We describe a female patient of 1. year and 5. months-old, referred for genetic evaluation due to neuropsychomotor delay, hearing impairment and dysmorphic features. The patient presents a partial chromosome 21 monosomy (q11.2→q21.3) in combination with a chromosome 3p terminal monosomy (p25.3→pter) due to an unbalanced de novo translocation. The translocation was confirmed by fluorescence in situ hybridization (FISH) and the breakpoints were mapped with high resolution array. After the combined analyses with these techniques the final karyotype was defined as 45,XX,der(3)t(3;21)(p25.3;q21.3)dn,-21.ish der(3)t(3;21)(RP11-329A2-,RP11-439F4-,RP11-95E11-,CTB-63H24. +).arr 3p26.3p25.3(35,333-10,888,738)). ×. 1,21q11.2q21.3(13,354,643-27,357,765). ×. 1. Analysis of microsatellite DNA markers pointed to a paternal origin for the chromosome rearrangement. 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