Artículos de revistas
A Model System To Study The Lignification Process In Eucalyptus Globulus
Registro en:
Physiologia Plantarum. Blackwell Publishing Ltd, v. 152, n. 1, p. 17 - 31, 2014.
319317
10.1111/ppl.12152
2-s2.0-84906078930
Autor
Araujo P.
Cesarino I.
Mayer J.L.S.
Ferrari I.F.
Kiyota E.
Sawaya A.C.H.F.
Leme A.F.P.
Mazzafera P.
Institución
Resumen
Recalcitrance of plant biomass is closely related to the presence of the phenolic heteropolymer lignin in secondary cell walls, which has a negative effect on forage digestibility, biomass-to-biofuels conversion and chemical pulping. The genus Eucalyptus is the main source of wood for pulp and paper industry. However, when compared to model plants such as Arabidopsis thaliana and poplar, relatively little is known about lignin biosynthesis in Eucalyptus and only a few genes were functionally characterized. An efficient, fast and inexpensive in vitro system was developed to study lignification in Eucalyptus globulus and to evaluate the potential role of candidate genes in this biological process. Seedlings were grown in four different conditions, in the presence or absence of light and with or without sucrose in the growth medium, and several aspects of lignin metabolism were evaluated. Our results showed that light and, to a lesser extent, sucrose induced lignin biosynthesis, which was followed by changes in S/G ratio, lignin oligomers accumulation and gene expression. In addition, higher total peroxidase activity and differential isoperoxidase profile were observed when seedlings were grown in the presence of light and sucrose. Peptide sequencing allowed the identification of differentially expressed peroxidases, which can be considered potential candidate class III peroxidases involved in lignin polymerization in E. globulus. © 2014 Scandinavian Plant Physiology Society. 152 1 17 31 Barcelo, A.R., Aznar-Asensio, G.J., Coniferyl alcohol oxidase activity of a cell-wall-located class III peroxidase (1999) Aust J Plant Physiol, 26, pp. 411-419 Barcelo, A.R., Munoz, R., Sabater, F., Lupin peroxidases. 1. Isolation and characterization of cell wall-bound isoperoxidase activity (1987) Physiol Plant, 71, pp. 448-454 Boerjan, W., Ralph, J., Baucher, M., Lignin biosynthesis (2003) Annu Rev Plant Biol, 54, pp. 519-546 Bonawitz, N., Chapple, C., The genetics of lignin biosynthesis: connecting genotype to phenotype (2010) Annu Rev Genet, 44, pp. 337-363 Cesarino, I., Araújo, P., Domingues, A., An overview of lignin metabolism and its effect on biomass recalcitrance (2012) Braz J Bot, 35, pp. 303-311 Cesarino, I., Araújo, P., Sampaio Mayer, J., Paes Leme, A., Mazzafera, P., Enzymatic activity and proteomic profile of class III peroxidases during sugarcane stem development (2012) Plant Physiol Biochem, 55, pp. 66-76 Cesarino, I., Araújo, P., Paes Leme, A., Creste, S., Mazzafera, P., Suspension cell culture as a tool for the characterization of class III peroxidases in sugarcane (2013) Plant Physiol Biochem, 62, pp. 1-10 Cetinkol, O., Smith-Moritz, A., Cheng, G., Lao, J., George, A., Hong, K., Henry, R., Holmes, B., Structural and chemical characterization of hardwood from tree species with applications as bioenergy feedstocks (2012) PLoS One, 7, pp. e52820 Chen, F., Dixon, R., Lignin modification improves fermentable sugar yields for biofuel production (2007) Nat Biotechnol, 25, pp. 759-761 Chen, Y.-A., Shin, J.-W., Liu, Z.