Enhanced Peripheral Nerve Regeneration By The Combination Of A Polycaprolactone Tubular Prosthesis And A Scaffold Of Collagen With Supramolecular Organization

dc.creatorMaturana L.G.
dc.creatorPierucci A.
dc.creatorSimoes G.F.
dc.creatorVidigal M.
dc.creatorDuek E.A.R.
dc.creatorVidal B.C.
dc.creatorOliveira A.L.R.
dc.date2013
dc.date2015-06-25T19:10:56Z
dc.date2015-11-26T15:08:27Z
dc.date2015-06-25T19:10:56Z
dc.date2015-11-26T15:08:27Z
dc.date.accessioned2018-03-28T22:18:50Z
dc.date.available2018-03-28T22:18:50Z
dc.identifier
dc.identifierBrain And Behavior. , v. 3, n. 4, p. 417 - 430, 2013.
dc.identifier21623279
dc.identifier10.1002/brb3.145
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84893475220&partnerID=40&md5=3b4641316f37dd45494e77b190b585d0
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/88574
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/88574
dc.identifier2-s2.0-84893475220
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1257664
dc.descriptionThe purpose of this study was to investigate the influence of implanting collagen with a supramolecular organization on peripheral nerve regeneration, using the sciatic nerve tubulization technique. For this purpose, adult female Sprague Dawley rats were divided into five groups: (1) TP - sciatic nerve repaired with empty polyethylene tubular prothesis (n = 10), (2) TPCL - nerve repair with empty polycaprolactone (PCL) tubing (n = 8), (3) TPCLF - repair with PCL tubing filled with an implant of collagen with a supramolecular organization (n = 10), (4) AG - animals that received a peripheral nerve autograft (n = 8), and (5) Normal nerves (n = 8). The results were assessed by quantification of the regenerated fibers, nerve morphometry, and transmission electron microscopy, 60 days after surgery. Immunohistochemistry and polarization microscopy were also used to analyze the regenerated nerve structure and cellular elements. The results showed that the AG group presented a larger number of regenerated axons. However, the TPCL and TPCLF groups presented more compact regenerated fibers with a morphometric profile closer to normal, both at the tube midpoint and 2 mm distal to the prosthesis. These findings were reinforced by polarization microscopy, which indicated a better collagen/axons suprastructural organization in the TPCLF derived samples. In addition, the immunohistochemical results obtained using the antibody anti-p75NTR as a Schwann cell reactivity marker demonstrated that the Schwann cells were more reactive during the regenerative process in the TPCLF group as compared to the TPCL group and the normal sciatic nerve. Altogether, the results of this study indicated that the implant of collagen with a supramolecular organization positively influenced and stimulated the regeneration process through the nerve gap, resulting in the formation of a better morphologically arranged tissue. © 2013 The Authors. Brain and Behavior published by Wiley Periodicals, Inc.
dc.description3
dc.description4
dc.description417
dc.description430
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dc.languageen
dc.publisher
dc.relationBrain and Behavior
dc.rightsfechado
dc.sourceScopus
dc.titleEnhanced Peripheral Nerve Regeneration By The Combination Of A Polycaprolactone Tubular Prosthesis And A Scaffold Of Collagen With Supramolecular Organization
dc.typeArtículos de revistas


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