The Amazonian tree Aniba rosaeodora Ducke, which provides a valued essential oil for perfume industry, is at risk of extinction. An alternative source of this product would be the oil obtained by steam distillation of the leaves of the same plant-which does not involve sacrifice of the tree. However, there is still not a technical criterion to ensure that determined oil is result of a sustainable production process. One step towards defining to know the differences in the oil compositions of trees of different stages of growth to establish the age from which the tree can be commercially explored and to differentiate products obtained from cultivated young plants and from native trees. In this paper, the characterization an differentiation of the essential oil extracted from the leaves collected from trees with different ages (4, 10 and 20. years old) was performed by Comprehensive Two-Dimensional Gas Chromatography coupled with Quadrupole Mass Spectrometric detection (GC. ×. GC-qMS). GC. ×. GC-qMS allowed the identification of ca. three times more chemical compounds on these samples, when compared to conventional gas chromatography. Depending on the age of the tree used to produce the oil, few differences in minor constituents of the oil samples were found; the amounts of the major compounds are similar all samples. Reliable differentiation of the essential oils according to the age of the source was only possible by GC. ×. GC-qMS. © 2012 Elsevier B.V.109 7377Maia, J.G.S., Andrade, E.H.A., Couto, H.A.R., da Silva, A.C., Marx, F., Henke, C., Plant sources of Amazon rosewood oil (2007) Quim. Nova, 30, pp. 906-1910Lupe, F., Souza, R., Barata, L.E.S., Seeking a sustainable alternative to Brazilian rosewood (2008) Perfum. Flavor., 33, pp. 40-43Chantraine, J.-M., Dhénin, J.-M., Moretti, C., Chemical variability of rosewood (Aniba rosaeodora Ducke) essential oil in French Guiana (2009) J. Ess. Oil Res., 21, pp. 486-495Barata, L.E.S., de Carvalho, R.Q., Amazon scents: replacing rosewood in perfumery? (2007) Part I. Perfum. Flav., 32. , http://www.perfumerflavorist.com/fragrance/rawmaterials/natural/6599747.html, Available at [Accessed on 17/04/2012]Barata, L.E.S., de Carvalho, R.Q., Amazon scents: replacing rosewood in perfumery? (2007) Part II. Perfum. Flavor., 32. , http://www.perfumerflavorist.com/fragrance/rawmaterials/natural/6845957.html, Available at [Accessed on 17/04/2012]Barata, L.E.S., May, P., Rosewood exploitation in the Brazilian Amazon: options for sustainable production (2004) Econ. Bot., 58, pp. 257-265Dunford, N.T., Hiziroglu, S., Holcomb, R., Effect of age on the distribution of oil in Eastern redcedar tree segments (2007) Bioresour. Technol., 98, pp. 2636-2640Kim, J.C., Kim, K.J., Kim, D.S., Han, J.S., Seasonal variations of monoterpene emissions from coniferous trees of different ages in Korea (2005) Chemosphere, 59, pp. 1685-1696Geng, S., Cui, Z., Huang, X., Chen, Y., Xu, D., Xiong, P., Variations in essential oil yield and composition during Cinnamomum cassia bark growth (2011) Ind. Crops Prod., 33, pp. 248-252Cheng, S.-S., Chua, M.-T., Chang, E.-H., Huang, C.-G., Chen, W.-J., Chang, S.-T., Variations in insecticidal activity and chemical compositions of leaf essential oils from Cryptomeria japonica at different ages (2009) Bioresour. Technol., 100, pp. 465-470Mondello, L., Tranchida, P.Q., Dugo, P., Dugo, G., Comprehenseive two-dimensional gas chromatography-mass spectrometry: a review (2008) Mass Spectrom. Rev., 27, pp. 101-124Pripdeevech, P., Wongpornehai, S., Promsiri, A., Highly volatile constituents of Vetiveria zizanioides roots grown under different cultivation conditions (2006) Molecules, 11, pp. 817-826Shellie, R.A., Morrison, P.D., Marriott, P.J., Poynter, S.D.H., Selection of columns for GC×GC analysis of essential oils (2010) LC GC Europe, 23, pp. 76-80Tranchida, P.Q., Shellie, R.A., Purcaro, G., Conte, L.S., Dugo, P., Dugo, G., Mondello, L., Analysis of fresh and aged tea tree essential oils by using GC×GC-qMS (2010) J. Chromatogr. Sci., 48, pp. 