Histopathological Events And Detection Of Metarhizium Anisopliae Using Specific Primers In Infected Immature Stages Of The Fruit Fly Anastrepha Fraterculus (wiedemann, 1830) (diptera: Tephritidae) [eventos Histológicos E Detecção De Metarhizium Anisopliae Usando Primers Específicos Em Estágios Imaturos Da Mosca Das Frutas Anastrepha Fraterculus (wiedemann, 1830) (diptera: Tephritidae)]

dc.creatorBechara I.J.
dc.creatorDestefano R.H.R.
dc.creatorBresil C.
dc.creatorMessias CL.
dc.date2011
dc.date2015-06-30T20:18:06Z
dc.date2015-11-26T14:47:45Z
dc.date2015-06-30T20:18:06Z
dc.date2015-11-26T14:47:45Z
dc.date.accessioned2018-03-28T21:58:21Z
dc.date.available2018-03-28T21:58:21Z
dc.identifier
dc.identifierBrazilian Journal Of Biology. , v. 71, n. 1, p. 91 - 98, 2011.
dc.identifier15196984
dc.identifier10.1590/S1519-69842011000100014
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-79953201914&partnerID=40&md5=e78671d93d500e7da59b282048a9354a
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/107496
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/107496
dc.identifier2-s2.0-79953201914
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1253404
dc.descriptionThe fungus Metarhizium anisopliae is used on a large scale in Brazil as a microbial control agent against the sugar cane spittlebugs, Mahanarva posticata and M. fimbriolata (Hemiptera., Cercopidae). We applied strain E9 of M. anisopliae in a bioassay on soil, with field doses of conidia to determine if it can cause infection, disease and mortality in immature stages of Anastrepha fraterculus, the South American fruit fly. All the events were studied histologically and at the molecular level during the disease cycle, using a novel histological technique, light green staining, associated with light microscopy, and by PCR, using a specific DNA primer developed for M. anisopliae capable to identify Brazilian strains like E9. The entire infection cycle, which starts by conidial adhesion to the cuticle of the host, followed by germination with or without the formation of an appressorium, penetration through the cuticle and colonisation, with development of a dimorphic phase, hyphal bodies in the hemocoel, and death of the host, lasted 96 hours under the bioassay conditions, similar to what occurs under field conditions. During the disease cycle, the propagules of the entomopathogenic fungus were detected by identifying DNA with the specific primer ITSMet: 5' TCTGAATTTTTTATAAGTAT 3' with ITS4 (5' TCCTCCGCTTATTGATATGC 3') as a reverse primer. This simple methodology permits in situ studies of the infective process, contributing to our understanding of the host-pathogen relationship and allowing monitoring of the efficacy and survival of this entomopathogenic fungus in large-scale applications in the field. It also facilitates monitoring the environmental impact of M. anisopliae on non-target insects.
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dc.languagept
dc.publisher
dc.relationBrazilian Journal of Biology
dc.rightsaberto
dc.sourceScopus
dc.titleHistopathological Events And Detection Of Metarhizium Anisopliae Using Specific Primers In Infected Immature Stages Of The Fruit Fly Anastrepha Fraterculus (wiedemann, 1830) (diptera: Tephritidae) [eventos Histológicos E Detecção De Metarhizium Anisopliae Usando Primers Específicos Em Estágios Imaturos Da Mosca Das Frutas Anastrepha Fraterculus (wiedemann, 1830) (diptera: Tephritidae)]
dc.typeArtículos de revistas


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