This study evaluated the pain intensity and behavior during labor and delivery, among women who had natural childbirth, without analgesia, in one of the following positions: semi-sitting, lying on the left side, and lithotomy. This is a descriptive, cross-sectional and correlational study. The instruments used were a validated questionnaire that evaluates pain and behavior during labor and childbirth, from the women's perspective, and two pain scales: one analog and one alphanumerical. It was observed that the pain among women who had childbirth in a lithotomy position was significantly smaller compared to those lying on the left side (p=0.003), though women chose the positions. There was an association between pain and behavior. Pain in labor and childbirth were associated among each other, as was the behavior in these two moments. The results point at an association between the position at childbirth and pain, but it was not possible to identify factors to explain this association; therefore, there is a need for longitudinal studies.453557565Lowe, N.K., The nature of labor pain (2002) Am J Obstet Ginecol, 186 (5), pp. 16-24Niven, C.A., Murphy-Black RM. Memory for labor pain: a review of the literature (2000) Birth, 27 (4), pp. 244-253Souza, F.A.E.F., Pereira, L.V., Giuntini, P.B., Mensuração da dor (2003) Dor: contexto interdisciplinar. Curitiba, pp. 179-186. , Teixeira MJ, editor.Ed. MaioSakata, R.K., Hisatugo, M.K.I., Aoki, S.S., Vlainich, R., Issy, A.M., (2003) Avaliação da dor, pp. 85-93. , Cavalcanti IL, Maddalena ML. Dor. Rio de Janeiro: Sociedade de Anestesiologia do Estado do Rio de JaneiroTornquist, C.S., Paradoxos da humanização em uma maternidade no Brasil (2003) Cad Saúde Pública, 19 (2), pp. 419-427Teixeira, N.Z.F., Pereira, W.R., Parto hospitalar: experiências de mulheres da periferia de Cuiabá-MT (2006) Rev Bras Enferm, 59 (6), pp. 740-744Edianez, G.M., Brüggemann, O.M., Puerperal women's perceptions on vertical and horizontal deliveries (2009) Rev Lat Am Enferm, 17 (2), pp. 153-159Ragnar, I., Altman, D., Tydén, T., Olsson, S.E., Comparison of the maternal experience and duration of labour in two upright delivery positions: a randomized controlled trial (2006) BJOG, 113 (2), pp. 165-170(2005) Riscos e benefícios maternos e neonatais das posições lateral-esquerda e vertical semi-sentada no parto [tese, doutorado], , Basile, ALO., São Paulo: Universidade Federal de São PauloPocock, S.J., (1987) Clinical trials: a pratical approach, , 5 th ed.Chichester: Wiley &ampSonsSabatino, H., Vilarino, J.F., (2000) Avaliação obstétrica de partos em posição de cócoras, pp. 171-185. , Sabatino H. Parto humanizado Campinas: Ed. UNICAMPPolit, D.F., Beck, C.T., The content validity index: are you sure you know what's being reported? (2006) Critique and recommendations Res Nurs Health, 29 (5), pp. 489-497Mamede, F.V., Almeida, A.M., Souza, L., Mamede, M.V., A dor durante o trabalho de parto: o efeito da deambulação (2007) Rev Lat Am Enferm, 15 (6), pp. 1157-1162Hofmeyr, G.F., Gülmezoglu, A.M., Nikodem, V.C., Van, D.S., Hendricks, M.S., Labor experience and b-endorphin levels (1995) Int J Gynecol Obstetr, 50 (3), pp. 299-300Florido, C., Oltras, C.M., Fajardo, M.C., González-Scañuela, E., Villaverde, C., González-Gómez, F., Plasma concentrations of bendorphin and adrenocorticotropic hormone in women with and without childbirth preparation (1997) Eur J Obstet Gynecol Reprod Biol, 73 (2), pp. 121-125Almeida, N.A.M., Silveira, N.A., Bachion, M.M., Sousa, J.T., Concentração plasmática do hormônio adrenocorticotrófico de parturientes submetidas a método não farmacológico de alívio da ansiedade e dor do parto (2005) Rev Lat Am Enferm, 13 (2), pp. 223-228Davim, R.M.B., Torres, G.V., Dantas, J.C., Efetividade de estratégias não farmacológicas no alívio da dor de parturientes no trabalho de parto (2009) Rev Esc Enferm USP, 43 (2), pp. 438-445Leventhal, E.A., Leventhal, H., Shacham, S., Easterling, D.V., Active coping reduces reports of pain from childbirth (1989) J Consult Clin Psychol, 57 (3), pp. 365-371Perissinotti, D.M.N., Figueiró, J.A.B., (2005) Psicoterapias: indicação, modalidade e tratamento para doentes com dor, pp. 93-104. , Figueiró JAB, Angelotti G, Pimenta CAM. Dor e saúde mental. São Paulo: AtheneuSaito, E., Gualda, D.M.R., Worrying about labor and delivery pain and its manifestation during childbearing process (2002) Proceedings of the 8° Brazilian Nursing Communication Symposium [Internet], , http://www.proceedings.scielo.br/scielo.php?script=sci_arttext&pid=MSC0000000052002000200031&;lng=en&;nrm=van, May 2-3São Paulo, Brazil [cited 2009 May 15]Davim, R.M.B., Torres, G.V., Dantas, J.C., (2008) Representação de parturientes acerca da dor de parto. Rev Eletr Enferm [Internet], 10 (1). , http://www.revistas.ufg.br/index.php/fen/article/view/7685/5459, [citado 2009 maio 15] Disponível emPereira, L.V., Sousa, F.A.E.F., Mensuração e avaliação da dor pósoperatória: uma breve revisão (1998) Rev Lat Am Enferm, 6 (3), pp. 77-84