Visualizing Inhibition Of Fatty Acid Synthase Through Mass Spectrometric Analysis Of Mitochondria From Melanoma Cells

dc.creatorZecchin K.G.
dc.creatorAlberici L.C.
dc.creatorRiccio M.F.
dc.creatorEberlin M.N.
dc.creatorVercesi A.E.
dc.creatorGraner E.
dc.creatorCatharino R.R.
dc.date2011
dc.date2015-06-30T20:41:37Z
dc.date2015-11-26T14:53:38Z
dc.date2015-06-30T20:41:37Z
dc.date2015-11-26T14:53:38Z
dc.date.accessioned2018-03-28T22:05:33Z
dc.date.available2018-03-28T22:05:33Z
dc.identifier
dc.identifierRapid Communications In Mass Spectrometry. , v. 25, n. 3, p. 449 - 452, 2011.
dc.identifier9514198
dc.identifier10.1002/rcm.4875
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-78651290740&partnerID=40&md5=048ff1b52f3b21316cfe297e58f655d2
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/108907
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/108907
dc.identifier2-s2.0-78651290740
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1254996
dc.descriptionFatty acid synthase (FASN) is the metabolic enzyme responsible for the endogenous synthesis of the saturated long-chain fatty acid palmitate. In contrast to most normal cells, FASN is overexpressed in a variety of human cancers including cutaneous melanoma, in which its levels of expression are associated with a poor prognosis and depth of invasion. Recently, we have demonstrated the mitochondrial involvement in FASN inhibition-induced apoptosis in melanoma cells. Herein we compare, via electrospray ionization mass spectrometry (ESI-MS), free fatty acids (FFA) composition of mitochondria isolated from control (EtOH-treated cells) and Orlistat-treated B16-F10 mouse melanoma cells. Principal component analysis (PCA) was applied to the ESI-MS data and found to separate the two groups of samples. Mitochondria from control cells showed predominance of six ions, that is, those of m/z 157 (Pelargonic, 9:0), 255 (Palmitic, 16:0), 281 (Oleic, 18:1), 311 (Arachidic, 20:0), 327 (Docosahexaenoic, 22:6) and 339 (Behenic, 22:0). In contrast, FASN inhibition with Orlistat changes significantly mitochondrial FFA composition by reducing synthesis of palmitic acid, and its elongation and unsaturation products, such as arachidic and behenic acids, and oleic acid, respectively. ESI-MS of mitochondria isolated from Orlistat-treated cells presented therefore three major ions of m/z 157 (Pelargonic, 9:0), 193 (unknown) and 199 (Lauric, 12:0). These findings demonstrate therefore that FASN inhibition by Orlistat induces significant changes in the FFA composition of mitochondria. © 2011 John Wiley & Sons, Ltd.
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dc.languageen
dc.publisher
dc.relationRapid Communications in Mass Spectrometry
dc.rightsfechado
dc.sourceScopus
dc.titleVisualizing Inhibition Of Fatty Acid Synthase Through Mass Spectrometric Analysis Of Mitochondria From Melanoma Cells
dc.typeArtículos de revistas


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