Thermal Stability Of The Immobilized Fructosyltransferase From Rhodotorula Sp

dc.creatorAguiar-Oliveira E.
dc.creatorMaugeri F.
dc.date2011
dc.date2015-06-30T20:37:11Z
dc.date2015-11-26T14:52:04Z
dc.date2015-06-30T20:37:11Z
dc.date2015-11-26T14:52:04Z
dc.date.accessioned2018-03-28T22:04:02Z
dc.date.available2018-03-28T22:04:02Z
dc.identifier
dc.identifierBrazilian Journal Of Chemical Engineering. , v. 28, n. 3, p. 363 - 372, 2011.
dc.identifier1046632
dc.identifier10.1590/S0104-66322011000300002
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-80052989429&partnerID=40&md5=30d1d94be3520dcd16b8d6e8ff58359a
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/108652
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/108652
dc.identifier2-s2.0-80052989429
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1254718
dc.descriptionThe thermal stability of the extracellular fructosyltransferase (FTase) from Rhodotorula sp., recovered from cultivation medium by ethanol precipitation and immobilized onto niobium ore, was studied by Arrhenius plot, half-life profile, half-inactivation temperature (T 50) and thermodynamic parameters. The Arrhenius plot showed two different behaviors with different deactivation energies (E ad) only after immobilization, the transition occurring in the temperature interval between 51 and 52°C. T 50 for the free enzyme was estimated to be around 62°C and, after immobilization, 66°C. After 15 minutes at 52°C, it was also possible to observe enzymatic activation for both the free and immobilized forms, but greater activation was achieved at pH 4.5 with the immobilized enzyme. Between 47-51°C the immobilized enzyme was more stable than the free enzyme, with pH 6.0 being the more stable condition for the immobilized enzyme. However, above 52°C the free form was more stable.
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dc.description3
dc.description363
dc.description372
dc.descriptionAguiar-Oliveira, E., Maugeri, F., Characterization of the immobilized fructosyltranferase from Rhodotorula sp (2010) International Journal of Food Engineering, 6 (3). , DOI 10.2202/1556-3758.1894
dc.descriptionAlvarado-Huallanco, M.B., Maugeri-Filho, F., Kinetics and modeling of fructooligosaccharide synthesis by immobilized fructosyltransferase from Rhodotorula sp (2010) Journal of Chemical Technology & Biotechnology, , DOI 10.1002/jctb.2477
dc.descriptionBecktel, W., Schellman, J.A., Protein stability curves (1987) Biopolymers., 26, pp. 1859-1877
dc.descriptionBhatti, H.N., Rashid, M.H., Asgher, M., Nawaz, R., Khalid, A.M., Perveen, R., Chemical modification results in hyperactivation and thermostabilization of Fusarium solani glucoamylase (2007) Canadian Journal of Microbiology, 53 (2), pp. 177-185. , DOI 10.1139/W06-094
dc.descriptionBlanch, H.W., Clark, D.S., (1997) Biochemical Engineering, , Marcel Dekker, New York
dc.descriptionBolivar, J.M., Mateo, C., Rocha-Martin, J., Cava, F., Berenguer, J., Fernadez-Lafuente, R., Guisan, J.M., The adsorption of multimeric enzymes on very lowly activated supports involves more enzyme subunits: Stabilization of a glutamate dehydrogenase from thermus thermophilus by immobilization on heterofunctional supports (2009) Enzyme and Microbial Technology, 44, pp. 139-144
dc.descriptionBromberg, A., Marx, S., Frishman, G., Kinetic study of the thermal inactivation of cholinesterase enzymes immobilized in solid matrices (2008) Biochimica et Biophysica Acta, 1784, pp. 961-996
dc.descriptionCatana, R., Eloy, M., Rocha, J.R., Ferreira, B.S., Cabral, J.M.S., Fernandes, P., Stability evaluation of an immobilized enzyme system for inulin hydrolysis (2007) Food Chemistry, 101, pp. 260-266
dc.descriptionChen, W., Liu, C., Production of ?-fructofuranosidase by Aspergillus japonicus (1996) Enzyme and Microbial Technology, 18 (2), pp. 153-160
dc.descriptionCornish-Bowden, A., (1995) Fundamentals of Enzyme Kinetics, , Portland Press, London
dc.descriptionDoran, P.M., (2002) Bioprocess Engineering Principles, , Academic Press, New York
dc.descriptionDosztanyi, Z., Fiser, A., Simon, I., Stabilization centers in proteins: Identification, characterization and predictions (1997) Journal of Molecular Biology, 272 (4), pp. 597-612. , DOI 10.1006/jmbi.1997.1242