262-266Kutlular, O., Ozel, M.Z., Analysis of essential oils of origanum onites by superheated water extraction using GC×GC-TOF/MS (2009) J. Essent. Oil Bear. Plants, 12, pp. 462-470Fidelis, C.H.V., Augusto, F., Sampaio, P.T.B., Krainovic, P.M., Barata, L.E.S., Chemical characterization of rosewood (Aniba rosaeodora Ducke) leaf essential oil by comprehensive two-dimensional gas chromatography coupled with quadrupole mass spectrometry (2012) J. Ess. Oil. Res, 24, pp. 245-251Tranchida, P.Q., de Souza, R.C.Z., Barata, L.E.S., Mondello, M., Dugo, P., Dugo, G., Mondello, L., Analysis of Macacaporanga (Aniba parviflora) leaf essential oil by using comprehensive two-dimensional gas chromatography combined with rapid-scanning quadrupole mass spectrometry (2008) Chromatogr. Today, pp. 5-9de Godoy, L.A.F., Ferreira, E.C., Pedroso, M.P., Fidelis, C.H.V., Augusto, F., Poppi, R.J., Quantification of kerosene in gasoline by comprehensive two-dimensional gas chromatography and N-way multivariate analysis (2008) Anal. Lett., 41, pp. 1603-1614Shellie, R.A., Marriott, P.J., Comprehensive two-dimensional gas chromatography-mass spectrometry analysis of Pelargonium graveolens essential oil using rapid scanning quadrupolar mass spectrometry (2003) Analyst, 128, pp. 879-883Bieri, S., Marriott, P.J., Generating multiple independent retention index data in dual-secondary column comprehensive two-dimensional gas chromatography (2006) Anal. Chem., 78, pp. 8089-8097von Mühlen, C., Zini, C.A., Caramão, E.B., Marriott, P.J., Comparative study of Eucalyptus dunnii volatile oil composition using retention indices and comprehensive two-dimensional gas chromatography coupled to time-of-flight and quadrupolar mass spectrometry (2008) J. Chromatogr. A, 1200, pp. 34-42Pedroso, M.P., De Godoy, L.A.F., Ferreira, E.C., Poppi, R.J., Augusto, F., Identification of gasoline adulteration using comprehensive two-dimensional gas chromatography combined to multivariate data processing (2008) J. Chromatogr. A, 1201, pp. 176-182de Godoy, L.A.F., Hantao, L.W., Pedroso, M.P., Poppi, R.J., Augusto, F., Quantitative analysis of essential oils in perfume using multivariate curve resolution combined with comprehensive two-dimensional gas chromatography (2011) Anal. Chim. Acta, 699, pp. 120-125de Godoy, L.A.F., Pedroso, M.P., Hantao, L.W., Poppi, R.J., Augusto, F., Quantitative analysis by comprehensive two-dimensional gas chromatography using interval Multi-way Partial Least Squares calibration (2011) Talanta, 83, pp. 1302-1307de Godoy, L.A.F., Pedroso, M.P., Ferreira, E.C., Augusto, F., Poppi, R.J., Prediction of the physicochemical properties of gasoline by comprehensive two-dimensional gas chromatography and multivariate data processing (2011) J. Chromatogr. A, 1218, pp. 1663-1667Pedroso, M.P., Ferreira, E.C., Hantao, L.W., Bogusz, S., Augusto, F., Identification of volatiles from pineapple (Ananas comosus L.) pulp by comprehensive two-dimensional gas chromatography and gas chromatography/mass spectrometry (2011) J. Sep. Sci., 34, pp. 1547-1554NIST Chemistry WebBook, , http://webbook.nist.gov/chemistry, National Institute of Standards and TechnologyAdams, R.P., (2007) Identification of Essential Oil Components by Gas Chromatography/Quadrupole Mass Spectroscopy, , Allured Publ, Corp, Carol Stream, ILvan den Dool, H., Kratz, P.D., A generalization of the retention index system including linear temperature programmed gas-liquid partition chromatography (1963) J. Chromatogr. A, 11, pp. 463-471d'A.Zellner, B., Lo Presti, M., Barata, L.E.S., Dugo, P., Dugo, G., Mondello, L., Evaluation of leaf-derived extracts as an environmentally sustainable source of essential oils by using gas chromatography-mass spectrometry and enantioselective gas chromatography-olfatometry (2006) Anal. Chem., 78, pp. 883-890Sell, C., Hüsnü, K., (2010), pp. 121-149. , Chemistry of Essential Oils, in: Can Baser and Gerhard Buchbauer (Eds.), Handbook of Essential Oils Science, Technology, and Applications, CRC Press. Print ISBN: 978-1-4200-6315-8eBook ISBN: 978-1-4200-6316-5