-H., Effect of light on peroxidase and lignin synthesis in mungbean hypocotyls (2002) Plant Physiol Biochem, 40, pp. 33-39 Cheng, H., Zhang, Q., Guo, D., Genes that respond to H2O2 are also evoked under light in Arabidopsis (2012) Mol Plant, 6, pp. 226-228 Cosio, C., Dunand, C., Specific functions of individual class III peroxidase genes (2009) J Exp Bot, 60, pp. 391-408 Cosio, C., Dunand, C., Transcriptome analysis of various flower and silique development stages indicates a set of class III peroxidase genes potentially involved in pod shattering in Arabidopsis thaliana (2010) BMC Genomics, 11, p. 528 Cosio, C., Vuillemin, L., De Meyer, M., Kevers, C., Penel, C., Dunand, C., An anionic class III peroxidase from zucchini may regulate hypocotyl elongation through its auxin oxidase activity (2009) Planta, 229, pp. 823-836 Demura, T., Fukuda, H., Transcriptional regulation in wood formation (2007) Trends Plant Sci, 12, pp. 64-70 Ferri, M., Righetti, L., Tassoni, A., Increasing sucrose concentrations promote phenylpropanoid biosynthesis in grapevine cell cultures (2011) J Plant Physiol, 168, pp. 189-195 Foucart, C., Paux, E., Ladouce, N., San-Clemente, H., Grima-Pettenati, J., Sivadon, P., Transcript profiling of a xylem vs phloem cDNA subtractive library identifies new genes expressed during xylogenesis in Eucalyptus (2006) New Phytol, 170, pp. 739-752 Friedrich, J.B., Peter, J.D., Indole-3-acetic acid levels after phytochrome-mediated changes in the stem elongation rate of dark- and light-grown Pisum seedlings (1992) Planta, 188, pp. 85-92 Gion, J.-M., Carouché, A., Deweer, S., Bedon, F., Pichavant, F., Charpentier, J.-P., Baillères, H., Plomion, C., Comprehensive genetic dissection of wood properties in a widely-grown tropical tree: Eucalyptus (2011) BMC Genomics, 12, p. 301 Goicoechea, M., Lacombe, E., Legay, S., Mihaljevic, S., Rech, P., Jauneau, A., Lapierre, C., Grima-Pettenati, J., EgMYB2, a new transcriptional activator from Eucalyptus xylem, regulates secondary cell wall formation and lignin biosynthesis (2005) Plant J, 43, pp. 553-567 Gómez Ros, L., Gabaldón, C., Pomar, F., Merino, F., Pedreño, M., Barceló, A., Structural motifs of syringyl peroxidases predate not only the gymnosperm-angiosperm divergence but also the radiation of tracheophytes (2007) New Phytol, 173, pp. 63-78 Grabber, J.H., Schatz, P.F., Kim, H., Lu, F.C., Ralph, J., Identifying new lignin bioengineering targets: 1. Monolignol-substitute impacts on lignin formation and cell wall fermentability (2010) BMC Plant Biol, 10, p. 114 Guo, D., Chen, F., Wheeler, J., Winder, J., Selman, S., Peterson, M., Dixon, R., Improvement of in-rumen digestibility of alfalfa forage by genetic manipulation of lignin O-methyltransferases (2001) Transgenic Res, 10, pp. 457-464 Hall, T., BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Res, 41, pp. 95-98 Harkin, J., Obst, J., Lignification in trees: indication of exclusive peroxidase participation (1973) Science, 180, pp. 296-298 Hatfield, R., Fukushima, R.S., Can lignin be accurately measured? (2005) Crop Sci, 45, pp. 832-839 Hemm, M., Rider, S., Ogas, J., Murry, D., Chapple, C., Light induces phenylpropanoid metabolism in Arabidopsis roots (2004) Plant J, 38, pp. 765-778 Hiraga, S., Sasaki, K., Ito, H., Ohashi, Y., Matsui, H., A large family of class III plant peroxidases (2001) Plant Cell Physiol, 42, pp. 462-468 Hirakawa, H., Nakamura, Y., Kaneko, T., Isobe, S., Sakai, H., Kato, T., Hibino, T., Sato, S., Survey of the genetic information carried in the genome of Eucalyptus camaldulensis (2011) Plant Biotechnol, 28, pp. 