Extracts from malagueta pepper (Capsicum frutescens L.) were obtained using supercritical fluid extraction (SFE) assisted by ultrasound, with carbon dioxide as solvent at 15 MPa and 40 °C. The SFE global yield increased up to 77% when ultrasound waves were applied, and the best condition of ultrasound-assisted extraction was ultrasound power of 360 W applied during 60 min. Four capsaicinoids were identified in the extracts and quantified by high performance liquid chromatography. The use of ultrasonic waves did not influence significantly the capsaicinoid profiles and the phenolic content of the extracts. However, ultrasound has enhanced the SFE rate. A model based on the broken and intact cell concept was adequate to represent the extraction kinetics and estimate the mass transfer coefficients, which were increased with ultrasound. Images obtained by field emission scanning electron microscopy showed that the action of ultrasonic waves did not cause cracks on the cell wall surface. On the other hand, ultrasound promoted disturbances in the vegetable matrix, leading to the release of extractable material on the solid surface. The effects of ultrasound were more significant on SFE from larger solid particles. © 2014 Elsevier B.V. All rights reserved.22 7888Laskaridou-Monnerville, A., Determination of capsaicin and dihydrocapsaicin by micellar electrokinetic capillary chromatography and its application to various species of Capsicum, Solanaceae (1999) J. Chromatogr. A, 838, pp. 293-302Damodaran, S., Parkin, K.L., Fennema, O.R., (2008) Fennema's Food Chemistry, , CRC Press/Taylor & Francis Boca RatonReyes-Escogido, M., Gonzalez-Mondragon, E.G., Vazquez-Tzompantzi, E., Chemical and pharmacological aspects of capsaicin (2011) Molecules, 16, pp. 1253-1270De Aguiar, A.C., Sales, L.P., Coutinho, J.P., Barbero, G.F., Godoy, H.T., Martínez, J., Supercritical carbon dioxide extraction of capsicum peppers: Global yield and capsaicinoid content (2013) J. Supercrit. Fluids, , 10.1016/j.supflu.2013.05.008Martinez, J.L., (2008) Supercritical Fluid Extraction of Nutraceuticals and Bioactive Compounds, , CRC Press Boca Raton-FLBrunner, G., (1994) Gas Extraction: An Introduction to Fundamentals of Supercritical Fluids and the Application to Separation Processes, , SteinkopffSpringer ed., Darmstadt New YorkBrunner, G., Supercritical fluids: Technology and application to food processing (2005) J. Food Eng., 67, pp. 21-33Perva-Uzunalić, A., Škerget, M., Weinreich, B., Knez, Ž., Extraction of chilli pepper (var. Byedige) with supercritical CO 2: Effect of pressure and temperature on capsaicinoid and colour extraction efficiency (2004) Food Chem., 87, pp. 51-58Zabot, G.L., Moraes, M.N., Meireles, M.A.A., Supercritical fluid extraction of bioactive compounds from botanic matrices: Experimental data, process parameters and economic evaluation (2012) Recent Pat. Eng., 6, pp. 182-206Esclapez, M.D., García-Pérez, J.V., Mulet, A., Cárcel, J.A., Ultrasound-assisted extraction of natural products (2011) Food Eng. Rev., 3, pp. 108-120Gao, Y., Nagy, B., Liu, X., Simándi, B., Wang, Q., Supercritical CO2 extraction of lutein esters from marigold (Tagetes erecta L.) enhanced by ultrasound (2009) J. Supercrit. Fluids, 49, pp. 345-350Toma, M., Vinatoru, M., Paniwnyk, L., Mason, T.J., Investigation of the effects