In this work we evaluated ethanol production from enzymatic hydrolysis of sugarcane bagasse. Two pretreatments agents, lime and alkaline hydrogen peroxide, were compared in their performance to improve the susceptibility of bagasse to enzymatic action. Mild conditions of temperature, pressure and absence of acids were chosen to diminish costs and to avoid sugars degradation and consequent inhibitors formation. The bagasse was used as it comes from the sugar/ethanol industries, without grinding or sieving, and hydrolysis was performed with low enzymes loading (3.50 FPU g -1 dry pretreated biomass of cellulase and 1.00 CBU g -1 dry pretreated biomass of β-glucosidase). The pretreatment with alkaline hydrogen peroxide led to the higher glucose yield: 691 mg g -1 of glucose for pretreated bagasse after hydrolysis of bagasse pretreated for 1 h at 25 °C with 7.35% (v/v) of peroxide. Fermentation of the hydrolyzates from the two pretreatments were carried out and compared with fermentation of a glucose solution. Ethanol yields from the hydrolyzates were similar to that obtained by fermentation of the glucose solution. Although the preliminary results obtained in this work are promising for both pretreatments considered, reflecting their potential for application, further studies, considering higher biomass concentrations and economic aspects should be performed before extending the conclusions to an industrial process. © 2011 Elsevier Ltd.35726002607Baudel, H.M., Zaror, C., Abreu, C.A.M., Improving the value of sugarcane bagasse via integrated chemical production systems: an environmentally friendly approach (2005) Ind Crops Prod, 21, pp. 309-315Laser, M., Schulman, D., Allen, S.G., Lichwa, J., Antal, M.J., Lynd, L.R., A comparison of liquid hot water and steam pretreatments of sugar cane bagasse for bioconversion to ethanol (2002) Bioresour Technol, 81, pp. 33-44Lee, J., Biological conversion of lignocellulosic biomass to ethanol (1997) J Biotechnol, 56, pp. 1-24Mosier, N., Wyman, C., Dale, B., Elander, R., Lee, Y.Y., Holtzapple, M., Features of promising technologies for pretreatment of lignocellulosic biomass (2005) Bioresour Technol, 96, pp. 673-686Wyman, C.E., Dale, B.E., Elander, R.T., Holtzapple, M., Ladisch, M.R., Lee, Y.Y., Coordinated development of leading biomass pretreatment technologies (2005) Bioresour Technol, 96, pp. 1959-1966Wyman, C.E., Dale, B.E., Elander, R.T., Holtzapple, M., Ladisch, M.R., Lee, Y.Y., Comparative sugar recovery data from laboratory scale application of leading pretreatment technologies to corn stover (2005) Bioresour Technol, 96, pp. 2026-2032Amjed, M., Jung, H.G., Donker, J.D., Effect of alkaline hydrogen peroxide treatment on cell wall composition and digestion kinetics of sugarcane residues and wheat straw (1992) J Anim Sci, 70, pp. 2877-2884Saha, B.C., Loren, B.I., Cotta, M.A., Wu, Y.V., Dilute acid pretreatment, enzymatic saccharification and fermentation of wheat straw to ethanol (2005) Process Biochem, 40, pp. 3693-3700Teixeira, L.C., Linden, J.C., Herbert, H.A., Optimizing peracetic acid treatment conditions for improved simultaneous saccharification and co-fermentation of sugar cane bagasse to ethanol fuel (1999) Renew Energ, 16, pp. 1070-1073Martín, C., Galbe, M., Wahlbom, C.F., Hahn-Hägerdal, B., Jönsson, L.J., Ethanol production from enzymatic hydrolyzates of sugarcane bagasse using recombinant xylose-utilising S. cerevisiae (2002) Enzym Microb Technol, 31, pp. 274-282Kaar, W.E., Holtzapple, M.T., Using lime pretreatment to facilitate the enzymatic hydrolysis of corn stover (2000) Biomass Bioenergy, 18, pp. 189-199Azzam, A.M., Pretreatment of cane bagasse