dc.creatorOliveira C.Z.
dc.creatorSantos-Filho N.A.
dc.creatorMenaldo D.L.
dc.creatorBoldrini-Franca J.
dc.creatorGiglio J.R.
dc.creatorCalderon L.A.
dc.creatorStabeli R.G.
dc.creatorRodrigues F.H.S.
dc.creatorTasic L.
dc.creatorda Silva S.L.
dc.creatorSoares A.M.
dc.date2011
dc.date2015-06-30T20:19:03Z
dc.date2015-11-26T14:48:01Z
dc.date2015-06-30T20:19:03Z
dc.date2015-11-26T14:48:01Z
dc.date.accessioned2018-03-28T21:58:42Z
dc.date.available2018-03-28T21:58:42Z
dc.identifier
dc.identifierCurrent Topics In Medicinal Chemistry. , v. 11, n. 20, p. 2509 - 2519, 2011.
dc.identifier15680266
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-80054111967&partnerID=40&md5=0aba0f888af1f9bb1d35a131a44a040f
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/107548
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/107548
dc.identifier2-s2.0-80054111967
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1253485
dc.descriptionPhospholipases A 2 (PLA 2s) from snake venoms comprise a group of 14-18 kDa proteins, responsible for several toxic effects induced by the whole venom. Considering this, studies aiming at the search for natural inhibitors of these proteins are very important. The present work had as objectives the isolation and functional/structural characterization of a γ-type phospholipase A 2 inhibitor (PLI) from Bothrops jararacussu snake plasma, named γBjussuMIP. This acidic glycoprotein was isolated in a high purity level through affinity chromatography on CNBr-Sepharose 4B coupled with BthTXII, showing a pI ~ 5.5 and molecular weight of 23,500 for the monomer (determined by SDS-PAGE), and 160,000 for the oligomer (determined by molecular exclusion chromatography on Sephacryl S-200). The interaction between γBjussuMIP (MIP) and Phospholipase A 2 (PLA 2) was confirmed using circular dichroism (CD) and emission fluorescence techniques. The helical content of the 1:1 molar mixture was higher than that calculated for the addition of the spectra of the unbound proteins indicating binding. The emission fluorescence experiments pointed that Trp residues in PLA 2 participate in proteins interaction as blue shift of 4 nm was observed. The γBjussuMIP cDNA, obtained by PCR of the liver of B. jararacussu snake, revealed 543 bp codifying for a mature protein of 181 amino acid residues. Alignment of its amino acid sequence with those of other snake γPLIs showed 89-94% of similarity. γBjussuMIP mainly inhibited the pharmacological properties of Asp49 PLA 2s, such as phospholipase, anticoagulant, myotoxic, edema inducing, cytotoxic, bactericidal and lethal activities. In addition, it showed to be able to supplement Bothrops antivenom, potentiating its antimyotoxic effect. The aspects broached in this work will be able to provide complementary information on possible mechanisms of action, relating structure and function, which could result in a better understanding of the inhibitory effects induced by γBjussuMIP. © 2011 Bentham Science Publishers.
dc.description11
dc.description20
dc.description2509
dc.description2519
dc.descriptionYu, B.Z., Berg, O.G., Jain, M.K., The divalent cation is obligatory for the binding of ligands to the catalytic site of secreted PLA2 (1993) Biochem, 32, pp. 6484-6492
dc.descriptionSchaloske, R.H., Dennis, E.A., The phospholipase A 2 superfamily and its group numbering system. Biochim. Biophys (2006) Acta, 1761, pp. 1246-1259
dc.descriptionOwnby, C.L., de Selistre, A.H.S., White, S.P., Fletcher, J.E., Lysine 49 phospholipase A 2 (1999) Toxicon, 37, pp. 411-435
dc.descriptionOwnby, C.L., Structure, function and biophysical aspects of the myotoxins from snake venoms (1998) J. Toxicol. Toxin., 17, pp. 213-238
dc.descriptionGutiérrez, J.M., Lomonte, B., Phospholipase A 2 myotoxins from Bothrops snake venoms Venom Phospholipase A 2 enzymes: Structure, function and mechanism, 1997, pp. 321-352. , Kini, R.M., John Wiley & Sons. Chichester
dc.descriptionPungercar, J., Krizaj, I., Understanding the molecular mechanism underlying the presynaptic toxicity of secreted phospholipase A 2 (2007) Toxicon, 50, pp. 871-892
dc.descriptionSantos-Filho, N.A., Silveira, L.B., Oliveira, C.Z., Bernardes, C.P., Menaldo, D.L., Fuly, A.L., Arantes, E.C., Soares, A.M., A new acidic myotoxic, anti-platelet and prostaglandin I 2 inductor phospholipase A 2 isolated from Bothrops moojeni snake venom (2008) Toxicon, 52, pp. 908-917
dc.descriptionDunn, R.D., Broady, K.W., Snake inhibitors of phospholipase A 2 enzymes (2001) Biochim. Biophys. Acta., 1533, pp. 29-37
dc.descriptionFaure, G., Natural inhibitors of toxic phospholipase A 2 (2000) Biochemie, 82, pp. 833-840
dc.descriptionFortes-Dias, C.L., Endogenous inhibitors of snake venom phospholipase A 2 in the blood plasma of snakes (2002) Toxicon, 40, pp. 481-484
dc.descriptionLizano, S., Domont, G., Perales, J., Natural phospholipase A 2 myotoxin inhibitor proteins from snakes, mammals and plants (2003) Toxicon, 42, pp. 963-977
dc.descriptionMarcussi, S., Sant'Ana, C.D., Oliveira, C.Z., Rueda, A.Q., Menaldo, D.L., Beleboni, R.O., Stábeli, R.G., Soares, A.M., Snake venom phospholipase A 2 (2007) Curr. Top. Med. Chem., 7, pp. 743-756
dc.descriptionOliveira, C.Z., Menaldo, D.L., Marcussi, S., Santos-Filho, N.A., Silveira, L.B., Boldrini-França, J., Rodrigues, V.M., Soares, A.M., An alpha-type phospholipase A 2 inhibitor from Bothrops jararacussu snake plasma: Structural and functional characterization (2008) Biochimie, 90, pp. 1506-1514
dc.descriptionNobuhisa, I., Inamasu, S., Nakai, M., Tatsui, A., Mimori, T., Ogawa, T., Shimohigashi, Y., Ohno, M., Characterization and evolution of a gene encoding a Trimeresurus flavoviridis serum protein that inhibits basic phospholipase A 2 isozymes in the snakeś venom (1997) Eur. J. Biochem., 249, pp. 838-845
dc.descriptionChippaux, J.P., Snake-bites: Appraisal of the global situation (1998) Bull. World. Health. Organ., 76, pp. 515-524
dc.descriptionChippaux, J.P., The development and use of immunotherapy in Africa (1998) Toxicon, 36, pp. 1503-1506
dc.descriptionAndrião-Escarso, S.H., Soares, A.M., Fontes, M.R.M., Fuly, A.L., Correa, F.M.A., Rosa, J.C., Greene, L.J., Giglio, J.R., Structural and functional characterization of an acidic platelet aggregation inhibitor and hypotensive phospholipase A 2 from Bothrops jararacussu snake venom (2002) Biochem. Pharmacol., 64, pp. 723-732
dc.descriptionAndrião-Escarso, S.H., Soares, A.M., Rodrigues, V.M., Angulo, Y., Diaz, C., Lomonte, B., Gutiérrez, J.M., Giglio, J.R., Myotoxic phospholipases A 2 in Bothrops snake venoms: Effect of chemical modifications on the enzymatic and pharmacological properties of bothropstoxins from Bothrops jararacussu (2000) Biochimie, 82, pp. 755-763
