| dc.creator | Aguiar V.S. | |
| dc.creator | Bottoli C.B.G. | |
| dc.date | 2015 | |
| dc.date | 2015-06-25T12:52:30Z | |
| dc.date | 2015-11-26T15:04:37Z | |
| dc.date | 2015-06-25T12:52:30Z | |
| dc.date | 2015-11-26T15:04:37Z | |
| dc.date.accessioned | 2018-03-28T22:15:26Z | |
| dc.date.available | 2018-03-28T22:15:26Z | |
| dc.identifier | | |
| dc.identifier | Instrumentation Science And Technology. Taylor And Francis Inc., v. 43, n. 2, p. 139 - 155, 2015. | |
| dc.identifier | 10739149 | |
| dc.identifier | 10.1080/10739149.2014.954126 | |
| dc.identifier | http://www.scopus.com/inward/record.url?eid=2-s2.0-84921517842&partnerID=40&md5=784327460f3ee8cca500249f834ed3c3 | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/85392 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/85392 | |
| dc.identifier | 2-s2.0-84921517842 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1256887 | |
| dc.description | Octadecyl methacrylate-based monolithic capillary columns were prepared using octadecyl methacrylate as the monomer, ethylene dimethacrylate as the cross-linking agent, 2-acryloylamido-2-methylpropanesulfonic acid as the ionizable monomer, responsible for a negatively charged stationary phase surface and able to support a cathodic electrosmotic flow, and amyl alcohol and 1,4-butanediol as porogenic solvents. The repeatability of the morphological aspect of the monolithic material was evaluated by scanning electron microscopy (SEM). The uniformity along the same monolithic bed was also investigated by SEM. Replicate determinations of surface area and pore volume were also performed and compared. The repeatability of the obtained electrochromatographic efficiencies for separations of alkylbenzenes using the stationary phases prepared was also evaluated. A column that had a plate height of 31 μm was tested for separation of polycyclic aromatic hydrocarbons. Even though minimal possible variation of experimental parameters was maintained, it was not possible to reproduce the stationary phases of the monolithic columns, since the relative standard deviation for the efficiencies obtained on columns prepared from the same polymerization mixture composition was 62%. The lack of repeatability for the efficiency is thought to be a result of limited homogeneity of the monolithic globular structures along the length of the majority of the prepared columns. The poor precision of preparation was justified by the copolymerization reaction that occurs in the presence of 2, 2′-azobisisobutyronitrile as the initiator agent, whose decomposition starts at the moment that this is added to the reaction mixture. | |
| dc.description | 43 | |
| dc.description | 2 | |
| dc.description | 139 | |
| dc.description | 155 | |
| dc.description | Tang, Q., Lee, M.L., Column Technology for Capillary Electrochromatography (2000) Trends Anal. Chem., 19, pp. 648-663 | |
| dc.description | Eeltink, S., Kok, W.Th., Recent Applications in Capillary Electrochromatography (2006) Electrophoresis, 27, pp. 84-96 | |
| dc.description | Eeltink, S., Svec, F., Fréchet, J.M.J., Open-tubular Capillary Columns with a Porous Layer of Monolithic Polymer for Highly Efficient and Fast Separations in Electrochromatography (2006) Electrophoresis, 27, pp. 4249-4256 | |
| dc.description | Li, W., Fries, D.P., Malik, A., Sol-Gel Stationary Phases for Electrochromatography (2004) J. Chromatogr. A, 1044, pp. 23-52 | |
| dc.description | Zajickova, Z., Luna, J., Svec, F., Surface Modification of Silica-based Monolith with Poly(pentafluoropropyl methacrylate) Using Single Step Photografting (2010) J. Liq. Chromatogr. Relat. Technol., 33, pp. 1640-1648 | |
| dc.description | Planeta, J., Moravcová, D., Roth, M., Karásek, P., Kahle, V., Silica-based Monolithic Capillary Columns - Effect of Preparation Temperature on Separation Efficiency (2010) J. Chromatogr. A, 1217, pp. 5737-5740 | |
| dc.description | Bernabé-Zafón, V., Cantó-Mirapeix, A., Simó-Alfonso, E.F., Ramis-Ramos, G., Herrero-Martínez, J.M., Comparison of Thermal and Photo Polymerization of Lauryl Methacrylate Monolithic Columns for CEC (2009) Electrophoresis, 30, pp. 1929-1936 | |
| dc.description | Kositarat, S., Smith, N.W., Nacapricha, D., Wilairat, P., Chaisuwan, P., Repeatability in Column Preparation of a Reversed-phase C18 Monolith and its Application to Separation of Tocopherol Homologues (2011) Talanta, 84, pp. 1374-1378 | |
| dc.description | Nischang, I., Teasdale, I., Brüggemann, O., Towards Porous Polymer Monoliths for the Efficient, Retention-independent Performance in the Isocratic Separation of Small Molecules by Means of Nano-liquid Chromatography (2010) J. Chromatogr. A, 1217, pp. 7514-7522 | |
| dc.description | Ludewig, R., Nietzsche, S., Scriba, G.K.E., A Weak Cation-exchange Monolith as Stationary Phase for the Separation of Peptide Diastereomers by CEC (2011) J. Sep. Sci., 34, pp. 64-69 | |
