Artículos de revistas
Mitochondrial Swelling And Oxygen Consumption During Respiratory State 4 Induced By Phospholipase A2 Isoforms Isolated From The South American Rattlesnake (crotalus Durissus Terrificus) Venom
Registro en:
Toxicon. , v. 36, n. 6, p. 901 - 913, 1998.
410101
10.1016/S0041-0101(97)00107-4
2-s2.0-0031832560
Autor
Valente R.H.
Novello J.C.
Marangoni S.
Oliveira B.
Pereira-Da-Silva L.
MacEdo D.V.
Institución
Resumen
The non-covalent interaction between two molecular entities namely, phospholipase A2 and crotapotin, results in the main toxin, crotoxin, present in the venom of the South American rattlesnake Crotalus durissus terrificus. High performance liquid chromatography has enabled us the isolation of three phospholipase A2 isoforms (F1, F2 and F3), characterized through denaturing and non-denaturing polyacrylamide gel electrophoresis and also through the N-terminal amino acid sequence analysis. The effect of each purified phospholipase A2 isoform on isolated rat liver mitochondria was determined through mitochondrial swelling and O2 consumption during respiratory state 4. F1 showed a dose-dependent stimulation of O2 consumption while F2 and F3 caused stimulation only at low doses and inhibition at high amounts. These effects were completely suppressed by the presence of 0.1% bovine serum albumin or 0.5 mM EGTA in the incubation medium. 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