dc.descriptionSoares, A.M., Guerra-Sá, R.R., Borja-Oliveira, C.R., Rodrigues, V.M., Rodrigues-Simioni, L., Rodrigues, V., Fontes, M.R.M., Giglio, J.R., Structural and functional characterization of BnSP-7, a Lys49 myotoxic phospholipase A 2 homologue from Bothrops neuwiedi pauloensis venom (2000) Arch. Biochem. Biophys., 378, pp. 201-209
dc.descriptionSoares, A.M., Mancin, A.C., Cechinni, A.L., Arantes, E.C., França, S.C., Gutiérrez, J.M., Giglio, J.R., Effects of chemical modifications of crotoxin B, the phospholipase A 2 subunit of crotoxin from Crotalus durissus terrificus snake venom, on its enzymatic and pharmacological activities (2001) J. Biochem. Cell. Biol., 33, pp. 877-888
dc.descriptionSoares, A.M., Rodrigues, V.M., Homsi-Brandeburgo, M.I., Toyama, M.H., Lombardi, F.R., Arni, R.K., Giglio, J.R., A rapid procedure for the isolation of the Lys-49 myotoxin II from Bothrops moojeni (caissaca) venom: Biochemical characterization, crystallization, myotoxic and edematogenic activity (1998) Toxicon, 36, pp. 503-514
dc.descriptionSoares, A.M., Marcussi, S., Stábeli, R.G., França, S.C., Giglio, J.R., Ward, R.J., Arantes, E.C., Structural and functional analysis of BmjMIP, a phospholipase A 2 myotoxin inhibitor protein from Bothrops moojeni snake plasma. Biochem. Biophys (2003) Res. Commun., 302, pp. 193-200
dc.descriptionLaemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685
dc.descriptionBöhm, G., Muhr, R., Jaenicke, R., Quantitative analysis of protein far UV circular dichroism spectra by neural networks (1992) Protein Eng, 5, pp. 191-195
dc.descriptionStábeli, R.G., Amui, S.F., Sant'Ana, C.D., Pires, M.G., Nomizo, A., Monteiro, M.C., Romão, P.R., Soares, A.M., Bothrops moojeni myotoxin-II. A Lys-49-phopholipase A 2 homologue: An example of functional versatility of snake venom proteins (2006) Comp. Biochem. Physiol. C Toxicol. Pharmacol., 142, pp. 371-381
dc.descriptionAngulo, Y., Nunez, C.E., Lizano, S., Soares, A.M., Lomonte, B., Immunochemical properties of the N-terminal helix of myotoxin II, a lysine-49 phospholipase A 2 from Bothrops asper snake venom (2001) Toxicon, 398, pp. 79-887
dc.descriptionOhkura, N., Inoue, S., Ikeda, K., Hayashi, K., Isolation and characterization of a phospholipase A 2 inhibitor from the blood plasma of the Thailand cobra Naja Naja Kaouthia (1994) Biochem. Biothys. Res. Commun., 200, pp. 784-788
dc.descriptionOhkura, N., Inoue, S., Ikeda, K., Hayashi, K., Two subunits of a phospholipase A 2 inhibitor from the blood plasma of the Thailand cobra having structural similarity to urokinase-type plasminogen activator receptor and Ly-6 related proteins (1994) Biochem. Biophys. Res. Commun., 204, pp. 1212-1218
dc.descriptionNobuhisa, I., Inamasu, S., Nakai, M., Tatsui, A., Mimori, T., Ogawa, Y., Shimohigashi, Y., Ohno, M., Characterization and evolution of a gene encoding a Trimeresurus flavoviridis serum protein that inhibits basic phospholipase A 2 isozymes in the snake's venom (1997) Eur. J. Biochem., 249, pp. 838-845
dc.descriptionEstevão-Costa, M.I., Rocha, B.C., Mudado, M.A., Redondo, R., Franco, G.R., Fortes-Dias, C.L., (2008) Prospection Structural Analysis and Phylogenetic Relationships of Endogenous Gammaphospholipase A 2 Inhibitors in Brazilian Bothrops Snake, 52, pp. 122-129. , Viperidae, Crotalinae
dc.descriptionSo, S., Chijiwa, T., Ikeda, N., Nobuhisa, I., Oda-Ueda, N., Hattori, S., Ohno, M., Identification of the B Subtype of γ-phospholipase A 2 inhibitor from Protobothrops flavoviridis serum and molecular evolution of snake serum phospholipase A 2 inhibitors (2008) J. Mol. Evol., 66, pp. 298-307
