Photodynamic Effect Produced By Hene Radiation In Harderian Glands Of Wistar Rats: An Experimental Model For Pdt Studies

dc.creatordos Reis Edmyr R.
dc.creatorNicola Ester M.D.
dc.creatorMetze Konradin
dc.creatorNicola Jorge H.
dc.date2000
dc.date2015-06-30T19:51:41Z
dc.date2015-11-26T14:47:26Z
dc.date2015-06-30T19:51:41Z
dc.date2015-11-26T14:47:26Z
dc.date.accessioned2018-03-28T21:57:52Z
dc.date.available2018-03-28T21:57:52Z
dc.identifier
dc.identifierProceedings Of Spie - The International Society For Optical Engineering. Society Of Photo-optical Instrumentation Engineers, Bellingham, Wa, United States, v. 3914, n. , p. 40 - 45, 2000.
dc.identifier0277786X
dc.identifier
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-0033700891&partnerID=40&md5=f0f51d00d7d8e763f2ecf9cca4a1e8c9
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/107344
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/107344
dc.identifier2-s2.0-0033700891
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1253277
dc.descriptionIn rats, the Harderian Gland secret Protoporphirin IX which is retained at acinar lumina. Since this photosensitizer is important for PDT of malignant tumors, we propose to study this gland as a model to help understanding PDT with endogenous photosensitizers. Twenty Wistar SPF adult rats were submitted to surgical exposure of both Harderian glands, revealing red fluorescence upon UV, characterizing the protoporphirin IX presence. After that, one gland of each pair (one kept as control) was irradiated with an 8mW HeNe (6328 angstron) for 45 minutes, delivering about 2.7 joules/mm 2. After 24 hours a group of 10 animals were sacrificed and the glands removed for histological analysis. The remaining animals were subjected to the same procedure but the glands were removed immediately after laser treatment. Histological and fluorescence analysis immediately after laser irradiation showed cell fragmentation with loss of acinar architecture with diffusion of protoporphirin in the cytoplasm of damaged cells, as well as interstitial edema. After 24 hours these alterations were more pronounced with accentuated loss of intraluminal protoporphirin and beginning of leukocytic demarcation of necrotic areas. The innate Harderian glands of rats, exposed to HeNe laser, showed a similar behavior as tumor tissue under PDT.
dc.description3914
dc.description
dc.description40
dc.description45
dc.languageen
dc.publisherSociety of Photo-Optical Instrumentation Engineers, Bellingham, WA, United States
dc.relationProceedings of SPIE - The International Society for Optical Engineering
dc.rightsaberto
dc.sourceScopus
dc.titlePhotodynamic Effect Produced By Hene Radiation In Harderian Glands Of Wistar Rats: An Experimental Model For Pdt Studies
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución