Purification Of Bromelain From Ananas Comosus By Peg/phosphate Atps

dc.creatorFerreira J.F.
dc.creatorBresolin L.R.P.
dc.creatorSilveira E.
dc.creatorTambourgi E.B.
dc.date2011
dc.date2015-06-30T20:23:23Z
dc.date2015-11-26T14:48:52Z
dc.date2015-06-30T20:23:23Z
dc.date2015-11-26T14:48:52Z
dc.date.accessioned2018-03-28T21:59:43Z
dc.date.available2018-03-28T21:59:43Z
dc.identifier
dc.identifierChemical Engineering Transactions. Italian Association Of Chemical Engineering - Aidic, v. 24, n. , p. 931 - 936, 2011.
dc.identifier19749791
dc.identifier10.3303/CET1124156
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-79955514730&partnerID=40&md5=c0911629a53e1c3ac0024935104ed27f
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/107760
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/107760
dc.identifier2-s2.0-79955514730
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1253728
dc.descriptionThe bromelains are proteases from vegetable origin, obtained from several species of Bromeliaceae family, which are found in pineapple's stem and fruit. The bromelains uses are based on its proteolytic activity on food industries and mostly at Brazilian pharmaceutical industry. The bromelains has been documented by its therapeutic effects at all inflammatory conditions, in addition to its attested efficiency of cure of various health problems, such as angina, indigestion and respiratory problems. Various bromelains' purification methods have been studied, particularly bromelain of pineapple stem. Aqueous two-phase system has been applied for several years as a good laboratorial separation technique and it can be used as a pre-purification step. This system has been tested with large success, it is formed by two aqueous phases immiscible or partially miscible within themselves, obtained by the addition of hydrophilic polymers or one hydrophilic polymer and a salt, such as the poly(ethylene glycol) - PEG - and potassium phosphate salt systems. This work studied the enzyme characterization and recovery presented in the pineapple's stem and peel, by aqueous two-phase systems (ATPs) liquid-liquid extraction. Batch assays were performed aiming the enzyme extraction and recuperation, using the partition coefficient as indicator. The phase diagrams from PEG and potassium phosphate salts aqueous two-phase systems (ATPs) were obtained for PEG with molecular weight of 4000 at pH 6.0 to 11.0, at 25 °C. Three different tie-lines were also studied. The pH and tie-line length influence on the enzyme partition coefficient were analyzed. © 2011, AIDIC Servizi S.r.l.
dc.description24
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dc.description931
dc.description936
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dc.languageen
dc.publisherItalian Association of Chemical Engineering - AIDIC
dc.relationChemical Engineering Transactions
dc.rightsfechado
dc.sourceScopus
dc.titlePurification Of Bromelain From Ananas Comosus By Peg/phosphate Atps
dc.typeActas de congresos


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