dc.creator | Oliveira C.Z. | |
dc.creator | Santos-Filho N.A. | |
dc.creator | Menaldo D.L. | |
dc.creator | Boldrini-Franca J. | |
dc.creator | Giglio J.R. | |
dc.creator | Calderon L.A. | |
dc.creator | Stabeli R.G. | |
dc.creator | Rodrigues F.H.S. | |
dc.creator | Tasic L. | |
dc.creator | da Silva S.L. | |
dc.creator | Soares A.M. | |
dc.date | 2011 | |
dc.date | 2015-06-30T20:19:03Z | |
dc.date | 2015-11-26T14:48:01Z | |
dc.date | 2015-06-30T20:19:03Z | |
dc.date | 2015-11-26T14:48:01Z | |
dc.date.accessioned | 2018-03-28T21:58:42Z | |
dc.date.available | 2018-03-28T21:58:42Z | |
dc.identifier | | |
dc.identifier | Current Topics In Medicinal Chemistry. , v. 11, n. 20, p. 2509 - 2519, 2011. | |
dc.identifier | 15680266 | |
dc.identifier | | |
dc.identifier | http://www.scopus.com/inward/record.url?eid=2-s2.0-80054111967&partnerID=40&md5=0aba0f888af1f9bb1d35a131a44a040f | |
dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/107548 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/107548 | |
dc.identifier | 2-s2.0-80054111967 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1253485 | |
dc.description | Phospholipases A 2 (PLA 2s) from snake venoms comprise a group of 14-18 kDa proteins, responsible for several toxic effects induced by the whole venom. Considering this, studies aiming at the search for natural inhibitors of these proteins are very important. The present work had as objectives the isolation and functional/structural characterization of a γ-type phospholipase A 2 inhibitor (PLI) from Bothrops jararacussu snake plasma, named γBjussuMIP. This acidic glycoprotein was isolated in a high purity level through affinity chromatography on CNBr-Sepharose 4B coupled with BthTXII, showing a pI ~ 5.5 and molecular weight of 23,500 for the monomer (determined by SDS-PAGE), and 160,000 for the oligomer (determined by molecular exclusion chromatography on Sephacryl S-200). The interaction between γBjussuMIP (MIP) and Phospholipase A 2 (PLA 2) was confirmed using circular dichroism (CD) and emission fluorescence techniques. The helical content of the 1:1 molar mixture was higher than that calculated for the addition of the spectra of the unbound proteins indicating binding. The emission fluorescence experiments pointed that Trp residues in PLA 2 participate in proteins interaction as blue shift of 4 nm was observed. The γBjussuMIP cDNA, obtained by PCR of the liver of B. jararacussu snake, revealed 543 bp codifying for a mature protein of 181 amino acid residues. Alignment of its amino acid sequence with those of other snake γPLIs showed 89-94% of similarity. γBjussuMIP mainly inhibited the pharmacological properties of Asp49 PLA 2s, such as phospholipase, anticoagulant, myotoxic, edema inducing, cytotoxic, bactericidal and lethal activities. In addition, it showed to be able to supplement Bothrops antivenom, potentiating its antimyotoxic effect. The aspects broached in this work will be able to provide complementary information on possible mechanisms of action, relating structure and function, which could result in a better understanding of the inhibitory effects induced by γBjussuMIP. © 2011 Bentham Science Publishers. | |
dc.description | 11 | |
dc.description | 20 | |
dc.description | 2509 | |
dc.description | 2519 | |
dc.description | Yu, B.Z., Berg, O.G., Jain, M.K., The divalent cation is obligatory for the binding of ligands to the catalytic site of secreted PLA2 (1993) Biochem, 32, pp. 6484-6492 | |
dc.description | Schaloske, R.H., Dennis, E.A., The phospholipase A 2 superfamily and its group numbering system. Biochim. Biophys (2006) Acta, 1761, pp. 1246-1259 | |