| dc.description | Yamada, H., Kitagawa, S., Ohtani, H., Simultaneous Separation of Water- and Fat-soluble Vitamins in Isocratic Pressure-assisted Capillary Electrochromatography Using a Methacrylate-based Monolithic Column (2013) J. Sep. Sci., 36, pp. 1980-1985 | |
| dc.description | Geiser, L., Eeltink, S., Svec, F., Fréchet, J.M.J., Stability and Repeatability of Capillary Columns Based on Porous Monoliths of Poly(butyl methacrylate-co-ethylene dimethacrylate) (2007) J. Chromatogr. A, 1140, pp. 140-146 | |
| dc.description | Nischang, I., Svec, F., Fréchet, J.M.J., Effect of Capillary Cross-section Geometry and Size on the Separation of Proteins in Gradient Mode Using Monolithic Poly(butyl methacrylate-co-ethylene dimethacrylate) Columns (2009) J. Chromatogr. A, 1216, pp. 2355-2361 | |
| dc.description | Deyl, Z., Svec, F., (2001) Capillary Electrochromatography, , Elsevier Science B.V.: Berkeley, CA | |
| dc.description | Smith, N.W., Jiang, Z., Developments in the Use and Fabrication of Organic Monolithic Phases for Use with High-performance Liquid Chromatography and Capillary Electrochromatography (2008) J. Chromatogr. A, 1184, pp. 416-440 | |
| dc.description | Svec, F., Recent Developments in the Field of Monolithic Stationary Phases for Capillary Electrochromatography (2005) J. Sep. Sci., 28, pp. 729-745 | |
| dc.description | Urban, J., Svec, F., Fréchet, J.M.J., Hypercrosslinking: New Approach to Porous Polymer Monolithic Capillary Columns of Large Surface Area for the Highly Efficient Separation of Small Molecules (2010) J. Chromatogr. A, 1217, pp. 8212-8221 | |
| dc.description | Lubbad, S.H., Buchmeiser, M.R., Fast Separation of Low Molecular Weight Analytes on Structurally Optimized Polymeric Capillary Monoliths (2010) J. Chromatogr. A, 1217, pp. 3223-3230 | |
| dc.description | Mohr, J.H., Swart, R., Huber, C.G., Morphology and Efficiency of Poly(styrene-co-divinylbenzene)-based Monolithic Capillary Columns for the Separation of Small and Large Molecules (2011) Anal. Bioanal. Chem., 400, pp. 2391-2402 | |
| dc.description | Umemura, T., Ueki, Y., Tsunoda, K., Katakai, A., Tamada, M., Haraguchi, H., Preparation and Characterization of Methacrylate-based Semi-micro Monoliths for High-throughput Bioanalysis (2006) Anal. Bioanal. Chem., 386, pp. 566-571 | |
| dc.description | Eeltink, S., Rozing, G.P., Schoenmakers, P.J., Kok, W.Th., Practical Aspects of Using Methacrylate-esterbased Monolithic Columns in Capillary Electrochromatography (2006) J. Chromatogr. A, 1109, pp. 74-79 | |
| dc.description | Eeltink, S., Geiser, L., Svec, F., Fréchet, J.M.J., Optimization of the Porous Structure and Polarity of Polymethacrylate-based Monolithic Capillary Columns for the LC-MS Separation of Enzymatic Digests (2007) J. Sep. Sci., 30, pp. 2814-2820 | |
| dc.description | Wieder, W., Lubbad, S.H., Trojer, L., Bisjak, C.P., Bonn, G.K., Novel Monolithic Poly(p-methacrylate-co-bis(p-vinylbenzyl)dimethylsilane) Capillary Columns for Biopolymer Separation (2008) J. Chromatogr. A, 1191, pp. 253-262 | |
| dc.description | Aguiar, V.S., Bottoli, C.B.G., Development and Characterization of Hydrophobic Organic Monolithic Columns for Use in Capillary Electrochromatography (2013) Microchem. J., 109, pp. 51-57 | |
| dc.description | Courtois, J., Szumski, M., Georgsson, F., Irgum, K., Assessing the Macroporous Structure of Monolithic Columns by Transmission Electron Microscopy (2007) Anal. Chem., 79, pp. 335-344 | |
| dc.description | Buszewski, B., Szumski, M., Study of Bed Homogeneity of Methacrylate-based Monolithic Columns for Micro-HPLC and CEC (2004) Chromatographia Supplement, 60, pp. S261-S267 | |
| dc.description | Svec, F., Peters, E.C., Sýkora, D., Fréchet, J.M.J., Design of the Monolithic Polymers Used in Capillary Electrochromatography Columns (2000) J. Chromatogr. A, 887, pp. 3-29 | |
| dc.description | Hilder, E.F., Svec, F., Fréchet, J.M.J., Polymeric Monolithic Stationary Phases for Capillary Electrochromatography (2002) Electrophoresis, 23, pp. 3934-3953 | |
| dc.description | Xu, G., Nambiar, R.R., Blum, F.D., Room-temperature Decomposition of 2,2′-azobis(isobutyronitrile) in Emulsion Gels with and without Silica (2006) J. Colloid Interface Sci., 302, pp. 658-661 | |
| dc.description | Nischang, I., Teasdale, I., Brüggemann, O., Porous Polymer Monoliths for Small Molecule Separations: Advancements and Limitations (2011) Anal. Bioanal. Chem., 400, pp. 2289-2304 | |
| dc.language | en | |
| dc.publisher | Taylor and Francis Inc. | |
| dc.relation | Instrumentation Science and Technology | |
| dc.rights | fechado | |
| dc.source | Scopus | |
| dc.title | Repeatability Of Octadecyl Methacrylate-based Monolithic Columns For Capillary Electrochromatography | |
| dc.type | Artículos de revistas | |
