Artículos de revistas
Partial Recovery Of Erythrocyte Glycogen In Diabetic Rats Treated With Phenobarbital
Registro en:
Brazilian Journal Of Medical And Biological Research. , v. 30, n. 5, p. 657 - 661, 1997.
0100879X
2-s2.0-0030912953
Autor
Da-Silva C.A.
Goncalves A.A.
Institución
Resumen
Erythrocytes may play a role in glucose homeostasis during the postprandial period. Erythrocytes from diabetic patients are defective in glucose transport and metabolism, functions that may affect glycogen storage. Phenobarbital, a hepatic enzyme inducer, has been used in the treatment of patients with non-insulin-dependent diabetes mellitus (NIDDM), increasing the insulin-mediated glucose disposal. We studied the effects of phenobarbital treatment in vivo on glycemia and erythrocyte glycogen content in control and alloxan-diabetic rats during the postprandial period. In control rats (blood glucose, 73 to 111 mg/dl in femoral and suprahepatic veins) the erythrocyte glycogen content was 45.4 ± 1.1 and 39.1 ± 0.8 μg/g Hb (mean ± SEM, N = 4-6) in the femoral artery and vein, respectively, and 37.9 ± 1.1 in the portal vein and 47.5 ± 0.9 in the suprahepatic vein. Diabetic rats (blood glucose, 300-350 mg/dl) presented low (P < 0.05) erythrocyte glycogen content, i.e., 9.6 ± 0.1 and 7.1 ± 0.7 μg/g Hb in the femoral artery and vein, respectively, and 10.0 ± 0.7 and 10.7 ± 0.5 in the portal and suprahepatic veins, respectively. After 10 days of treatment, phenobarbital (0.5 mg/ml in the drinking water) did not change blood glucose or erythrocyte glycogen content in control rats. In diabetic rats, however, it lowered (P < 0.05) blood glucose in the femoral artery (from 305 ± 18 to 204 ± 45 mg/dl) and femoral vein (from 300 ± 11 to 174 ± 48 mg/dl) and suprahepatic vein (from 350 ± 10 to 174 ± 42 mg/dl), but the reduction was not sufficient for complete recovery. Phenobarbital also stimulated the glycogen synthesis, leading to a partial recovery of glycogen stores in erythrocytes. In treated rats, erythrocyte glycogen content increased to 20.7 ± 3.8 μg/g Hb in the femoral artery and 30.9 ± 0.9 μg/g Hb in the suprahepatic vein (P < 0.05). These data indicate that phenobarbital activated some of the insulin-stimulated glucose metabolism steps which were depressed in diabetic erythrocytes, supporting the view that erythrocytes participate in glucose homeostasis. 30 5 657 661 Burant, C.F., Sivitz, W.I., Fukumoto, H., Kayano, T., Nagamatsu, S., Seino, S., Pessin, J.E., Bell, G.I., Mammalian glucose transporters: Structure and molecular regulation (1991) Recent Progress in Hormone Research, 47, pp. 1-41 Zoccoli, M.A., Boldwin, S.A., Lienhard, G.E., Monosaccharide transport system of the human erythrocyte (1978) Journal of Biological Chemistry, 253, pp. 69213-69230 Rapoport, S., The regulation of glycolysis in mammalian erythrocytes (1968) Essays in Biochemistry, 4, pp. 69-103 Jacquez, J.A., Red blood cell as glucose carrier: Significance for placental and cerebral glucose transfer (1984) American Journal of Physiology, 246, pp. 289-298 Ferranini, E., Bjorkman, O., Role of red blood cells in the regulation of blood glucose levels in man (1986) Diabetes, 35 (1 SUPPL.), p. 39. , Abstract Guarner, V., Alvarez-Buylla, R., Erythrocyte and glucose homeostasis in rats (1989) Diabetes, 38, pp. 410-415 Hers, H.G., Verhue, W., Van Hoof, F., The determination of amylo-1,6-glucosidase (1967) European Journal of Biochemistry, 2, pp. 256-264 Moses, S.W., Bashan, N., Gutman, A., Properties of glycogen synthetase in erythrocytes (1972) European Journal of Biochemistry, 30, pp. 205-210 Garvey, W.T., Hueckstedt, T.P., Olefsky, J.M., Glucose and insulin co-regulate the glucose transport system in primary cultured adipocytes. A new mechanism of insulin resistance (1987) Diabetes, 36 (1 SUPPL.), p. 84. , Abstract Rapin, J.R., Lespinasse, C., Yoa, R., Wiernsperger, N., Erythrocyte glucose consumption in insulin-dependent diabetes: Effect of metformin in vitro (1991) Diabete et Metabolisme, 14, pp. 164-167 Lahtela, J.T., Sarkka, P., Sotaniemi, E.A., Phenobarbital treatment enhances insulin mediated glucose metabolism in man' (1984) Research Communications in Chemical Pathology and Pharmacology, 44, pp. 215-226 Lahtela, J.T., Gachalyi, B., Eksyma, S., The effect of liver microsomal enzyme inducing and inhibiting drugs on insulin mediated glucose metabolism in man (1986) British Journal of Clinical Pharmacology, 21, pp. 19-26 Lahtela, J.T., Arranto, A.J., Sotaniemi, E.A., Enzyme inducers improve insulin sensitivity in non-insulin-dependent diabetic subjects (1985) Diabetes, 34, pp. 911-916 Farquharson, J., Jamieson, E.C., MacPhee, G.B., Logan, R.W., A new sensitive microassay for the measurement of erythrocyte glycogen (1990) Clinica Chimica Acta, 187, pp. 89-94 Venkatsen, N., Davidson, M.B., Simsolo, R.B., Kern, P.A., Phenobarbital treatment enhances insulin-mediated glucose metabolism and improves lipid metabolism in the diabetic rat (1994) Metabolism: Clinical and Experimental, 43, pp. 348-356 Thurman, R.G., Kauffman, F.C., Factors regulating drug metabolism in the intact hepatocytes (1980) Pharmacological Reviews, 31, pp. 229-251 Karvonen, I., Stengard, J.H., Huupponen, R., Effects of enzyme induction therapy on glucose and drug metabolism in obese mice model of non-insulin dependent diabetes mellitus (1989) Diabetes Research, 10, pp. 85-92 Villar-Palasi, C., Effect of glucose phosphorylation on the activation by insulin of skeletal muscle glycogen synthase (1995) Biochimica et Biophysica Acta, 1244, pp. 203-208