Neuropathic pain is characterized to arise without stimulation of nociceptors, but due to injury or dysfunction of Peripheral and Central Nervous Systems. It involves altered mechanisms of impulse transmission in somatosensory pathways, causing abnormal sensations. Quantitative sensory testing, by the detection of thermal stimuli, is a method used to characterize and study the neuropathic pain. Therefore, this work describes the development and application of portable custom built device for cutaneous thermal sensitivity assessment in spinal cord injured subjects (SCIS). Using method of levels, the assessment was applied in healthy subjects and SCIS with and without neuropathic pain. The thresholds determined for healthy subjects during thermal sensitivity assessment are consistent and other results provided by clinical trials are according to previous works, demonstrating the device feasibility as an auxiliary tool for neuropathic pain study. 2834 Inst. Syst. Technol. 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