Artículos de revistas
Identification Of Nuclear Factor-κb Sites In The Slc2a4 Gene Promoter
Registro en:
Molecular And Cellular Endocrinology. , v. 370, n. 1/Fev, p. 87 - 95, 2013.
3037207
10.1016/j.mce.2013.01.019
2-s2.0-84876402852
Autor
Furuya D.T.
Neri E.A.
Poletto A.C.
Anhe G.F.
Freitas H.S.
Campello R.S.
Reboucas N.A.
Machado U.F.
Institución
Resumen
Glucose transporter GLUT4 protein, codified by Slc2a4 gene plays a key role in glycemic homeostasis. Insulin resistance, as in obesity, has been associated to inflammatory state, in which decreased GLUT4 is a feature. Inflammatory NF-κB transcriptional factor has been proposed as a repressor of Slc2a4; although, the binding site(s) in Slc2a4 promoter and the direct repressor effect have never been reported yet. A motif-based sequence analysis of mouse Slc2a4 promoter revealed two putative κB sites located inside -83/-62 and -134/-113. bp. Eletrophoretic mobility assay showed that p50 and p65 NF-κB subunits bind to both putative κB sites. Chromatin immunoprecipitation assay using genomic DNA from adipocytes confirmed p50- and p65-binding to Slc2a4 promoter. Moreover, transfection experiments revealed that NF-κB binds to the -134/-113. bp region of the mouse Slc2a4 gene promoter, inhibiting the Slc2a4 gene transcription. 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