The present study evaluated the use of polyethylene film wrapping of beef half carcasses and its effects on cooler shrink, cooling characteristics and microbial status of the half carcasses. Film wrapping reduced cooler shrink by 55.2%, 43.1%, 36.0% and 30% after 24, 48, 72 and 96. h of cooling, respectively, compared to the unwrapped half carcasses, whereas the surface water activity showed no significant differences among the time periods. The wrapped half carcasses had a lower cooling rate and higher surface and internal temperatures. The highest values of the aerobic mesophiles, Staphylococcus aureus and Enterobacteriaceae were found in the half carcasses wrapped in film. No significant differences were found in the values of Escherichia coli. The polyethylene film was effective in reducing cooler shrink; however, it caused a delay in cooling, thereby enabling greater microbial occurrences and counts and impairing the hygienic and sanitary conditions of the carcasses, which may be an impediment to the practical application of this technology.100 164170(2012), 19th ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, Official Method 990.12(2012), 19th ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, Official Method 2003.11(2012), 19th ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, Official Method 2003.01(2012), 19th ed., AOAC INTERNATIONAL, Gaithersburg, MD, USA, Official Method 998.08AQIS Notice Meat 2007/12 (2007) ESAM (E. coli-Salmonella) TVC sampling of carcasses in EU listed establishmentsAQIS Notice Meat 2003/06 (2003) Revised ESAM programArgudín, M.A., Mendoza, M.C., Rodicio, M.R., Food poisoning and Staphylococcus aureus enterotoxins (2010) Toxins, 2, pp. 1751-1773Brasil. Ministério da Agricultura Pecuária e Abastecimento. Portaria n° 5, de 8 de novembro de 1988 (1988) Aprova o novo Sistema Nacional de Tipificação de Carcaças Bovinas, , Brasil Diário Oficial [da] União, Brasília, DF, BrasilBrasil. Ministério da Agricultura Pecuária e Abastecimento. Portaria n° 612, de 5 de outubro de 1989 (1989) Aprova a Padronização dos Cortes de Carne Bovina, , Brasil Diário Oficial [da] União, Brasília, DF, BrasilBrasil. Ministério da Saúde. Agencia Nacional de Vigilância Sanitária (2001) RDC n° 12, de 2 de jan. de 2001. Aprova o regulamento técnico sobre padrões microbiológicos para alimentos, , Brasil Diário Oficial [da] União, Brasília, DF, BrasilMinistério da Agricultura, Pecuária e Abastecimento (2010) Regulation of industrial and sanitary inspection of animal products, , Brasil (Brasília, DF, Brasil)Buncic, S., Nychas, G., Lee, M.R.F., Koutsoumanis, K., Hébraud, M., Desvaux, M., Chorianopoulos, N., Antic, D., Microbial pathogen control in the beef chain: Recent research advances (2014) Meat Science, 97, pp. 375-383Crowley, K.M., Prendergast, D.M., Sheridan, J.J., McDowell, D.A., Survival of Pseudomonas fluorescens on beef carcass surface in a commercial abattoir (2010) Meat Science, 85, pp. 550-554Duffy, G., Burgess, C.M., Bolton, D.J., A review of factors that effect transmission and survival of verocytotoxigenic Escherichia coli in the European farm to fork beef chain (2014) Meat Science, 97, pp. 375-383European Food Safety Authority. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2011 (2013) EFSA Journal, 11 (4), p. 3129. , (250pp.)European Food Safety Authority. Scientific opinion on the public health hazards to be covered by inspection of meat (bovine animals) (2013) EFSA Journal, 11 (6), p. 3266. , (261pp.)Commission Regulation (EC) 1441 of 05 Dez. 2007. Amending Regulation (EC) No 2073/2005 on microbiological criteria for foodstuffs (2007) Official Journal of the European Union, 1322, pp. 12-29Fernández, A.M., Vieira, C., Effect of chilling applied to suckling lamb carcasses on hygienic, physicochemical and sensory meat quality (2012) Meat Science, 92, pp. 569-574Ferraz, J.B.S., Felicio, P.E., Production systems - An example from Brazil (2010) Meat Science, 84 (2), pp. 238-243Fisher, A.V., Bayntun, J.A., Weight loss from beef sides during storage post mortem and its effects on carcass composition (1983) Meat Science, 9 (2), pp. 121-129Gill, C.O., Bryant, J., Landers, C., Identification of critical control points for control of microbiological contamination in processes leading to the production of ground beef at a packing plant (2003) Food Microbiology, 20 (6), pp. 641-650Gill, C.O., Landers, C., Effects of spray-cooling processes on the microbiological conditions of decontaminated beef carcasses (2003) Journal of Food Protection, 66 (7), pp. 1247-1252Goodman, L.A., Simultaneous confidence interval for contrasts among multinomial populations (1964) Annals of Mathematical Statistics, 35 (2), pp. 716-725Goodman, L.A., On simultaneous confidence