Standardization Of Hyphal Growth Inhibition Rate As A Means Of Evaluating Microsporum Spp. In Vitro Susceptibility To Terbinafine, Griseofulvin, And Ciclopiroxolamine

dc.creatorBiancalana F.S.C.
dc.creatorLyra L.
dc.creatorMoretti M.L.
dc.creatorKamei K.
dc.creatorSchreiber A.Z.
dc.date2011
dc.date2015-06-30T20:26:49Z
dc.date2015-11-26T14:49:34Z
dc.date2015-06-30T20:26:49Z
dc.date2015-11-26T14:49:34Z
dc.date.accessioned2018-03-28T22:00:37Z
dc.date.available2018-03-28T22:00:37Z
dc.identifier
dc.identifierMycopathologia. , v. 172, n. 4, p. 279 - 285, 2011.
dc.identifier0301486X
dc.identifier10.1007/s11046-011-9433-7
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-80052474581&partnerID=40&md5=a6b6f50f7ee89e669f8878291f74f96d
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/107968
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/107968
dc.identifier2-s2.0-80052474581
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1253944
dc.descriptionReference methods for antifungal susceptibility tests recommend the use of conidia as inoculum. However, some isolates produce few conidia, while the invasive form of filamentous fungi in general is hyphae making susceptibility tests infeaseble. These facts suggest that other than conidia broth dilution method is required for susceptibility tests. The aim of this study was to clarify if the hyphal growth inhibition rate could be used as a method of determining the antifungal susceptibility of genus Microsporum. For this reason, a method which traces hyphal tips automatically and measures their growth rate was standardized for Microsporum spp. Control growth curves and test growth curves obtained by real-time observation of the hyphae groups responses to different concentrations of terbinafine, griseofulvin, and ciclopiroxolamine were used to compare with minimum inhibitory concentrations (MICs) obtained by conidia broth microdilution method. A visible reduction in the growth inhibition rate was observed when hyphal activity was evaluated using the third or fourth serial two-fold dilution below the MIC determined by broth microdilution for terbinafine and ciclopiroxolamine. For griseofulvin, this reduction occurred after the fifth dilution below the MIC. This study highlights the importance of the inoculum type used to determine the in vitro susceptibility of Microsporum strains. We conclude that measurement of hyphal growth inhibition, despite being time consuming, could be a suitable method for evaluating antifungal susceptibility, particularly for fungi as Microsporum spp. that produce a small (or not at all) number of conidia. © 2011 Springer Science+Business Media B.V.
dc.description172
dc.description4
dc.description279
dc.description285
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dc.languageen
dc.publisher
dc.relationMycopathologia
dc.rightsfechado
dc.sourceScopus
dc.titleStandardization Of Hyphal Growth Inhibition Rate As A Means Of Evaluating Microsporum Spp. In Vitro Susceptibility To Terbinafine, Griseofulvin, And Ciclopiroxolamine
dc.typeArtículos de revistas


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