Wet rot due to Pectobacterium carotovorum subsp. carotovorum is one of the main bacteria diseases that affect all potato cultivars causing significant losses. Potato plants contain glycoalkaloids being α-chaconine and α-solanine the main. The accumulation of these glycoalkaloids can be stimulated by several factors, especially light, having them important antimicrobial properties. The aim of this research was to evaluate how postharvest exposition to ultraviolet C (UV-C) and fluorescent light affects the development of P. carotovorum soft rot as well as the accumulation of α-chaconine and α-solanine, sprouting, weight loss and soluble solids content in potato seed tubers 'Agata' and 'Monalisa'. Susceptibility of P. carotovorum to UV-C light was first in vitro tested. For that, bacterial aliquots (107CFUmL-1) were grown in Petri dishes (culture medium YDC) and subjected to 0.0, 2.3, 6.9, 11.5 or 34.5kJm-2 of UV-C (254nm) and stored at 25°C in darkness. Number of colonies was counted after 24h. For in vivo analysis, potato seed tubers were subjected to UV-C (34.5kJm-2) with subsequent storage of half of the samples in darkness and the other half under fluorescent light (photon flux of 1.6μmolm-2s-1) at 25°C and 88% RH during 21 days. Development (incidence) and severity of wet rot, concentration of α-chaconine and α-solanine, sprouting, and quality parameters were analyzed. Non UV-C treated tubers were used as control. UV-C light at 34.5kJm-2 completely inhibited the development of P. carotovorum subsp. carotovorum in in vitro studies. For in vivo experiments, the control and the UV-C treated tubers stored under fluorescent light were less affected by soft rot than the UV-C treated stored under darkness since any disease incidence was detected on them. 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