The study aimed to evaluate the antifungal potential of an extract of propolis obtained with edible vegetable oil (ODEP) and to identify antifungal compounds in this extract. Propolis sample was extracted with canola oil. After filtration, the oily liquid extract was submitted to solvent partition and dried to obtain ODEP, which was tested in vitro against Candida albicans strains. ODEP was fractioned on Sephadex and by high-performance liquid chromatography (HPLC). Active fractions and/or a pure compound were analyzed by nuclear magnetic resonance, liquid chromatographic mass spectrometry (LC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to characterize their chemical composition. Bioassay-guided fractionation allowed the isolation of dihydrokaempferide, which was also quantified in ODEP by HPLC together with the total flavonoid content. Kaempferide and isosakuranetin were identified by LC-MS and LC-MS/MS in fractions with a potential activity against C.albicans. In vitro assays showed that some fractions from ODEP produced better inhibition of C.albicans than the propolis extract itself. © 2013 Wiley Periodicals, Inc.365291301Arabbi, P.R., Genovese, M.I., Lajolo, F.M., Flavonoids in vegetable foods commonly consumed in Brazil and estimated ingestion by the Brazilian population (2004) J. Agric. Food Chem., 52, pp. 1124-1131Banskota, A.H., Tezuka, Y., Prasain, J.K., Matsushige, K., Saiki, I., Kadota, S., Chemical constituents of Brazilian propolis and their cytotoxic activities (1998) J. Nat. Prod., 61, pp. 896-900Bosio, K., Avanzini, C., D'avolio, A., Ozino, O., Savoia, D., In vitro activity of propolis against Streptococcus pyogenes (2000) Lett. Appl. Microbiol., 31, pp. 174-177Buriol, L., Finger, D., Schmidt, E.M., Santos, J.M.T., Rosa, M.R., Quináia, S.P., Torres, Y.R., Moraes, M.O., Chemical composition and biological activity of oil propolis extract: An alternative to ethanolic extract (2009) Quim. Nova, 32, pp. 296-302Cafarchia, C., De Laurentis, N., Milillo, M.A., Losacco, V., Puccini, V., Antifungal activity of Apulia region propolis (1999) Parassitologia, 41, pp. 587-590Carvalho, A.A., Finger, D., Machado, C.S., Schmidt, E.M., Costa, P.M., Alves, A.P.N.N., Morais, T.M.F., Rosa, M.R., In vivo antitumoural activity and composition of an oil extract of Brazilian propolis (2011) Food Chem., 126, pp. 1239-1245Casaroto, A.R., Lara, V.S., Phytomedicines for Candida-associated denture stomatitis (2010) Fitoterapia, 81, pp. 323-328Cizmárik, J., Trupl, J., Propolis-Wirkung auf Hautpilze (1975) Pharmazie, 30, pp. 406-407(2008) Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts, p. 7. , CLinical and Laboratory Standards Institute (CLSI). ., Approved Standard M27-A3, 3rd Ed., CLSI, Wayne, PACuyckens, F., Claeys, M., Mass spectrometry in the structural analysis of flavonoids (2004) J. Mass Spectrom., 39, pp. 1-15D'auria, F.D., Tecca, M., Scazzocchio, F., Renzini, V., Strippoli, V., Effect of propolis on virulence factors of Candida albicans (2003) J. Chemother., 15, pp. 454-460Kujumgiev, A., Tsvetkova, I., Serkedjieva, Y.U., Bankova, V.S., Christov, R., Popov, S., Antibacterial, antifungal and antiviral activity of propolis of different geographic origin (1999) J. Ethnopharmacol., 64, pp. 235-240Lustosa, S.R., Galindo, A.B., Nunes, L.C.C., Randau, K.P., Neto, P.J.R., Própolis: Atualizações sobre a química e farmacologia (2008) Rev. Bras. Farmacogn., 18, pp. 447-454Ma, Y.L., Li, Q.M., Van Den Heuvel, H., Claeys, M., Characterization of flavone and flavonol aglycones by collision-induced dissociation tandem mass spectrometry (1997) Rapid Commun. Mass Spectrom., 11, pp. 1357-1364Malterud, K.E., Bremnes, T.E., Faegri, A., Moe, T., Dugstad, E.K.S., Flavonoids from the wood of Salix caprea as inhibitors of wood-destroying fungi (1985) J. Nat. Prod., 48, pp. 559-563(2001), Ministério da Agricultura e do Abastecimento Secretaria de