471-480 Huntley, S., Ellis, D., Gilbert, M., Chapple, C., Mansfield, S., Significant increases in pulping efficiency in C4H-F5H-transformed poplars: improved chemical savings and reduced environmental toxins (2003) J Agric Food Chem, 51, pp. 6178-6183 Kärkönen, A., Koutaniemi, S., Lignin biosynthesis studies in plant tissue cultures (2010) J Integr Plant Biol, 52, pp. 176-185 Kiyota, E., Mazzafera, P., Sawaya, A.C.H.F., Analysis of soluble lignin in sugarcane by ultrahigh performance liquid chromatography-tandem mass spectrometry with a Do-It-Yourself oligomer database (2012) Anal Chem, 84, pp. 7015-7020 Kubo, M., Udagawa, M., Nishikubo, N., Horiguchi, G., Yamaguchi, M., Ito, J., Mimura, T., Demura, T., Transcription switches for protoxylem and metaxylem vessel formation (2005) Genes Dev, 19, pp. 1855-1860 Laemmli, U., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685 Lapierre, C., Pollet, B., Monties, B., Rolando, C., Thioacidolysis of spruce lignin: GC-MS analysis of the main dimers recovered after Raney nickel desulphuration (1991) Holzforschung, 45, pp. 61-68 Legay, S., Sivadon, P., Blervacq, A.-S., Pavy, N., Baghdady, A., Tremblay, L., Levasseur, C., Grima-Pettenati, J., EgMYB1, an R2R3 MYB transcription factor from Eucalyptus negatively regulates secondary cell wall formation in Arabidopsis and poplar (2010) New Phytol, 188, pp. 774-786 Li, X., Weng, J.-K., Chapple, C., Improvement of biomass through lignin modification (2008) Plant J, 54, pp. 569-581 Lucas, W., Groover, A., Lichtenberger, R., Furuta, K., Yadav, S.-R., Helariutta, Y., He, X.-Q., Kachroo, P., The plant vascular system: evolution, development and functions (2013) J Integr Plant Biol, 55, pp. 294-388 Mika, A., Lüthje, S., Properties of guaiacol peroxidase activities isolated from corn root plasma membranes (2003) Plant Physiol, 132, pp. 1489-1498 Mitsuda, N., Iwase, A., Yamamoto, H., Yoshida, M., Seki, M., Shinozaki, K., Ohme-Takagi, M., NAC transcription factors, NST1 and NST3, are key regulators of the formation of secondary walls in woody tissues of Arabidopsis (2007) Plant Cell, 19, pp. 270-280 Morreel, K., Ralph, J., Kim, H., Lu, F.C., Goeminne, G., Ralph, S., Messens, E., Boerjan, W., Profiling of oligolignols reveals monolignol coupling conditions in lignifying poplar xylem (2004) Plant Physiol, 136, pp. 3537-3549 Morreel, K., Kim, H., Lu, F., Dima, O., Akiyama, T., Vanholme, R., Niculaes, C., Boerjan, W., Mass spectrometry-based fragmentation as an identification tool in lignomics (2010) Anal Chem, 82, pp. 8095-8105 Moura, J.C., Bonine, C.A., de Oliveira Fernandes Viana, J., Dornelas, M.C., Mazzafera, P., Abiotic and biotic stresses and changes in the lignin content and composition in plants (2010) J Integr Plant Biol, 52, pp. 360-376 Moura, J., Araújo, P., Brito Mdos, S., Souza, U.R., Viana Jde, O., Mazzafera, P., Validation of reference genes from Eucalyptus spp. under different stress conditions (2012) BMC Res Notes, 14, p. 634 Murashige, T., Skoog, F., A revised medium for rapid growth and bio assays with tobacco tissue cultures (1962) Physiol Plant, 15, pp. 473-497 Nemhauser, J., Chory, J., (2002) Arabidopsis Book, 10, pp. e0147. , Photomorphogenesis O'Brien, T., Feder, N., McCully, M., Polychromatic staining of plant cell walls by toluidine blue O (1964) Protoplasma, 59, pp. 368-373 Passardi, F., Cosio, C., Penel, C., Dunand, C., Peroxidases have more functions than a Swiss army knife (2005) Plant Cell Rep, 24, pp. 255-265 