intervals for multinomial proportions (1965) Technometrics, 7 (2), pp. 247-254Greig, J.D., Waddell, L., Wilhelm, B., Wilkins, W., Bucher, O., Parker, S., Rajić, A., The efficacy of interventions applied during primary processing on contamination of beef carcasses with Escherichia coli: A systematic review-meta-analysis of the published research (2012) Food Control, 27 (2), pp. 385-397Huber, H., Ziegler, D., Pflüger, V., Vogel, G., Zweifel, C., Stephan, R., Prevalence and characteristics of methicillin-resistant coagulase-negative staphylococci from livestock, chicken carcasses, bulk tank milk, minced meat, and contact persons (2011) BMC Veterinary Research, 7 (1), p. 6International Organization for Standardization. ISO 17604:2003 (2003) Microbiology of food and animal feeding stuffs - Carcass sampling for microbiological analysisKinsella, K.J., Sheridan, J.J., Rowe, T.A., Butler, F., Delgado, A., Quispe-Ramirez, A., Blair, I.S., McDowell, D.A., Impact of a novel spray-chilling system on surface microflora, water activity and weight loss during beef carcass chilling (2006) Food Microbiology, 23, pp. 483-490Lage, J.F., Paulino, P.V.R., Pereira, L.G.R., Duarte, M.S., Oliveira, A.S., Souza, N.K.P., Lima, J.C.M., Carcass characteristics of feedlot lambs fed crude glycerin contaminated with high concentrations of crude fat (2014) Meat Science, 96 (1), pp. 108-113Lage, J.F., Paulino, P.V.R., Souza, E.J.O., Duarte, M.S., Benedeti, P.D.B., Souza, N.K.P., Cox, R.B., Influence of genetic type and level of concentrate in the finishing diet on carcass and meat quality traits in beef heifers (2012) Meat Science, 90 (3), pp. 770-774Lawrie, R.A., Ledward, D.A., (2006) Lawrie's meat science, , Woodhead Publishing Limited & New York: CRC Press LLC, CambridgeLazarus, C.R., West, R.L., Oblinger, J.L., Palmer, A.Z., Evaluation of a calcium alginate coating and a protective plastic wrapping for the control of lamb carcass shrinkage (1976) Journal of Food Science, 41 (3), pp. 639-641Miguel, G.Z., Faria, M.H., Roça, R.O., Santos, C.T., Suman, S.P., Faitarone, A.B.G., Delbem, N.L.C., Savian, T.V., Immunocastration improves carcass traits and beef color attributes in Nellore and Nellore X Aberdeen Angus crossbred animals finished in feedlot (2014) Meat Science, 96, pp. 884-891Milios, K.T., Drosinos, E.H., Zoiopoulos, P.E., Food Safety Management System validation and verification in meat industry: Carcass sampling methods for microbiological hygiene criteria - A review (2014) Food Control, 43, pp. 74-81Prado, C.S., Felicio, P.E., Effects of chilling rate and spray-chilling on weight loss and tenderness in beef strip loin steaks (2010) Meat Science, 86, pp. 430-435Rea, R.H., Smith, G.C., Carpenter, Z.L., Hoke, K.E., Protective packaging materials for fresh beef shipments (1972) Journal of Food Science, 37 (5), pp. 739-742Savell, J.W., Mueller, S.L., Baird, B.E., The chilling of carcasses (2005) Meat Science, 70, pp. 449-459Smith, G.C., Carpenter, Z.L., Post mortem shrinkage of lamb carcasses (1973) Journal of Animal Science, 36 (5), pp. 862-867Smith, G.C., Varnadore, W.L., Carpenter, Z.L., Calhoun, M.C., Postmortem treatment effects on lamb shrinkage, bacterial counts and palatability (1976) Journal of Animal Science, 42 (5), pp. 1167-1174Sofos, J.N., Kochevar, S.L., Reagan, J.O., Smith, G.C., Extent of beef carcass contamination with Escherichia coli and probabilities of passing US regulatory criteria (1999) Journal of Food Protection, 62 (3), pp. 234-238Tittor, A.W., Tittor, M.G., Brashears, M.M., Brooks, J.C., Garmyn, A.J., Miller, M.F., Effects of simulated dry and wet chilling and aging of beef fat and lean tissues on the reduction of Escherichia coli O157: H7 and Salmonella (2011) Journal of Food Protection, 74 (2), pp. 289-293United States Department of Agriculture (USDA). Foreign Agricultural Service. Office of Global Analysis (2014) Livestock and poultry: world markets and trade, , AprilUnited States of America. United States Department of Agriculture (USDA). Food Safety and Inspection Service. Pathogen ReductionHazard Analysis and Critical Control Point (HACCP) systemsfinal rule (1996) Federal register, 61, pp. 38805-38989Wiklund, E., Kemp, R.M., leRoux, G.J., Li, Y., Wu, G., Spray chilling of deer carcasses - Effects on carcass weight, meat moisture content, purge and microbiological quality (2010) Meat Science, 86 (4), pp. 926-930Zar, J.H., (2009) Biostatistical analysis, , Prentice-Hall, New JerseyZhou, G.H., Xu, X.L., Liu, Y., Preservation technologies for fresh meat - A review (2010) Meat Science, 86 (1), pp. 119-128Zweifel, C., Capek, M., Stephan, R., Microbiological contamination of cattle carcasses at different stages of slaughter in two abattoirs (2014) Meat Science, 98, pp. 198-202