dc.description | Ownby, C.L., de Selistre, A.H.S., White, S.P., Fletcher, J.E., Lysine 49 phospholipase A 2 (1999) Toxicon, 37, pp. 411-435 | |
dc.description | Ownby, C.L., Structure, function and biophysical aspects of the myotoxins from snake venoms (1998) J. Toxicol. Toxin., 17, pp. 213-238 | |
dc.description | Gutiérrez, J.M., Lomonte, B., Phospholipase A 2 myotoxins from Bothrops snake venoms Venom Phospholipase A 2 enzymes: Structure, function and mechanism, 1997, pp. 321-352. , Kini, R.M., John Wiley & Sons. Chichester | |
dc.description | Pungercar, J., Krizaj, I., Understanding the molecular mechanism underlying the presynaptic toxicity of secreted phospholipase A 2 (2007) Toxicon, 50, pp. 871-892 | |
dc.description | Santos-Filho, N.A., Silveira, L.B., Oliveira, C.Z., Bernardes, C.P., Menaldo, D.L., Fuly, A.L., Arantes, E.C., Soares, A.M., A new acidic myotoxic, anti-platelet and prostaglandin I 2 inductor phospholipase A 2 isolated from Bothrops moojeni snake venom (2008) Toxicon, 52, pp. 908-917 | |
dc.description | Dunn, R.D., Broady, K.W., Snake inhibitors of phospholipase A 2 enzymes (2001) Biochim. Biophys. Acta., 1533, pp. 29-37 | |
dc.description | Faure, G., Natural inhibitors of toxic phospholipase A 2 (2000) Biochemie, 82, pp. 833-840 | |
dc.description | Fortes-Dias, C.L., Endogenous inhibitors of snake venom phospholipase A 2 in the blood plasma of snakes (2002) Toxicon, 40, pp. 481-484 | |
dc.description | Lizano, S., Domont, G., Perales, J., Natural phospholipase A 2 myotoxin inhibitor proteins from snakes, mammals and plants (2003) Toxicon, 42, pp. 963-977 | |
dc.description | Marcussi, S., Sant'Ana, C.D., Oliveira, C.Z., Rueda, A.Q., Menaldo, D.L., Beleboni, R.O., Stábeli, R.G., Soares, A.M., Snake venom phospholipase A 2 (2007) Curr. Top. Med. Chem., 7, pp. 743-756 | |
dc.description | Oliveira, C.Z., Menaldo, D.L., Marcussi, S., Santos-Filho, N.A., Silveira, L.B., Boldrini-França, J., Rodrigues, V.M., Soares, A.M., An alpha-type phospholipase A 2 inhibitor from Bothrops jararacussu snake plasma: Structural and functional characterization (2008) Biochimie, 90, pp. 1506-1514 | |
dc.description | Nobuhisa, I., Inamasu, S., Nakai, M., Tatsui, A., Mimori, T., Ogawa, T., Shimohigashi, Y., Ohno, M., Characterization and evolution of a gene encoding a Trimeresurus flavoviridis serum protein that inhibits basic phospholipase A 2 isozymes in the snakeś venom (1997) Eur. J. Biochem., 249, pp. 838-845 | |
dc.description | Chippaux, J.P., Snake-bites: Appraisal of the global situation (1998) Bull. World. Health. Organ., 76, pp. 515-524 | |
dc.description | Chippaux, J.P., The development and use of immunotherapy in Africa (1998) Toxicon, 36, pp. 1503-1506 | |
dc.description | Andrião-Escarso, S.H., Soares, A.M., Fontes, M.R.M., Fuly, A.L., Correa, F.M.A., Rosa, J.C., Greene, L.J., Giglio, J.R., Structural and functional characterization of an acidic platelet aggregation inhibitor and hypotensive phospholipase A 2 from Bothrops jararacussu snake venom (2002) Biochem. Pharmacol., 64, pp. 723-732 | |
dc.description | Andrião-Escarso, S.H., Soares, A.M., Rodrigues, V.M., Angulo, Y., Diaz, C., Lomonte, B., Gutiérrez, J.M., Giglio, J.R., Myotoxic phospholipases A 2 in Bothrops snake venoms: Effect of chemical modifications on the enzymatic and pharmacological properties of bothropstoxins from Bothrops jararacussu (2000) Biochimie, 82, pp. 755-763 | |
