Modulation Of Mitochondrial Activity By S-nitrosoglutathione Reductase In Arabidopsis Thaliana Transgenic Cell Lines

dc.creatorFrungillo L.
dc.creatorDe Oliveira J.F.P.
dc.creatorSaviani E.E.
dc.creatorOliveira H.C.
dc.creatorMartinez M.C.
dc.creatorSalgado I.
dc.date2013
dc.date2015-06-25T19:15:24Z
dc.date2015-11-26T15:13:05Z
dc.date2015-06-25T19:15:24Z
dc.date2015-11-26T15:13:05Z
dc.date.accessioned2018-03-28T22:23:10Z
dc.date.available2018-03-28T22:23:10Z
dc.identifier
dc.identifierBiochimica Et Biophysica Acta - Bioenergetics. , v. 1827, n. 3, p. 239 - 247, 2013.
dc.identifier52728
dc.identifier10.1016/j.bbabio.2012.11.011
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84871987593&partnerID=40&md5=0eda499765c96ae57fec35a2a1860d93
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/89245
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/89245
dc.identifier2-s2.0-84871987593
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1258547
dc.descriptionThe enzyme S-nitrosoglutathione reductase (GSNOR) has an important role in the metabolism of S-nitrosothiols (SNO) and, consequently, in the modulation of nitric oxide (NO)-mediated processes. Although the mitochondrial electron transport chain is an important target of NO, the role of GSNOR in the functionality of plant mitochondria has not been addressed. Here, we measured SNO content and NO emission in Arabidopsis thaliana cell suspension cultures of wild-type (WT) and GSNOR overexpressing (GSNOROE) or antisense (GSNORAS) transgenic lines, grown under optimal conditions and under nutritional stress. Respiratory activity of isolated mitochondria and expression of genes encoding for mitochondrial proteins were also analyzed. Under optimal growth conditions, GSNOROE had the lowest SNO and NO levels and GSNORAS the highest, as expected by the GSNO-consuming activity of GSNOR. Under stress, this pattern was reversed. Analysis of oxygen uptake by isolated mitochondria showed that complex I and external NADH dehydrogenase activities were inhibited in GSNOROE cells grown under nutritional stress. Moreover, GSNOROE could not increase alternative oxidase (AOX) activity under nutritional stress. GSNORAS showed constitutively high activity of external NADH dehydrogenase, and maintained the activity of the uncoupling protein (UCP) under stress. The alterations observed in mitochondrial protein activities were not strictly correlated to changes in gene expression, but instead seemed to be related with the SNO/NO content, suggesting a post-transcriptional regulation. Overall, our results highlight the importance of GSNOR in modulating SNO and NO homeostasis as well mitochondrial functionality, both under normal and adverse conditions in A. thaliana cells. © 2012 Elsevier B.V.
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dc.languageen
dc.publisher
dc.relationBiochimica et Biophysica Acta - Bioenergetics
dc.rightsfechado
dc.sourceScopus
dc.titleModulation Of Mitochondrial Activity By S-nitrosoglutathione Reductase In Arabidopsis Thaliana Transgenic Cell Lines
dc.typeArtículos de revistas


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