Effects Of Plasmodium Berghei On Thymus: High Levels Of Apoptosis And Premature Egress Of Cd4+cd8+ Thymocytes In Experimentally Infected Mice

dc.creatorFrancelin C.
dc.creatorPaulino L.C.
dc.creatorGameiro J.
dc.creatorVerinaud L.
dc.date2011
dc.date2015-06-30T20:37:51Z
dc.date2015-11-26T14:52:13Z
dc.date2015-06-30T20:37:51Z
dc.date2015-11-26T14:52:13Z
dc.date.accessioned2018-03-28T22:04:17Z
dc.date.available2018-03-28T22:04:17Z
dc.identifier
dc.identifierImmunobiology. , v. 216, n. 10, p. 1148 - 1154, 2011.
dc.identifier1712985
dc.identifier10.1016/j.imbio.2011.03.009
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-80052099025&partnerID=40&md5=1fbc8c5f55bf5f1053a2ea3fa2d35854
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/108700
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/108700
dc.identifier2-s2.0-80052099025
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1254778
dc.descriptionWe have previously showed alterations in the thymus during experimental infection with Plasmodium berghei, the causative agent of Malaria. Such alterations comprised histological changes with loss of delimitation between cortical and medullar regions, a profound atrophy with depletion of CD4 +CD8 + double-positive (DP) thymocytes, and severe changes in the expression of cell migration-related molecules, belonging to the extracellular matrix and chemokine protein families. Taken together, these considerations prompted us to evaluate if the acute thymic atrophy observed during Plasmodium infection was correlated with increased apoptotic levels of thymocytes or with their premature emigration to the periphery. Our results confirmed that the marked reduction of the thymus weight in infected animals was accompanied by histological alterations, which included a very large number of cells showing nuclear condensation and karyorrhectic changes surrounded by histiocytes suggesting increased levels of apoptosis. This was confirmed by immunohistochemistry and flow cytometry techniques. In order to verify if an accelerated emigration of thymic cells to the peripheral lymphoid organs was also occurring we analyzed the spleen and mesenteric lymph nodes from control and infected mice. No significant differences were found in the spleen, but were seen after 14 days of infection between control and infected mice in the mesenteric lymph nodes. The main alteration was the presence of double negative (CD4 -CD8 -) and double positive (CD4 +CD8 +) cells. We concluded that both apoptosis of thymocytes and premature egress of immature cells take place during infection. Additional studies will be necessary to verify how such alterations might influence the systemic immune response to the parasite. © 2011 Elsevier GmbH.
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dc.languageen
dc.publisher
dc.relationImmunobiology
dc.rightsfechado
dc.sourceScopus
dc.titleEffects Of Plasmodium Berghei On Thymus: High Levels Of Apoptosis And Premature Egress Of Cd4+cd8+ Thymocytes In Experimentally Infected Mice
dc.typeArtículos de revistas


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