Defesa Agropecuária. Instrução Normativa N°3 BrasíliaOta, C., Unterkircher, C., Fantinato, V., Shimiziu, M.T., Antifungal activity of propolis on different species of Candida (2001) Mycoses, 44, pp. 375-378Pepeljnjak, S., Kosalec, I., Galangin express bactericidal activity against multiple-resistant bacteria: MRSA, Enterococcus spp. and Pseudomonas aeruginosa (2004) FEMS Microbiol. Lett., 240, pp. 111-116Quadros, A.U., Bini, D., Pereira, P.A.T., Moroni, E.G., Monteiro, M.C., Antifungal activity of some cyclooxygenase inhibitors on Candida albicans: PGE2-dependent mechanism (2011) Folia Microbiol. (Dordrecht, Neth.), 56, pp. 349-352Ribani, M., Bottoli, C.B.G., Collins, C.H., Jardim, I.C.S.F., Melo, L.F.C., Validação em métodos cromatográficos e eletroforéticos (2004) Quim. Nova, 27, pp. 771-780Rojas, A., Hernandez, L., Pereda-Miranda, R., Mata, R., Screening for antimicrobial activity of crude drug extracts and pure natural products from Mexican medicinal plants (1992) J. Ethnopharmacol., 35, pp. 275-283Salomão, K., Dantas, A.P., Borba, C.M., Campos, L.C., Machado, D.G., Aquino Neto, F.R., De Castro, S.L., Chemical composition and microbicidal activity of extracts from Brazilian and Bulgarian propolis (2004) Lett. Appl. Microbiol., 38, pp. 87-92Sawaya, A.C.H.F., Palma, A.M., Caetano, F.M., Marcucci, M.C., Cunha, I.B.S., Araujo, C.E.P., Shimizu, M.T., Comparative study of in vitro methods used to analyse the activity of propolis extracts with different compositions against species of Candida (2002) Lett. Appl. Microbiol., 35, pp. 203-207Sawaya, A.C.H.F., Souza, K.S., Marcucci, M.C., Cunha, I.B.S., Shimizu, M.T., Analysis of the composition of Brazilian propolis extracts by chromatography and evaluation of their in vitro activity against gram-positive bacteria (2004) Braz. J. Microbiol., 35, pp. 104-109Sawaya, A.C.H.F., Tomazela, D.M., Cunha, I.B.S., Bankova, V.S., Marcucci, M.C., Custodio, A.R., Eberlin, M.N., Electrospray ionization mass spectrometry fingerprint of propolis (2004) Analyst, 129, pp. 739-744Sawaya, A.C.H.F., Cunha, I.B.S., Marcucci, M.C., Analytical methods applied to diverse types of Brazilian propolis (2011) Chem. Cent. J., 5, pp. 27-37Sforcin, J.M., Fernandes Jr, A., Lopes, C.A.M., Funari, S.R.C., Bankova, V., Seasonal effect of Brazilian propolis on Candida albicans and Candida tropicalis (2001) J. Venom. Anim. Toxins., 7, pp. 139-144Sforcin, J.M., Bankova, V., Propolis: Is there a potential for the development of new drugs? (2011) J. Ethnopharmacol., 133, pp. 253-260Shokri, H., Khosravi, A.R., Yalfani, R., Antifungal efficacy of propolis against fluconazole-resistant Candida glabrata isolates obtained from women with recurrent vulvovaginal candidiasis (2011) Int. J. Gynecol. Obstet., 114, pp. 58-159Silici, S., Kutluca, S., Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region (2005) J. Ethnopharmacol., 13, pp. 69-73Stepanović, S., Antić, N., Dakić, I., Švabić-Vlahović, M., In vitro antimicrobial activity of propolis and synergism between propolis and antimicrobial drugs (2003) Microbiol. Res., 158, pp. 353-357Tosi, B., Donini, A., Romagnoli, C., Bruni, A., Antimicrobial activity of some commercial extracts of propolis prepared with different solvents (1996) Phytother. Res., 10, pp. 335-336Uzel, A., Sorkun, K., Önçaǧ, O., Çoǧulu, D., Gençay, O., Salih, B., Chemical compositions and antimicrobial activities of four different Anatolian propolis samples (2005) Microbiol. Res., 160, pp. 189-195Van Reensburg, H., Van Heerden, P.S., Bezuidenhoudt, B.C.B., Ferreira, D., Stereoselective synthesis of flavonoids. Part 4. 1,2 Trans- and cis- dihydroflavonols (1997) Tetrahedron, 53, pp. 14141-14152Yang, S.Z., Peng, L.T., Su, X.J., Chen, F., Cheng, Y.J., Fan, G., Pan, S.Y., Bioassay-guided isolation and identification of antifungal components from propolis against Penicillium italicum (2011) Food Chem., 127, pp. 210-215