Artículos de revistas
Improvement Of Phytase Activity By A New Saccharomyces Cerevisiae Strain Using Statistical Optimization
Registro en:
Enzyme Research. , v. 2011, n. 1, p. - , 2011.
20900406
10.4061/2011/796394
2-s2.0-84869031296
Autor
Ries E.F.
Alves MacEdo G.
Institución
Resumen
Using statistical optimization, we enhanced the activity of phytase by a new Saccharomyces cerevisiae strain cultured in mineral medium. Concentrations of carbon source and inducer of phytase production were optimized using a 2 2 full factorial CCD and response surface methodology (RSM). Urea was fixed as nitrogen source in culture medium (0.15%, w/v). The culture medium consisting of 2.5% sucrose and 0.5% sodium phytate optimally supported the maximum phytase activity. In addition, we found that culture of the yeast at 35°C with shaking at 150 rpm supports maximum phytase production. The validity of this model was verified by culturing the organisms in flasks on a shaker. Using the optimized media and growth conditions, we obtained a 10-fold improvement in the production of phytase by S. cerevisiae. © 2011 Edi Franciele Ries and Gabriela Alves Macedo. 2011 1
Pandey, A., Szakacs, G., Soccol, C.R., Rodriguez-Leon, J.A., Soccol, V.T., Production, purification and properties of microbial phytases (2001) Bioresource Technology, 77 (3), pp. 203-214. , DOI 10.1016/S0960-8524(00)00139-5, PII S0960852400001395 Vats, P., Banerjee, U.C., Production studies and catalytic properties of phytases (myo-inositolhexakisphosphate phosphohydrolases): An overview (2004) Enzyme and Microbial Technology, 35 (1), pp. 3-14. , DOI 10.1016/j.enzmictec.2004.03.010, PII S0141022904000870 Casey, A., Walsh, G., Identification and characterization of a phytase of potential commercial interest (2004) Journal of Biotechnology, 110 (3), pp. 313-322. , DOI 10.1016/j.jbiotec.2004.03.001, PII S0168165604001567 Walsh, G.A., Power, R.F., Headon, D.R., Enzymes in the animal-feed industry (1994) Trends in Food Science and Technology, 5 (3), pp. 81-87. , 2-s2.0-0028404159 Howson, S.J., Davis, R.P., Production of phytate-hydrolysing enzyme by some fungi (1983) Enzyme and Microbial Technology, 5 (5), pp. 377-382. , DOI 10.1016/0141-0229(83)90012-1 Konietzny, U., Greiner, R., Molecular and catalytic properties of phytate-degrading enzymes (phytases) (2002) International Journal of Food Science and Technology, 37 (7), pp. 791-812. , DOI 10.1046/j.1365-2621.2002.00617.x Selle, P.H., Ravindran, V., Microbial phytase in poultry nutrition (2007) Animal Feed Science and Technology, 135 (1-2), pp. 1-41. , DOI 10.1016/j.anifeedsci.2006.06.010, PII S0377840106002574 Casey, A., Walsh, G., Purification and characterization of extracellular phytase from Aspergillus niger ATCC 9142 (2003) Bioresource Technology, 86 (2), pp. 183-188. , 2-s2.0-0037209520 10.1016/S0960-8524(02)00145-1 Sunitha, K., Lee, J.-K., Oh, T.-K., Optimization of medium components for phytase production by E. coli using response surface methodology (1999) Bioprocess and Biosystems Engineering, 21 (6), pp. 477-481 Vohra, A., Satyanarayana, T., Statistical optimization of the medium components by response surface methodology to enhance phytase production by Pichia anomala (2002) Process Biochemistry, 37 (9), pp. 999-1004. , DOI 10.1016/S0032-9592(01)00308-9, PII S0032959201003089 Bogar, B., Szakacs, G., Linden, J.C., Pandey, A., Tengerdy, R.P., Optimization of phytase production by solid substrate fermentation (2003) Journal of Industrial Microbiology and Biotechnology, 30 (3), pp. 183-189 Chadha, B.S., Harmeet, G., Mandeep, M., Saini, H.S., Singh, N., Phytase production by the thermophilic fungus Rhizomucor pusillus (2004) World Journal of Microbiology and Biotechnology, 20 (1), pp. 105-109. , DOI 10.1023/B:WIBI.0000013319.13348.0a Kaur, P., Satyanarayana, T., Production of cell-bound phytase by Pichia anomala in an economical cane molasses medium: Optimization using statistical tools (2005) Process Biochemistry, 40 (9), pp. 3095-3102. , DOI 10.1016/j.procbio.2005.03.059, PII S1359511305001169 Singh, B., Satyanarayana, T., A marked enhancement in phytase production by a thermophilic mould Sporotrichum thermophile using statistical designs in a cost-effective cane molasses medium (2006) Journal of Applied Microbiology, 101 (2), pp. 344-352. , DOI 10.1111/j.1365-2672.2006.02921.x Singh, B., Satyanarayana, T., Improved phytase production by a thermophilic mould Sporotrichum thermophile in submerged