Human Nek7-interactor Rgs2 Is Required For Mitotic Spindle Organization

dc.creatorDe Souza E.E.
dc.creatorHehnly H.
dc.creatorPerez A.M.
dc.creatorMeirelles G.V.
dc.creatorSmetana J.H.C.
dc.creatorDoxsey S.
dc.creatorKobarg J.
dc.date2015
dc.date2015-06-25T12:53:23Z
dc.date2015-11-26T15:09:23Z
dc.date2015-06-25T12:53:23Z
dc.date2015-11-26T15:09:23Z
dc.date.accessioned2018-03-28T22:19:36Z
dc.date.available2018-03-28T22:19:36Z
dc.identifier
dc.identifierCell Cycle. Landes Bioscience, v. 14, n. 4, p. 656 - 667, 2015.
dc.identifier15384101
dc.identifier10.4161/15384101.2014.994988
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84923529880&partnerID=40&md5=a6c0891ce06098aa4b64ea15bf2dfc94
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/85466
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/85466
dc.identifier2-s2.0-84923529880
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1257809
dc.descriptionThe mitotic spindle apparatus is composed of microtubule (MT) networks attached to kinetochores organized from 2 centrosomes (a.k.a. spindle poles). In addition to this central spindle apparatus, astral MTs assemble at the mitotic spindle pole and attach to the cell cortex to ensure appropriate spindle orientation. We propose that cell cycle-related kinase, Nek7, and its novel interacting protein RGS2, are involved in mitosis regulation and spindle formation. We found that RGS2 localizes to the mitotic spindle in a Nek7-dependent manner, and along with Nek7 contributes to spindle morphology and mitotic spindle pole integrity. RGS2-depletion leads to a mitotic-delay and severe defects in the chromosomes alignment and congression. Importantly, RGS2 or Nek7 depletion or even overexpression of wild-type or kinase-dead Nek7, reduced γ-tubulin from the mitotic spindle poles. In addition to causing a mitotic delay, RGS2 depletion induced mitotic spindle misorientation coinciding with astral MT-reduction. We propose that these phenotypes directly contribute to a failure in mitotic spindle alignment to the substratum. In conclusion, we suggest a molecular mechanism whereupon Nek7 and RGS2 may act cooperatively to ensure proper mitotic spindle organization.
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dc.languageen
dc.publisherLandes Bioscience
dc.relationCell Cycle
dc.rightsfechado
dc.sourceScopus
dc.titleHuman Nek7-interactor Rgs2 Is Required For Mitotic Spindle Organization
dc.typeArtículos de revistas


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