The aim of this work was to evaluate the bulk oil and biodiesel production by cyanobacteria Phormidium sp. using cassava wastewater as culture medium. The study focused on optimization of the cultivation parameters (temperature and C/N ratio), on the evaluation of different operational modes of bioreactor (batch, fed-batch and continuous) and on the analysis of the biofuel quality. The results indicate that temperatures of 30°C and C/N ratio of 68 improved the performance of system. Additionally, the continuous cultivation optimizes the rate of formation of product, reaching biomass productivities of 320.1 mg/L h in parallel to oil productivities of 43.8 mg/L h. Finally, the fuel properties of the biodiesel indicated an ester content of 99.98%, a cetane number of 57.28, an iodine value of 45.22 gl2 100 g-1, a degree of unsaturation of 49.27%, and a cold filter plugging point of 61.43 °C. The technological route developed indicates potential to sustainable production of bulk oil and biodiesel, through the minimization of water and chemicals demand needed to support such bioprocess.31482487Wohlgemuth, R., The locks and keys to industrial biotechnology (2009) New Biotechnol., 25 (4), pp. 204-213. , http://dx.doi.org/10.1016/j.nbt.2009.01.002.19429540Clark, J., Deswarte, F., Farmer, T., The integration of green chemistry into future biorefineries (2009) Biofuels Bioprod. Biorefin., 3 (1), pp. 72-90. , http://dx.doi.org/10.1002/bbb.119Charpentier, J.C., Process intensification by miniaturization (2005) Chem. Eng. Technol., 28 (3), pp. 255-258. , http://dx.doi.org/10.1002/ceat.200407026Jacob-Lopes, E., Franco, T.T., From oil refinery to microalgal biorefinery (2013) J. CO2 Util., 2, pp. 1-7. , http://dx.doi.org/10.1016/j.jcou.2013.06.001Queiroz, M.I., Hornes, M.O., Da Silva-Manetti, A.G., Jacob-Lopes, E., Single-cell oil production by cyanobacterium Aphanothece microscopica Nägeli cultivated heterotrophically in fish processing wastewater (2011) Appl. Energy, 88 (10), pp. 3438-3443. , http://dx.doi.org/10.1016/j.apenergy.2010.12.047Lu, Y., Zhai, Y., Liu, M., Wu, Q., Biodiesel production from algal oil using cassava (Manihot esculenta Crantz) as feedstock (2010) J. Appl. Phycol., 22 (5), pp. 573-578. , http://dx.doi.org/10.1007/s10811-009-9496-8Queiroz, M.I., Hornes, M.O., Manetti, A.G.S., Zepka, L.Q., Jacob-Lopes, E., Fish processing wastewater as a platform of the microalgal biorefineries (2013) Biosyst. Eng., 115 (2), pp. 195-202. , http://dx.doi.org/10.1016/j.biosystemseng.2012.12.013(2013) FAO Statistical Yearbook, , World Food and Agriculture, Food and Agriculture Organization of the United Nations, RomeSubhadra, B.G., Edwards, M., Coproduct market analysis and water footprint of simulated commercial algal biorefineries (2011) Appl. Energy, 88 (10), pp. 3515-3523. , http://dx.doi.org/10.1016/j.apenergy.2010.12.051Shirazi, M.J.A., Bazgir, S., Shirazi, M.M.A., Edible oil mill effluenta low-cost source for economizing biodiesel production: Electrospun nanofibrous coalescing filtration approach (2014) Biofuel Res. J., 1, pp. 39-42Shirazi, M.M., Kargari, A., Tabatabaei, M., Mostafaeid, B., Akia, M., Barkhi, M., Shirazi, M.J., Acceleration of biodiesel-glycerol decantation through NaCl-assisted gravitational settling: A strategy to economize biodiesel production (2013) Bioresour. Technol., 134, pp. 401-406. , http://dx.doi.org/10.1016/j.biortech.2013.02.026.23499494Noureddin, A., Shirazi, M.M.A., Tofeily, J., Kazemi, P., Motaee, E., Kargari, A., Mostafaei, M., Tabatabaei, M., Accelerated decantation of