fermentation due to statistical optimization (2008) Bioresource Technology, 99 (4), pp. 824-830. , DOI 10.1016/j.biortech.2007.01.007, PII S0960852407000995 Li, X.Y., Liu, Z.Q., Chi, Z.M., Production of phytase by a marine yeast Kodamaea ohmeri BG3 in an oats medium: Optimization by response surface methodology (2008) Bioresource Technology, 99 (14), pp. 6386-6390. , 2-s2.0-43849087993 10.1016/j.biortech.2007.11.065 Segueilha, L., Lambrechts, C., Boze, H., Moulin, G., Galzy, P., Purification and properties of the phytase from Schwanniomyces castellii (1992) Journal of Fermentation and Bioengineering, 74 (1), pp. 7-11. , 2-s2.0-0026702174 10.1016/0922-338X(92)90259-W Lambrechts, C., Boze, H., Segueilha, L., Moulin, G., Galzy, P., Influence of culture conditions on the biosynthesis of Schwanniomyces castelli phytase (1993) Biotechnology Letters, 15 (4), pp. 399-404 Sano, K., Fukuhara, H., Nakamura, Y., Phytase of the yeast Arxula adeninivorans (1999) Biotechnology Letters, 21 (1), pp. 33-38. , DOI 10.1023/A:1005438121763 Vohra, A., Satyanarayana, T., Phytase production by the yeast, Pichia anomala (2001) Biotechnology Letters, 23 (7), pp. 551-554. , DOI 10.1023/A:1010314114053 Mayer, A.F., Hellmuth, K., Schlieker, H., Lopez-Ulibarri, R., Oertel, S., Dahlems, U., Strasser, A.W.M., Van Loon, A.P.G.M., An expression system matures: A highly efficient and cost-effective process for phytase production by recombinant strains of Hansenula polymorpha (1999) Biotechnology and Bioengineering, 63 (3), pp. 373-381. , DOI 10.1002/(SICI)1097-0290(19990505)63:3<373::AID-BIT14>3.0.CO;2-T Han, Y., Wilson, D.B., Lei, X.G., Expression of an Aspergillus niger phytase gene (phyA) in Saccharomyces cerevisiae (1999) Applied and Environmental Microbiology, 65 (5), pp. 1915-1918. , 2-s2.0-0032923801 Han, Y., Lei, X.G., Role of glycosylation in the functional expression of an Aspergillus niger phytase (phyA) in Pichia pastoris (1999) Archives of Biochemistry and Biophysics, 364 (1), pp. 83-90. , DOI 10.1006/abbi.1999.1115 Stockmann, C., Losen, M., Dahlems, U., Knocke, C., Gellisse, G., Buchs, J., Effect of oxygen supply on passaging, stabilizing and srening of recombinant Hansenula polymorpha production strains in test tube cultures (2003) FEMS Yeast Research, 4, pp. 195-205 Huang, H., Luo, H., Yang, P., Meng, K., Wang, Y., Yuan, T., Bai, Y., Yao, B., A novel phytase with preferable characteristics from Yersinia intermedia (2006) Biochemical and Biophysical Research Communications, 350 (4), pp. 884-889. , DOI 10.1016/j.bbrc.2006.09.118, PII S0006291X06021516 Guo, M.-J., Zhuang, Y.-P., Chu, J., Zhang, S.-L., Xiong, A.-S., Peng, R.-H., Yao, Q.-H., Production and purification of a novel thermostable phytase by Pichia pastoris FPHY34 (2007) Process Biochemistry, 42 (12), pp. 1660-1665. , DOI 10.1016/j.procbio.2007.09.003, PII S1359511307002528 Jung, H.K., Park, C.D., Bae, D.H., Hong, J.H., Isolation of alcohol-tolerant amylolytic Saccharomyces cerevisiae and its application to alcohol fermentation (2008) Food Science and Biotechnology, 17 (6), pp. 1160-1164. , 2-s2.0-61349200277 Kim, S.D., Kim, M.K., Woo, C.J., Rhee, C.H., Lee, S.H., Characterization of psychrophilic yeast in Kimchi (2000) Food Science and Biotechnology, 9, pp. 277-279 Nayini, N.R., Markakis, P., The phytase of yeast (1984) Lebensmittel Wissenschaft & Technologie, 17 (1), pp. 24-26 Jin-In, M., Won-Seo, S., Kim, D.C., Soon-Oh, N., Purification and biochemical properties of an extracellular acid phytase produced by the Saccharomyces cerevisiae CY strain (2009) Process Biochemistry, 44 (1), pp. 122-126. , 2-s2.0-58049215335 10.1016/j.procbio.2008.10.006 Ries, E.F., MacEdo, G.A., Progressive screening of thermostable yeasts for phytase production (2009) Food Science and Biotechnology, 18, pp. 655-660 Shimizu, M., Purification and characterization of Phytase from Bacillus subtilis (natto) N-77 (1992) Bioscince Biotechnology and Biochemistry, 56, pp. 1266-1269 Bogar, B., Szakacs, G., Pandey, A., Abdulhameed, S., Linden, J.C., Tengerdy, R.P., Production of phytase by Mucor racemosus in solid-state fermentation (2003) Biotechnology Progress, 19 (2), pp. 312-319. , DOI 10.1021/bp020126v Roopesh, K., Ramachandran, S., Nampoothiri, K.M., Szakacs, G., Pandey, A., Comparison of phytase production on wheat bran and oilcakes in solid-state fermentation by Mucor racemosus (2006) Bioresource Technology, 97 (3), pp. 506-511. , DOI 10.1016/j.biortech.2005.02.046, PII S0960852405001574