dc.descriptionShirai, R., Toriba, M., Hayashi, K., Ikeda, K., Inoue, S., Identification and characterization of phospholipase A 2 inhibitors from the serum of the japanese rat snake, Elaphe climacophora (2009) Toxicon, 53, pp. 685-692
dc.descriptionThwin, M.M., Satish, R.L., Chan, S.T.F., Gopalakrishnakone, P., Functional site of endogenous phospholipse A 2 inhibitor from Python serum (2002) Eur. J. Biochem., 269, pp. 719-727
dc.descriptionLizano, S., Angulo, Y., Lomonte, B., Fox, J.W., Lambeau, G., Lazdunski, M., Gutiérrez, J.M., Two phospholipase A 2 inhibitors from the plasma of Cerrophidion (Bothrops) godmani which selectively inhibit two different group-II phospholipase A 2 myotoxins from its own venom: Isolation, molecular cloning and biological properties (2000) Biochim. J., 346, pp. 631-639
dc.descriptionFortes-Dias, C.L., Fonseca, B.C., Kochva, E., Diniz, C.R., Purification and properties of an antivenom factor from the plasma of the South American rattlesnake (Crotalus durissus terrificus) (1991) Toxicon, 29, pp. 997-1008
dc.descriptionFortes-Dias, C.L., Lin, Y., Ewell, J., Diniz, C.R., Liu, T.Y., A phospholipase A 2 inhibitor from the plasma of the South American rattlesnake (Crotalus durissus terrificus). Protein structure, genomic structure, and mechanism of action (1994) J. Biol. Chem., 269, pp. 15646-15651
dc.descriptionKudo, I., Murakami, M., Phospholipase A 2 enzymes (2002) Prostag. Lipid. Mediat., pp. 68+69+3+58
dc.descriptionOlivo, R.A., Teixeira, C.F.P., Wallace, J.L., Gutiérrez, J.M., Zamuner, S.R., Role of cyclooxygenases in edema forming activity of Bothrops venoms (2007) Toxicon, 49, pp. 670-677
dc.descriptionThwin, M.M., Gopalakrishnakone, P., Kini, R.M., Armugam, A., Jeyaseelan, K., Recombinant antitoxic and anti-inflammatory factor from the nonvenomous snake Python reticulates: Phospholipase A 2 inhibition and venom neutralizing potential (2000) Biochem, 39, pp. 9604-9611
dc.descriptionLizano, S., Lomonte, B., Fox, J.W., Gutiérrez, J.M., Biochemical characterization and pharmacological properties of a phospholipase A 2 myotoxin inhibitor from the plasma of the snake Bothrops asper (1997) Biochem. J., 326, pp. 853-859
dc.descriptionda Silva, S.L., Calgarotto, A., Maso, V., Damico, D., Baldasso, P., Veber, C.L., Villar, J.A.F.P., Marangoni, S., Molecular modeling and inhibition of phospholipase A2 by polyhydroxy phenolic compounds (2009) Eur. J. Med. Chem., 44, pp. 312-321
dc.descriptionSouza, A.D.L., Rodrigues, F.E., Queiroz, A.Q.L., Pereira, J.O., Calgarotto, A.K., Maso, V., Marangoni, S., da Silva, S.L., Koninginins, new snake venom phospholipase A2 inhibitors from endophytic fungus Trichoderma koningii (2008) Toxicon, 51, pp. 240-250
dc.descriptionda Silva, S.L., Comar, M., Oliveira, K.M.T., Chaar, J.S., Bezerra, E.M., Calgarotto, A.K., Baldasso, P.A., Marangoni, S., Molecular modelling of the inhibition of enzyme PLA2 from snake venom by dipyrone and 1-phenyl-3-methyl-5-pyrazolone (2008) J. Quant. Chem., 108, pp. 2576-2585
dc.descriptionVillar, J.A.F.P., Lima, F.T.D., Veber, C.L., Oliveira, A.R.M., Calgarotto, A.K., Marangoni, S., da Silva, S.L., Synthesis and evaluation of nitrostyrene derivatives compounds, new snake venom phospholipase A 2 inhibitors (2008) Toxicon, 51, pp. 1467-1478
dc.languageen
dc.publisher
dc.relationCurrent Topics in Medicinal Chemistry
dc.rightsfechado
dc.sourceScopus
dc.titleStructural And Functional Characterization Of A γ-type Phospholipase A 2 Inhibitor From Bothrops Jararacussu Snake Plasma
dc.typeArtículos de revistas


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