Development And Characterization Of A Cationic Lipid Nanocarrier As Non-viral Vector For Gene Therapy

dc.creatorSeverino P.
dc.creatorSzymanski M.
dc.creatorFavaro M.
dc.creatorAzzoni A.R.
dc.creatorChaud M.V.
dc.creatorSantana M.H.A.
dc.creatorSilva A.M.
dc.creatorSouto E.B.
dc.date2015
dc.date2015-06-25T12:52:05Z
dc.date2015-11-26T14:51:42Z
dc.date2015-06-25T12:52:05Z
dc.date2015-11-26T14:51:42Z
dc.date.accessioned2018-03-28T22:03:27Z
dc.date.available2018-03-28T22:03:27Z
dc.identifier
dc.identifierEuropean Journal Of Pharmaceutical Sciences. Elsevier, v. 66, n. , p. 78 - 82, 2015.
dc.identifier9280987
dc.identifier10.1016/j.ejps.2014.09.021
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-84908426106&partnerID=40&md5=777d92f8317e46d43e9a0904cb01a69c
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/85334
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/85334
dc.identifier2-s2.0-84908426106
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1254571
dc.descriptionThe aim of the present work was to produce a cationic solid lipid nanoparticle (SLN) as non-viral vector for protein delivery. Cationic SLN were produced by double emulsion method, composed of softisan® 100, cetyltrimethylammonium bromide (CTAB), Tween® 80, Span® 80, glycerol and lipoid® S75 loading insulin as model protein. The formulation was characterized in terms of mean hydrodynamic diameter (z-ave), polydispersity index (PI), zeta potential (ZP), stability during storage time, stability after lyophilization, effect of toxicity and transfection ability in HeLa cells, in vitro release profile and morphology. SLN were stable for 30 days and showed minimal changes in their physicochemical properties after lyophilization. The particles exhibited a relatively slow release, spherical morphology and were able to transfect HeLa cells, but toxicity remained an obstacle. Results suggest that SLN are nevertheless promising for delivery of proteins or nucleic acids for gene therapy.
dc.description66
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dc.description78
dc.description82
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dc.languageen
dc.publisherElsevier
dc.relationEuropean Journal of Pharmaceutical Sciences
dc.rightsfechado
dc.sourceScopus
dc.titleDevelopment And Characterization Of A Cationic Lipid Nanocarrier As Non-viral Vector For Gene Therapy
dc.typeArtículos de revistas


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