Neutralisation Of The Pharmacological Activities Of Bothrops Alternatus Venom By Anti-pla2 Iggs

dc.creatorGarcia Denegri M.E.
dc.creatorMarunak S.
dc.creatorTodaro J.S.
dc.creatorPonce-Soto L.A.
dc.creatorAcosta O.
dc.creatorLeiva L.
dc.date2014
dc.date2015-06-25T18:01:07Z
dc.date2015-11-26T14:56:42Z
dc.date2015-06-25T18:01:07Z
dc.date2015-11-26T14:56:42Z
dc.date.accessioned2018-03-28T22:08:39Z
dc.date.available2018-03-28T22:08:39Z
dc.identifier
dc.identifierToxicon. Elsevier Ltd, v. 86, n. , p. 89 - 95, 2014.
dc.identifier410101
dc.identifier10.1016/j.toxicon.2014.05.016
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84902210184&partnerID=40&md5=de8711aa8c028d2d2d2e68533de2b7e2
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/87500
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/87500
dc.identifier2-s2.0-84902210184
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1255511
dc.descriptionBasic phospholipases A2 (PLA2) are toxic and induce a wide spectrum of pharmacological effects, although the acidic enzyme types are not lethal or cause low lethality. Therefore, it is challenging to elucidate the mechanism of action of acidic phospholipases. This study used the acidic non-toxic Ba SpII RP4 PLA2 from Bothrops alternatus as an antigen to develop anti-PLA2 IgG antibodies in rabbits and used in vivo assays to examine the changes in crude venom when pre-incubated with these antibodies. Using Ouchterlony and western blot analyses on B. alternatus venom, we examined the specificity and sensitivity of phospholipase A2 recognition by the specific antibodies (anti-PLA2 IgG). Neutralisation assays using a non-toxic PLA2 antigen revealed unexpected results. The (indirect) haemolytic activity of whole venom was completely inhibited, and all catalytically active phospholipases A2 were blocked. Myotoxicity and lethality were reduced when the crude venom was pre-incubated with anti-PLA 2 immunoglobulins. CK levels in the skeletal muscle were significantly reduced at 6 h, and the muscular damage was more significant at this time-point compared to 3 and 12 h. When four times the LD50 was used (224 μg), half the animals treated with the venom-anti PLA2 IgG mixture survived after 48 h. All assays performed with the specific antibodies revealed that Ba SpII RP4 PLA2 had a synergistic effect on whole-venom toxicity. IgG antibodies against the venom of the Argentinean species B. alternatus represent a valuable tool for elucidation of the roles of acidic PLA2 that appear to have purely digestive roles and for further studies on immunotherapy and snake envenoming in affected areas in Argentina and Brazil. © 2014 Elsevier Ltd. All rights reserved.
dc.description86
dc.description
dc.description89
dc.description95
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dc.languageen
dc.publisherElsevier Ltd
dc.relationToxicon
dc.rightsfechado
dc.sourceScopus
dc.titleNeutralisation Of The Pharmacological Activities Of Bothrops Alternatus Venom By Anti-pla2 Iggs
dc.typeArtículos de revistas


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