dc.description | Soares, A.M., Guerra-Sá, R.R., Borja-Oliveira, C.R., Rodrigues, V.M., Rodrigues-Simioni, L., Rodrigues, V., Fontes, M.R.M., Giglio, J.R., Structural and functional characterization of BnSP-7, a Lys49 myotoxic phospholipase A 2 homologue from Bothrops neuwiedi pauloensis venom (2000) Arch. Biochem. Biophys., 378, pp. 201-209 | |
dc.description | Soares, A.M., Mancin, A.C., Cechinni, A.L., Arantes, E.C., França, S.C., Gutiérrez, J.M., Giglio, J.R., Effects of chemical modifications of crotoxin B, the phospholipase A 2 subunit of crotoxin from Crotalus durissus terrificus snake venom, on its enzymatic and pharmacological activities (2001) J. Biochem. Cell. Biol., 33, pp. 877-888 | |
dc.description | Soares, A.M., Rodrigues, V.M., Homsi-Brandeburgo, M.I., Toyama, M.H., Lombardi, F.R., Arni, R.K., Giglio, J.R., A rapid procedure for the isolation of the Lys-49 myotoxin II from Bothrops moojeni (caissaca) venom: Biochemical characterization, crystallization, myotoxic and edematogenic activity (1998) Toxicon, 36, pp. 503-514 | |
dc.description | Soares, A.M., Marcussi, S., Stábeli, R.G., França, S.C., Giglio, J.R., Ward, R.J., Arantes, E.C., Structural and functional analysis of BmjMIP, a phospholipase A 2 myotoxin inhibitor protein from Bothrops moojeni snake plasma. Biochem. Biophys (2003) Res. Commun., 302, pp. 193-200 | |
dc.description | Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685 | |
dc.description | Böhm, G., Muhr, R., Jaenicke, R., Quantitative analysis of protein far UV circular dichroism spectra by neural networks (1992) Protein Eng, 5, pp. 191-195 | |
dc.description | Stábeli, R.G., Amui, S.F., Sant'Ana, C.D., Pires, M.G., Nomizo, A., Monteiro, M.C., Romão, P.R., Soares, A.M., Bothrops moojeni myotoxin-II. A Lys-49-phopholipase A 2 homologue: An example of functional versatility of snake venom proteins (2006) Comp. Biochem. Physiol. C Toxicol. Pharmacol., 142, pp. 371-381 | |
dc.description | Angulo, Y., Nunez, C.E., Lizano, S., Soares, A.M., Lomonte, B., Immunochemical properties of the N-terminal helix of myotoxin II, a lysine-49 phospholipase A 2 from Bothrops asper snake venom (2001) Toxicon, 398, pp. 79-887 | |
dc.description | Ohkura, N., Inoue, S., Ikeda, K., Hayashi, K., Isolation and characterization of a phospholipase A 2 inhibitor from the blood plasma of the Thailand cobra Naja Naja Kaouthia (1994) Biochem. Biothys. Res. Commun., 200, pp. 784-788 | |
dc.description | Ohkura, N., Inoue, S., Ikeda, K., Hayashi, K., Two subunits of a phospholipase A 2 inhibitor from the blood plasma of the Thailand cobra having structural similarity to urokinase-type plasminogen activator receptor and Ly-6 related proteins (1994) Biochem. Biophys. Res. Commun., 204, pp. 1212-1218 | |
dc.description | Nobuhisa, I., Inamasu, S., Nakai, M., Tatsui, A., Mimori, T., Ogawa, Y., Shimohigashi, Y., Ohno, M., Characterization and evolution of a gene encoding a Trimeresurus flavoviridis serum protein that inhibits basic phospholipase A 2 isozymes in the snake's venom (1997) Eur. J. Biochem., 249, pp. 838-845 | |
dc.description | Estevão-Costa, M.I., Rocha, B.C., Mudado, M.A., Redondo, R., Franco, G.R., Fortes-Dias, C.L., (2008) Prospection Structural Analysis and Phylogenetic Relationships of Endogenous Gammaphospholipase A 2 Inhibitors in Brazilian Bothrops Snake, 52, pp. 122-129. , Viperidae, Crotalinae | |
dc.description | So, S., Chijiwa, T., Ikeda, N., Nobuhisa, I., Oda-Ueda, N., Hattori, S., Ohno, M., Identification of the B Subtype of γ-phospholipase A 2 inhibitor from Protobothrops flavoviridis serum and molecular evolution of snake serum phospholipase A 2 inhibitors (2008) J. Mol. Evol., 66, pp. 298-307 | |
