Optical Tweezers And Multiphoton Microscopies Integrated Photonic Tool For Mechanical And Biochemical Cell Processes Studies

dc.creatorDe Thomaz A.A.
dc.creatorFaustino W.M.
dc.creatorFontes A.
dc.creatorFernandes H.P.
dc.creatorBarjas-Castro M.D.L.
dc.creatorMetze K.
dc.creatorGiorgio S.
dc.creatorBarbosa L.C.
dc.creatorCesar C.L.
dc.date2007
dc.date2015-06-30T18:37:40Z
dc.date2015-11-26T15:01:18Z
dc.date2015-06-30T18:37:40Z
dc.date2015-11-26T15:01:18Z
dc.date.accessioned2018-03-28T22:12:22Z
dc.date.available2018-03-28T22:12:22Z
dc.identifier9780819467928
dc.identifierProceedings Of Spie - The International Society For Optical Engineering. , v. 6644, n. , p. - , 2007.
dc.identifier0277786X
dc.identifier10.1117/12.734495
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-42149176524&partnerID=40&md5=bc24c427f91e739d9cb8ee998dba34d9
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/104060
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/104060
dc.identifier2-s2.0-42149176524
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1256268
dc.descriptionThe research in biomedical photonics is clearly evolving in the direction of the understanding of biological processes at the cell level. The spatial resolution to accomplish this task practically requires photonics tools. However, an integration of different photonic tools and a multimodal and functional approach will be necessary to access the mechanical and biochemical cell processes. This way we can observe mechanicaly triggered biochemical events or biochemicaly triggered mechanical events, or even observe simultaneously mechanical and biochemical events triggered by other means, e.g. electricaly. One great advantage of the photonic tools is its easiness for integration. Therefore, we developed such integrated tool by incorporating single and double Optical Tweezers with Confocal Single and Multiphoton Microscopies. This system can perform 2-photon excited fluorescence and Second Harmonic Generation microscopies together with optical manipulations. It also can acquire Fluorescence and SHG spectra of specific spots. Force, elasticity and viscosity measurements of stretched membranes can be followed by real time confocal microscopies. Also opticaly trapped living protozoas, such as leishmania amazonensis. Integration with CARS microscopy is under way. We will show several examples of the use of such integrated instrument and its potential to observe mechanical and biochemical processes at cell level.
dc.description6644
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dc.description
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dc.languageen
dc.publisher
dc.relationProceedings of SPIE - The International Society for Optical Engineering
dc.rightsaberto
dc.sourceScopus
dc.titleOptical Tweezers And Multiphoton Microscopies Integrated Photonic Tool For Mechanical And Biochemical Cell Processes Studies
dc.typeActas de congresos


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