Objectives: Several medical and dental schools have described their experience in the transition from conventional to digital microscopy in the teaching of general pathology and histology disciplines; however, this transitional process has scarcely been reported in the teaching of oral pathology. Therefore, the objective of the current study is to report the transition from conventional glass slide to virtual microscopy in oral pathology teaching, a unique experience in Latin America.Study Design: An Aperio ScanScope® scanner was used to digitalize histological slides used in practical lectures of oral pathology. The challenges and benefts observed by the group of Professors from the Piracicaba Dental School (Brazil) are described and a questionnaire to evaluate the students’ compliance to this new methodology was applied.Results: An improvement in the classes was described by the Professors who mainly dealt with questions related to pathological changes instead of technical problems; also, a higher interaction with the students was described. The simplicity of the software used and the high quality of the virtual slides, requiring a smaller time to identify microscopic structures, were considered important for a better teaching process.Conclusions: Virtual microscopy used to teach oral pathology represents a useful educational methodology, with an excellent compliance of the dental students.201e17e22Coleman, R., Can histology and pathology be taught without microscopes? The advantages and disadvantages of virtual histology (2009) Acta Histochem, 111, pp. 1-4Rocha, R., Vassallo, J., Soares, F., Miller, K., Gobbi, H., Digital slides: Present status of a tool for consultation, teaching, and quality control in pathology (2009) Pathol Res Pract, 205, pp. 735-741Taylor, C.R., From microscopy to whole slide digital images: A century and a half of image analysis (2011) Appl Immunohistochem Mol Morphol, 19, pp. 491-493Dee, F.R., Virtual microscopy in pathology education (2009) Hum Pathol, 40, pp. 1112-1121Szymas, J., Lundin, M., Five years of experience teaching pathology to dental students using the WebMicroscope (2011) Diag Pathol, 6, p. S13Hamilton, P.W., Wang, Y., McCullough, S.J., Virtual microscopy and digital pathology in training and education (2012) APMIS, 120, pp. 305-315Harris, T., Leaven, T., Heidger, P., Kreiter, C., Duncan, J., Dick, F., Comparison of a virtual microscope laboratory to a regular microscope laboratory for teaching histology (2001) Anat Rec, 265, pp. 10-14Blake, C.A., Lavoie, H.A., Millette, C.F., Teaching medical histology at the University of South Carolina School of Medicine: Transition to virtual slides and virtual microscopes (2003) Anat Rec, 275, pp. 196-206Chen, Y.K., Hsue, S.S., Lin, D.C., Wang, W.C., Chen, J.Y., Lin, C.C., An application of virtual microscopy in the teaching of an oral and maxillofacial pathology laboratory course (2008) Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 105, pp. 342-347Farah, C.S., Maybury, T.S., The e-evolution of microscopy in dental education (2009) J Dent Educ, 73, pp. 942-949Weaker, F.J., Herbert, D.C., Transition of a dental histology course from light to virtual microscopy (2009) J Dent Educ, 73, pp. 1213-1221Schmidt, P., Digital learning programs -competition for the classical microscope? (2013) GMS Z Med Ausbild, 30, p. 8Rosas, C., Rubí, R., Donoso, M., Uribe, S., Dental students’ evaluations of an interactive histology software (2012) J Dental Educ, 76, pp. 1491-1496