of ultrasound on vegetal tissues during solvent extraction (2001) Ultrason. Sonochem., 8, pp. 137-142Carrera, C., Ruiz-Rodríguez, A., Palma, M., Barroso, C.G., Ultrasound assisted extraction of phenolic compounds from grapes (2012) Anal. Chim. Acta, 732, pp. 100-104Tian, Y., Xu, Z., Zheng, B., Martin Lo, Y., Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) seed oil (2013) Ultrason. Sonochem., 20, pp. 202-208Albu, S., Joyce, E., Paniwnyk, L., Lorimer, J.P., Mason, T.J., Potential for the use of ultrasound in the extraction of antioxidants from Rosmarinus officinalis for the food and pharmaceutical industry (2004) Ultrason. Sonochem., 11, pp. 261-265Lianfu, Z., Zelong, L., Optimization and comparison of ultrasound/microwave assisted extraction (UMAE) and ultrasonic assisted extraction (UAE) of lycopene from tomatoes (2008) Ultrason. Sonochem., 15, pp. 731-737Boonkird, S., Phisalaphong, C., Phisalaphong, M., Ultrasound-assisted extraction of capsaicinoids from Capsicum frutescens on a lab- and pilot-plant scale (2008) Ultrason. Sonochem., 15, pp. 1075-1079Barbero, G., Liazid, A., Palma, M., Barroso, C., Ultrasound-assisted extraction of capsaicinoids from peppers (2008) Talanta, 75, pp. 1332-1337Riera, E., Golás, Y., Blanco, A., Gallego, J.A., Blasco, M., Mulet, A., Mass transfer enhancement in supercritical fluids extraction by means of power ultrasound (2004) Ultrason. Sonochem., 11, pp. 241-244Balachandran, S., Kentish, S.E., Mawson, R., Ashokkumar, M., Ultrasonic enhancement of the supercritical extraction from ginger (2006) Ultrason. Sonochem., 13, pp. 471-479Hu, A.-J., Zhao, S., Liang, H., Qiu, T.-Q., Chen, G., Ultrasound assisted supercritical fluid extraction of oil and coixenolide from adlay seed (2007) Ultrason. Sonochem., 14, pp. 219-224(1998) Method of Determining and Expressing Fineness of Feed Materials by Sieving, pp. 447-550. , A.S.A.E. American Society of Agricultural Engineers StandardsByers, J.A., (2009) Catálogo PhenomenexDuarte, C., Moldão-Martins, M., Gouveia, A.F., Da Costa, S.B., Leitão, A.E., Bernardo-Gil, M.G., Supercritical fluid extraction of red pepper (Capsicum frutescens L.) (2004) J. Supercrit. Fluids, 30, pp. 155-161Daood, H.G., Illés, V., Gnayfeed, M.H., Mészáros, B., Horváth, G., Biacs, P.A., Extraction of pungent spice paprika by supercritical carbon dioxide and subcritical propane (2002) J. Supercrit. Fluids, 23, pp. 143-152Singleton, V.L., Orthofer, R., Lamuela-Raventós, R.M., Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent (1999) Meth. Enzymol., pp. 152-178. , P. Lester, Academic PressSovová, H., Rate of the vegetable oil extraction with supercritical CO2-I. Modelling of extraction curves (1994) Chem. Eng. Sci., 49, pp. 409-414Powell, M.J.D., (2009) Subroutine BOBQYA, , Department of Applied Mathematics and Theoretical Physics, Cambridge UniversitySilva, L.P.S., Mathematical Modeling of Mass Transfer in Supercritical Fluid Extraction Process from Red Pepper, , (Masters thesis), Food Engineering Postgraduation Programm of School of Food Engineering of the University of Campinas, Campinas, São Paulo, 2013Nogueira, R.I., Cornejo, F.E.P., Bizzo, H.R., Antoniassi, R., Freitas, S.P., Junior, W.F.L., Effects of drying parameters on pepper (Capsicum spp.) quality (2005) Fourth Mercosur Congress on Process Systems Engineering (ENPROMER), , Costa Verde, Rio de JaneiroJordão, P.R., Bonnas, D.S., Estudo das características físicas, físico- químicas e químicas de duas cultivares de pimenta, objetivando a industrialização (1996) Rev. Bras. Anal. Alim., pp. 36-37Shahidi, F., Naczk, M., (2003) Phenolics in Food and Nutraceuticals, , CRC Press Boca RatonEstrada, B., Pomar, F., Diíaz, J., Merino, F., Bernal, M.A., Pungency level in fruits of the Padrón pepper with different water supply (1999) Sci. Hortic., 81, pp. 385-396Estrada, B., Pomar, F., Díaz, J., Merino, F., Bernal, M.A., Effects of mineral fertilizer supplementation on fruit development and pungency in 'Padron' peppers (1998) J. Hortic. Sci. Biotechnol., 73, pp. 493-497Kwon, K.-T., Uddin, M.S., Jung, G.-W., Sim, J.-E., Lee, S.-M., Woo, H.-C., Chun, B.-S., Solubility of red pepper (Capsicum annum) oil in near- and supercritical carbon dioxide and quantification of capsaicin (2011) Korean J. Chem. Eng., 28, pp. 1433-1438Markom, M., Hasan, M., Daud, W.R.W., Singh, H., Jahim, J.M., Extraction of hydrolysable tannins from Phyllanthus niruri Linn.: Effects of solvents and extraction methods (2007) Sep. Purif. Technol., 52, pp. 487-496Mazzutti, S., Ferreira, S.R.S., Riehl, C.A.S., Smania, Jr.A., Smania, F.A., Martínez, J., Supercritical fluid extraction of Agaricus brasiliensis: Antioxidant and antimicrobial activities (2012) J. Supercrit. Fluids, 70, pp. 48-56Barbero, G.F., Palma, M., Barroso, C.G., Pressurized liquid extraction of capsaicinoids from peppers (2006) J. Agric. Food Chem., 54, pp. 3231-3236Del Valle, J.M., Jiménez, M., De La Fuente, J.C., Extraction kinetics of pre-pelletized Jalapeño peppers with supercritical CO2 (2003) J. Supercrit. Fluids, 25, pp. 33-44Biesaga, M., Influence of extraction methods on stability of flavonoids (2011) J. Chromatogr. A, 1218, pp. 2505-2512Reverchon, E., De Marco, I., Supercritical fluid extraction and fractionation of natural matter (2006) J. Supercrit. Fluids, 38, pp. 146-166Yu, J., Wang, J., Liu, C., Liu, Z., Wang, Q., Application of response surface methodology to optimise supercritical carbon dioxide extraction of oil from rapeseed (Brassica napus L.) (2012) Int. J. Food Sci. Technol., 47, pp. 1115-1121Weinhold, T.D.S., Bresciani, L.F.V., Tridapalli, C.W., Yunes, R.A., Hense, H., Ferreira, S.R.S., Polygala cyparissias oleoresin: Comparing CO2 and classical organic solvent extractions (2008) Chem. Eng. Process., 47, pp. 109-117Bird, B., Stewart, W., Lightfoot, E., (2006) Transport Phenomena, , second ed. John Wiley & Sons IncYing, Z., Han, X., Li, J., Ultrasound-assisted extraction of polysaccharides from mulberry leaves (2011) Food Chem., 127, pp. 1273-1279Chen, F., Sun, Y., Zhao, G., Liao, X., Hu, X., Wu, J., Wang, Z., Optimization of ultrasound-assisted extraction of anthocyanins in red raspberries and identification of anthocyanins in extract using high-performance liquid chromatography-mass spectrometry (2007) Ultrason. Sonochem., 14, pp. 767-778Chittapalo, T., Noomhorm, A., Ultrasonic assisted alkali extraction of protein from defatted rice bran and properties of the protein concentrates (2009) Int. J. Food Sci. Technol., 44, pp. 1843-1849Zhang, Z.-S., Wang, L.-J., Li, D., Jiao, S.-S., Chen, X.D., Mao, Z.-H., Ultrasound-assisted extraction of oil from flaxseed (2008) Sep. Purif. Technol., 62, pp. 192-198