with hydrogen peroxide for enzymatic hydrolysis of cellulose and ethanol fermertation (1989) J Environ Sci Health B, 24, pp. 421-433Gould, J.M., Freer, S.N., High-efficiency ethanol production from lignocellulosic residues pretreated with alkaline H2O2 (1984) Biotechnol Bioeng, 26, pp. 628-631Gould, J.M., Studies on the mechanism of alkaline peroxide delignification of agricultural residues (1985) Biotechnol Bioeng, 27, pp. 225-231Hari Krishan, S., Prabhakar, Y., Chowdary, G.V., Ayyanna, C., Simultaneous saccharification and fermentation of pretreated sugar cane leaves to ethanol (1998) Process Biochem, 33, pp. 825-830Lesoing, G., Klopfenstein, T., Rush, I., Ward, J., Chemical treatment of wheat straw (1981) J Anim Sci, 51, pp. 263-269Verma, M.L., Practical aspects of treatment of crop residues (1983) The utilization of fibrous agricultural residues, pp. 85-99. , Australian Govt Punbl Ser, Canberra, Watson Ferguson and Co, G.R. Pearce (Ed.)Playne, M.J., Increased digestability of bagasse by pretreatment with alkali and steam explosion (1984) Biotechnol Bioeng, 26, pp. 426-433Nagwani, M., Calcium hydroxide pretreatment of biomass. (1992), M.S. thesis, Texas A&M University;Chang, V.S., Burr, B., Holtzapple, M.T., Lime pretreatment of switchgrass (1997) Appl Biochem Biotechnol, 63-65, pp. 3-19Chang, V.S., Nagwani, M., Holtzapple, M.T., Lime pretreatment of crop residues bagasse and wheat straw (1998) Appl Biochem Biotechnol, 74, pp. 135-159Holtzapple, M.T., Davison, R.R., Methods of biomass pretreatment. (1999), U.S. Patent number 5,865,898Kim, S., Holtzapple, M.T., Lime pretreatment and enzymatic hydrolysis of corn stover (2005) Bioresour Technol, 96 (18), pp. 1994-2006Szczodrak, J., Fiedurek, J., Technology for conversion of lignocellulosic biomass to ethanol (1996) Biomass Bioenergy, 10 (5-6), pp. 367-375Ghose, T.K., Measurement of cellulase activities (1987) Pure Appl Chem, 59, pp. 257-268Adney, B., Baker, J., (1996) Measurement of cellulase activities: laboratory analytical procedure (LAP), p. 11. , National Renewable Energy Laboratory, Golden (CO),Technical Report NREL/TP-510-42628Wood, T.M., Bhat, K.M., Methods for measuring cellulase activities (1988) Methods in enzymology, 160, pp. 87-111. , Academic Press, New York (NY), W.A. Wood, S.T. Kellog (Eds.) Biomass part A cellulose and hemicelluloseFerraz, A., Baeza, J., Rodriguez, J., Freer, J., Estimating the chemical composition of biodegraded pine and eucalyptus wood by DRIFT spectroscopy and multivariate analysis (2000) Bioresour Technol, 74 (3), pp. 201-212Lin, Y.L., Dence, C.W., (1992) Methods in lignin chemistry, , Springer-Verlag Berlin, New YorkIrick, T.J., West, K., Brownell, H.H., Schiwald, W., Saddler, J.N., Comparison of colorimetric and HPLC techniques for quantitating the carbohydrate components of steam-treated wood (1988) Appl Biochem Biotechnol, 17, pp. 137-149Kaar, W.E., Brink, D.L., Summative analysis of nine common North American woods (1991) J Wood Chem Technol, 11 (4), pp. 479-494Kaar, W.E., Gool, L.G., Merriman, M.M., Brink, D.L., The complete analysis of wood polysaccharides using HPLC (1991) J Wood Chem Technol, 11 (4), pp. 447-463Laver, M.L., Wilson, K.P., Determination of carbohydrates in wood pulp products (1993) Tappi J, 76 (6), pp. 155-158Barros Neto, B., Scarmin, I.S., Bruns, R.E., (2003) Como fazer experimentos. Pesquisa e Desenvolvimento na ciência e na indústria, , Editora da UNICAMP, Campinas, SPJuhász, T., Szengyel, Z., Réczey, K., Siika-Aho, M., Viikari, L., Characterization of cellulases and hemicellulases profuced by Trichoderma reesei on various carbon sources (2005) Process Biochem, 40, pp. 3519-3525