Passardi, F., Theiler, G., Zamocky, M., Cosio, C., Rouhier, N., Teixera, F., Margis-Pinheiro, M., Dunand, C., PeroxiBase: the peroxidase database (2007) Phytochemistry, 68, pp. 1605-1611 Raes, J., Rohde, A., Christensen, J., Van de Peer, Y., Boerjan, W., Genome-wide characterization of the lignification toolbox in Arabidopsis (2003) Plant Physiol, 133, pp. 1051-1071 Rencoret, J., Gutierrez, A., Nieto, L., Jimenez-Barbero, J., Faulds, C.B., Kim, H., Ralph, J., Del Rio, J.C., Lignin composition and structure in young versus adult Eucalyptus globulus plants (2011) Plant Physiol, 155, pp. 667-682 Rodrigues, J., Meier, D., Faix, O., Pereira, H., Determination of tree to tree variation in syringyl/guaiacyl ratio of Eucalyptus globulus wood lignin by analytical pyrolysis (1999) J Anal Appl Pyrol, 48, pp. 121-128 Rogers, L., Dubos, C., Cullis, I., Surman, C., Poole, M., Willment, J., Mansfield, S., Campbell, M., Light, the circadian clock, and sugar perception in the control of lignin biosynthesis (2005) J Exp Bot, 56, pp. 1651-1663 Ruegger, M., Meyer, K., Cusumano, J.C., Chapple, C., Regulation of ferulate-5-hydroxylase expression in Arabidopsis in the context of sinapate ester biosynthesis (1999) Plant Physiol, 119, pp. 101-110 Solfanelli, C., Poggi, A., Loreti, E., Alpi, A., Perata, P., Sucrose-specific induction of the anthocyanin biosynthetic pathway in Arabidopsis (2006) Plant Physiol, 140, pp. 637-646 Su, G., An, Z., Zhang, W., Liu, Y., Light promotes the synthesis of lignin through the production of H2O2 mediated by diamine oxidases in soybean hypocotyls (2005) J Plant Physiol, 162, pp. 1297-1303 Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S., MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods (2011) Mol Biol Evol, 28, pp. 2731-2739 Thomas, D.S., Traianos, A.Y., Athanasios, S.E., Expression of peroxidases during seedling growth in Ebenus cretica L. as affected by light and temperature treatments (2005) Plant Growth Regul, 46, pp. 143-151 Tokunaga, N., Kaneta, T., Sato, S., Sato, Y., Analysis of expression profiles of three peroxidase genes associated with lignification in Arabidopsis thaliana (2009) Physiol Plant, 136, pp. 237-249 Van Acker, R., Vanholme, R., Storme, V., Mortimer, J.C., Dupree, P., Boerjan, W., Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana (2013) Biotechnol Biofuels, 6, p. 46 Vanholme, R., Morreel, K., Ralph, J., Boerjan, W., Lignin engineering (2008) Curr Opin Plant Biol, 11, pp. 278-285 Wang, Y., Chantreau, M., Sibout, R., Hawkins, S., Plant cell wall lignification and monolignol metabolism (2013) Front Plant Sci, 4, p. 220 Weng, J.K., Chapple, C., The origin and evolution of lignin biosynthesis (2010) New Phytol, 187, pp. 273-285 Zhao, Q., Wang, H., Yin, Y., Xu, Y., Chen, F., Dixon, R., Syringyl lignin biosynthesis is directly regulated by a secondary cell wall master switch (2010) Proc Natl Acad Sci USA, 107, pp. 14496-14501 Zhong, R., Demura, T., Ye, Z.-H., SND1, a NAC domain transcription factor, is a key regulator of secondary wall synthesis in fibers of Arabidopsis (2006) Plant Cell, 18, pp. 3158-3170 Zhong, R., Lee, C., Ye, Z.-H., Functional characterization of poplar wood-associated NAC domain transcription factors (2010) Plant Physiol, 152, pp. 1044-1055 Ziebell, A., Gracom, K., Katahira, R., Chen, F., Pu, Y.Q., Ragauskas, A., Dixon, R.A., Davis, M., Increase in 4-coumaryl alcohol units during lignification in alfalfa (Medicago sativa) alters the extractability and molecular weight of lignin (2010) J Biol Chem, 285, pp. 38961-38968