dc.description | Shirai, R., Toriba, M., Hayashi, K., Ikeda, K., Inoue, S., Identification and characterization of phospholipase A 2 inhibitors from the serum of the japanese rat snake, Elaphe climacophora (2009) Toxicon, 53, pp. 685-692 | |
dc.description | Thwin, M.M., Satish, R.L., Chan, S.T.F., Gopalakrishnakone, P., Functional site of endogenous phospholipse A 2 inhibitor from Python serum (2002) Eur. J. Biochem., 269, pp. 719-727 | |
dc.description | Lizano, S., Angulo, Y., Lomonte, B., Fox, J.W., Lambeau, G., Lazdunski, M., Gutiérrez, J.M., Two phospholipase A 2 inhibitors from the plasma of Cerrophidion (Bothrops) godmani which selectively inhibit two different group-II phospholipase A 2 myotoxins from its own venom: Isolation, molecular cloning and biological properties (2000) Biochim. J., 346, pp. 631-639 | |
dc.description | Fortes-Dias, C.L., Fonseca, B.C., Kochva, E., Diniz, C.R., Purification and properties of an antivenom factor from the plasma of the South American rattlesnake (Crotalus durissus terrificus) (1991) Toxicon, 29, pp. 997-1008 | |
dc.description | Fortes-Dias, C.L., Lin, Y., Ewell, J., Diniz, C.R., Liu, T.Y., A phospholipase A 2 inhibitor from the plasma of the South American rattlesnake (Crotalus durissus terrificus). Protein structure, genomic structure, and mechanism of action (1994) J. Biol. Chem., 269, pp. 15646-15651 | |
dc.description | Kudo, I., Murakami, M., Phospholipase A 2 enzymes (2002) Prostag. Lipid. Mediat., pp. 68+69+3+58 | |
dc.description | Olivo, R.A., Teixeira, C.F.P., Wallace, J.L., Gutiérrez, J.M., Zamuner, S.R., Role of cyclooxygenases in edema forming activity of Bothrops venoms (2007) Toxicon, 49, pp. 670-677 | |
dc.description | Thwin, M.M., Gopalakrishnakone, P., Kini, R.M., Armugam, A., Jeyaseelan, K., Recombinant antitoxic and anti-inflammatory factor from the nonvenomous snake Python reticulates: Phospholipase A 2 inhibition and venom neutralizing potential (2000) Biochem, 39, pp. 9604-9611 | |
dc.description | Lizano, S., Lomonte, B., Fox, J.W., Gutiérrez, J.M., Biochemical characterization and pharmacological properties of a phospholipase A 2 myotoxin inhibitor from the plasma of the snake Bothrops asper (1997) Biochem. J., 326, pp. 853-859 | |
dc.description | da Silva, S.L., Calgarotto, A., Maso, V., Damico, D., Baldasso, P., Veber, C.L., Villar, J.A.F.P., Marangoni, S., Molecular modeling and inhibition of phospholipase A2 by polyhydroxy phenolic compounds (2009) Eur. J. Med. Chem., 44, pp. 312-321 | |
dc.description | Souza, A.D.L., Rodrigues, F.E., Queiroz, A.Q.L., Pereira, J.O., Calgarotto, A.K., Maso, V., Marangoni, S., da Silva, S.L., Koninginins, new snake venom phospholipase A2 inhibitors from endophytic fungus Trichoderma koningii (2008) Toxicon, 51, pp. 240-250 | |
dc.description | da Silva, S.L., Comar, M., Oliveira, K.M.T., Chaar, J.S., Bezerra, E.M., Calgarotto, A.K., Baldasso, P.A., Marangoni, S., Molecular modelling of the inhibition of enzyme PLA2 from snake venom by dipyrone and 1-phenyl-3-methyl-5-pyrazolone (2008) J. Quant. Chem., 108, pp. 2576-2585 | |
dc.description | Villar, J.A.F.P., Lima, F.T.D., Veber, C.L., Oliveira, A.R.M., Calgarotto, A.K., Marangoni, S., da Silva, S.L., Synthesis and evaluation of nitrostyrene derivatives compounds, new snake venom phospholipase A 2 inhibitors (2008) Toxicon, 51, pp. 1467-1478 | |
dc.language | en | |
dc.publisher | | |
dc.relation | Current Topics in Medicinal Chemistry | |
dc.rights | fechado | |
dc.source | Scopus | |
dc.title | Structural And Functional Characterization Of A γ-type Phospholipase A 2 Inhibitor From Bothrops Jararacussu Snake Plasma | |
dc.type | Artículos de revistas | |