dc.descriptionFogler, H.S., (1999) Elements of Chemical Reaction Engineering, , 3rd Ed., Prentice Hall, New Jersey
dc.descriptionGibson, G.R., Probert, H.M., Loo, J.V., Rastall, R.A., Roberfroid, M.B., Dietary modulation of the human colonic microbiota: Updating the concept of prebiotcs (2004) Nutrition Research Reviews, 17, pp. 259-275
dc.descriptionHernalsteens, S., Maugeri, F., Purification and characterisation of a fructosyltransferase from Rhodotorula sp (2008) Applied Microbiology Biotechnology, 79, pp. 589-596
dc.descriptionHidaka, H., Hirayama, M., Sumi, N., A fructooligosaccharide-producing enzyme from Aspergillus niger ATCC20611 (1998) Agricultural and Biological Chemistry, 52 (5), pp. 1181-1187
dc.descriptionLumry, R., Eyring, H., Conformation changes of proteins (1954) The Journal of Physical Chemistry, 58, pp. 110-120
dc.descriptionLuscher, M., Erdin, C., Sprenger, N., Hochstrasser, U., Boller, T., Wiemken, A., Inulin synthesis by a combination of purified fructosyltransferases from tubers of Helianthus tuberosus (1996) FEBS Letters, 385 (1-2), pp. 39-42. , DOI 10.1016/0014-5793(96)00343-2
dc.descriptionMaugeri, F., Aguiar-Oliveira, E., (2007) Método Para Imobilização de Enzimas Utilizando Suporte Sólido Inorgânico, , University of Campinas (UNICAMP) Patent: PI 070683-1, Brazil Office
dc.descriptionMaugeri, F., Hernalsteens, S., Screening of yeast strains for transfructosylating activity (2007) Journal of Molecular Catalysis B: Enzymatic, 49 (1-4), pp. 43-49. , DOI 10.1016/j.molcatb.2007.08.001, PII S1381117707001580
dc.descriptionRiaz, M., Perveen, R., Javed, M.R., Nadeem, H., Rashid, M.H., Kinetic and thermodynamic properties of novel glucoamylase from Humicola sp (2007) Enzyme and Microbial Technology, 41 (5), pp. 558-564. , DOI 10.1016/j.enzmictec.2007.05.010, PII S0141022907001731
dc.descriptionNemukula, A., Mutanda, T., Wilhemi, B.S., Whiteley, C.G., Response surface methodology: Synthesis of short chain fructooligosaccharides with a fructosyltransferase from Aspergillus aculeatus (2009) Bioresource Technology, 100, pp. 2040-2045
dc.descriptionO'Fagain, C., Enzyme stabilization - Recent experimental progress (2003) Enzyme and Microbial Technology, 33 (2-3), pp. 137-149. , DOI 10.1016/S0141-0229(03)00160-1
dc.descriptionO'Fágáin, C., (1997) Stabilizing Protein Function, , Springer Verlang, Berlin
dc.descriptionPapinutti, L., Dimitriu, P., Forchiassin, F., Stabilization studies of Fomes sclerodermeus laccases (2008) Bioresource Technology, 99 (2), pp. 419-424. , DOI 10.1016/j.biortech.2006.11.061, PII S0960852407000041
dc.descriptionRuth, D.M., Buckley, S.J., O'Connor, B.F., O'Fagain, C., Solvent and thermal stability, and pH kinetics, of proline-specific dipeptidyl peptidase IV-like enzyme from bovine serum (2007) Enzyme and Microbial Technology, 41 (3), pp. 307-311. , DOI 10.1016/j.enzmictec.2007.02.007, PII S0141022907000786
dc.descriptionSadana, A., Enzyme deactivation (1998) Biotechnology Advances, 6, pp. 349-446
dc.descriptionSantos, A.M.P., Oliveira, M.G., Maugeri, F., Modelling thermal stability and activity of free and immobilized enzymes as a novel tool for enzyme reactor design (2007) Bioresource Technology, 98 (16), pp. 3142-3148. , DOI 10.1016/j.biortech.2006.10.035, PII S0960852406006596
dc.descriptionŠikšnis, V., Mozhaev, V.V., Galkantaite, N., Melik-Nubarov, N.S., Denis, G., Martinek, K., Unusual (zig-zag) temperature dependence of the rate constant for irreversible inactivation of hydrophilized enzymes (1990) Collection of Czechoslovak Chemical Communications, 55, pp. 1366-1371
dc.descriptionTanriseven, A., Aslan, Y., Immobilization of Pectinex Ultra SP-L to produce fructooligosaccharides (2005) Enzyme and Microbial Technology, 36 (4), pp. 550-554. , DOI 10.1016/j.enzmictec.2004.12.001, PII S014102290400362X
dc.descriptionTrevan, M.D., (1980) Immobilized Enzymes - An Introduction and Applications in Biotechnology, , John Wiley & Sons, New York
dc.languageen
dc.publisher
dc.relationBrazilian Journal of Chemical Engineering
dc.rightsaberto
dc.sourceScopus
dc.titleThermal Stability Of The Immobilized Fructosyltransferase From Rhodotorula Sp
dc.typeArtículos de revistas


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