z
dc.date2015-11-26T15:15:48Z
dc.date2015-06-25T19:17:59Z
dc.date2015-11-26T15:15:48Z
dc.date.accessioned2018-03-28T22:25:36Z
dc.date.available2018-03-28T22:25:36Z
dc.identifier
dc.identifierCellulose. , v. 20, n. 4, p. 1573 - 1585, 2013.
dc.identifier9690239
dc.identifier10.1007/s10570-013-9933-3
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84881023746&partnerID=40&md5=6eb4bbef88e26c681ae1d6dd0d462f36
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/89651
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/89651
dc.identifier2-s2.0-84881023746
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1259094
dc.descriptionCellobiohydrolases hydrolyze cellulose releasing cellobiose units. They are very important for a number of biotechnological applications, such as, for example, production of cellulosic ethanol and cotton fiber processing. The Trichoderma cellobiohydrolase I (CBH1 or Cel7A) is an industrially important exocellulase. It exhibits a typical two domain architecture, with a small C-terminal cellulose-binding domain and a large N-terminal catalytic core domain, connected by an O-glycosylated linker peptide. The mechanism by which the linker mediates the concerted action of the two domains remains a conundrum. Here, we probe the protein shape and domain organization of the CBH1 of Trichoderma harzianum (ThCel7A) by small angle X-ray scattering (SAXS) and structural modeling. Our SAXS data shows that ThCel7A linker is partially-extended in solution. Structural modeling suggests that this linker conformation is stabilized by inter- and intra-molecular interactions involving the linker peptide and its O-glycosylations. © 2013 Springer Science+Business Media Dordrecht.
dc.description20
dc.description4
dc.description1573
dc.description1585
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dc.languageen
dc.publisher
dc.relationCellulose
dc.rightsfechado
dc.sourceScopus
dc.titleSmall-angle X-ray Scattering And Structural Modeling Of Full-length: Cellobiohydrolase I From Trichoderma Harzianum
dc.typeArtículos de revistas


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