biodiesel-glycerol mixtures: Optimization of a critical stage in biodiesel biorefinery (2014) Sep. Purif. Technol., 132, pp. 272-280. , http://dx.doi.org/10.1016/j.seppur.2014.05.011APhA, AWWA, WEF (Amer. Pub. Hlth. Assoc., Amer. Water Works Assoc., Water Environ. Federation), (2005) Standard Methods for the Examination of Water and Wastewater, Twenty-first Ed., , Prot City Press, Baltimore, MarylandAOAC, (2003) Official Method of Analysis of the Association of Official Agricultural Chemists, 17th Ed., , AOAC International, Gaitherburg, United States of AmericaRippka, R., Deruelles, J., Waterbury, J.B., Herdman, M., Stanier, R.Y., Generic assignments, strain histories and properties of pure cultures of cyanobacteria (1979) J. Gen. Microbiol., 111 (1), pp. 1-61. , http://dx.doi.org/10.1099/00221287-111-1-1(2004) STATISTICA for Windows [Computer Program Manual]., , http://www.statsoftinc.com, [online], StatSoft Inc., Tulsa, OklahomaFrancisco É., C., Franco, T.T., Wagner, R., Jacob-Lopes, E., Assessment of different carbohydrates as exogenous carbon source in cultivation of cyanobacteria (2014) Bioprocess Biosyst. Eng., 37, pp. 1497-1505. , http://dx.doi.org/10.1007/s00449-013-1121-1.24445336Bligh, E.G., Dyer, W.J., A rapid method of total lipid extraction and purification (1959) Can. J. Biochem. Physiol., 37 (8), pp. 911-917. , http://dx.doi.org/10.1139/o59-099Hartman, L., Lago, R.C.A., A rapid determination of fatty acid methyl esters from lipids (1976) Lab. Practice, 22, pp. 475-476Krisnangkura, K.A., Simple method for estimation of cetane index of vegetable oil methyl esters (1986) J. Am. Oil Chem. Soc., 63 (4), pp. 552-553. , http://dx.doi.org/10.1007/BF02645752Ramos, M.J., Fernández, C.M., Casas, A., Rodríguez, L., Pérez, A., Influence of fatty acid composition of raw materials on biodiesel properties (2009) Bioresour. Technol., 100 (1), pp. 261-268. , http://dx.doi.org/10.1016/j.biortech.2008.06.039.18693011Moulijn, J.A., Stankiewicz, A., Grievink, J., Górak, A., Process intensification and process systems engineering: A friendly symbiosis (2008) Comput. Chem. Eng., 32 (1-2), pp. 3-11. , http://dx.doi.org/10.1016/j.compchemeng.2007.05.014Merchuk, J.C., Gluz, M., Mukmenev, I., Comparison of photobioreactors for cultivation of the red microalga Porphyridium sp (2000) J. Chem. Technol. Biotechnol., 75, pp. 1119-1126. , http://dx.doi.org/10.1002/1097-4660(200012)75:12〈1119::AID-JCTB329〉3.3.CO;2-7Xiong, W., Li, X., Xiang, J., Wu, Q., High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production (2008) Appl. Microbiol. Biotechnol., 78, pp. 29-36. , http://dx.doi.org/10.1007/s00253-007-1285-1.18064453Gao, C., Zhai, Y., Ding, Y., Wu, Q., Application of sweet sorghum for biodiesel production by heterotrophic microalga Chlorella protothecoides (2010) Appl. Energy, 87 (3), pp. 756-761. , http://dx.doi.org/10.1016/j.apenergy.2009.09.006Griffiths, M.J., Harrison, S.T.L., Lipid productivity as a key characteristic for choosing algal species for biodiesel production (2009) J. Appl. Phycol., 21 (5), pp. 493-507. , http://dx.doi.org/10.1007/s10811-008-9392-7Francisco É., C., Neves, D.B., Jacob-Lopes, E., Franco, T.T., Microalgae as feedstock for biodiesel production: Carbon dioxide sequestration, lipid production and biofuel quality (2010) J. Chem. Technol. Biotechnol., 85 (3), pp. 395-403. , http://dx.doi.org/10.1002/jctb.2338Standard Specification for biodiesel Fuel (B100) (2002) Blend Stock for Distillate Fuels, , ASTM 6751(2003) Provisional Brazilian Biodiesel Standard ANP (Agência Nacional Do Petróleo), , ANP 255