Artículos de revistas
Bile And Liver Metallothionein Behavior In Copper-exposed Fish
Registro en:
Journal Of Trace Elements In Medicine And Biology. , v. 28, n. 1, p. 70 - 74, 2014.
0946672X
10.1016/j.jtemb.2013.09.003
2-s2.0-84890885951
Autor
Hauser-Davis R.A.
Bastos F.F.
Tuton B.
Chavez Rocha R.
Pierre T.S.
Ziolli R.L.
Arruda M.A.Z.
Institución
Resumen
The present study analyzed metallothionein (MT) excretion from liver to bile in Nile Tilapia (Oreochromis niloticus) exposed to sub-lethal copper concentrations (2mgL-1) in a laboratory setting. MTs in liver and bile were quantified by spectrophotometry after thermal incubation and MT metal-binding profiles were characterized by size exclusion high performance liquid chromatography coupled to ICP-MS (SEC-HPLC-ICP-MS). Results show that liver MT is present in approximately 250-fold higher concentrations than bile MT in non-exposed fish. Differences between the MT profiles from the control and exposed group were observed for both matrices, indicating differential metal-binding behavior when comparing liver and bile MT. This is novel data regarding intra-organ MT comparisons, since differences between organs are usually present only with regard to quantification, not metal-binding behavior. Bile MT showed statistically significant differences between the control and exposed group, while the same did not occur with liver MT. This indicates that MTs synthesized in the liver accumulate more slowly than MTs excreted from liver to bile, since the same fish presented significantly higher MT levels in liver when compared to bile. We postulate that bile, although excreted in the intestine and partially reabsorbed by the same returning to the liver, may also release MT-bound metals more rapidly and efficiently, which may indicate an efficient detoxification route. Thus, we propose that the analysis of bile MTs to observe recent metal exposure may be more adequate than the analysis of liver MTs, since organism responses to metals are more quickly observed in bile, although further studies are necessary. © 2013 Elsevier GmbH. 28 1 70 74 Rotchell, J.M., Clarke, K.R., Newton, L.C., Bird, D.J., Hepatic metallothionein as a biomarker for metal contamination: age effects and seasonal variation in European flounders (Pleuronectes flesus) from the Severn Estuary and Bristol Channel (2001) Mar. Environ. Res., 52, pp. 151-171 Bremner, I., Mehra, R.K., Sato, M., Metallothionein in blood, bile and urine (1987) Experientia Suppl., 52, pp. 507-517 Jaw, S., Jeffery, E.H., Role of metallothionein in biliary metal excretion (1989) J. Toxicol. Environ. Health, 28, pp. 39-51 Mohan, P., Failla, M., Bremner, I., Arthursmith, A., Kerzner, B., Biliary copper excretion in the neonatal rat - role of glutathione and metallothionein (1995) Hepatology, 21, pp. 1051-1057 Hauser-Davis, R.A., Gonçalves, R.A., Ziolli, R.L., de Campos, R.C., A novel report of metallothioneins in fish bile: SDS-PAGE analysis, spectrophotometry quantification and metal speciation characterization by liquid chromatography coupled to ICP-MS (2012) Aquat. Toxicol., 116-117, pp. 54-60 Grosell, M.H., Hogstrand, C., Wood, C.M., Cu uptake and turnover in both Cu-acclimated and non-acclimated rainbow trout (Oncorhynchus mykiss) (1997) Aquat. Toxicol., 38, pp. 257-276 Shah, S.L., Attinag, A., Effects of heavy metals accumulation on the 96-h Lc50 values in Tench (Tinca tinca L.) 1758 (2005) Turk. J. Vet. Anim. Sci., 29, pp. 139-144 Hauser-Davis, R.A., Bastos, F.F., de Oliveira, T.F., Ziolli, R.L., de Campos, R.C., Fish bile as a biomarker for metal exposure (2012) Mar. Pollut. Bull., 64, pp. 1589-1595 Norris, D.O., Camp, J.M., Maldonado, T.A., Woodling, J.D., Some aspects of hepatic function in feral brown trout, Salmo trutta, living in metal contaminated water (2000) Comp. Biochem. Physiol. C Toxicol. Pharmacol., 127, pp. 71-78 Galgani, F., Bocquene, G., Truquet, P., Burgeot, T., Chiffoleau, J.F., Claisse, D., Monitoring of pollutant biochemical effects on marine organisms of the French coasts (1992) Oceanologica Acta, 15, pp. 355-364 McDonald, D.G., Wood, C.M., Branchial mechanisms of acclimation to metals in freshwater fish (1993) Fish Ecophisiol., 9, p. 448 (2011), http://www.inea.rj.gov.br/, INEA. Available at(2003), http://www.mma.gov.br/, CONAMA. Available atRand, G.M., Petrocelli, S.R., (1985) Fundamentals of aquatic toxicology: methods and applications, , Hemisphere, New York, G.M. Rand, S.R. Petrocelli (Eds.) Erk, M., Ivankovi, D., Raspor, B., Pavicic, J., Evaluation of different purification procedures for the electrochemical quantification of mussel metallothioneins (2002) Talanta, 57, pp. 1211-1218 Peterson, G.L., Review of the foline phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall (1979) Anal. Biochem., 100, pp. 201-220 Laemmli, U.K., Cleavage of structural proteins during assembly of head of bacteriophage-T4 (1970) Nature, 227, pp. 680-687 Heukeshoven, J., Dernick, R., Simplified method for silver staining of proteins in polyacrylamide gels and the mechanism of silver staining (1985) Electrophoresis, 6, pp. 103-112 Ellman, G.L., Tissue sulfhydryl groups (1959) Arch. Biochem. Biophys., 82, pp. 70-77 Kagi, J.H.R., Overview of metallothionein (1991) Methods Enzymol., 250, pp. 613-626 Chan, W.K., Devlin, R.H., Functional analysis of sockeye salmon (Oncorhynchus nerka) histone H3, metallothionein B and protamine promoters (1994) Third Asian Fisheries Forum, pp. 626-629 Ghedira, J., Jebali, J., Bouraoui, Z., Banni, M., Guerbej, H., Boussetta, H., Metallothionein and metal levels in liver, gills and kidney of Sparus aurata exposed to sublethal doses of cadmium and copper (2010) Fish Physiol. Biochem., 36, pp. 101-107 Langston, W.J., Chesman, B.S., Burt, G.R., Pope, N.D., McEvoy, J., Metallothionein in liver of eels Anguilla anguilla from the Thames Estuary: an indicator of environmental quality? (2002) Mar. Environ. Res., 53, pp. 263-293 Pathiratne, A., Chandrasekera, L.W.H.U., Pathiratne, K.A.S., Use of biomarkers in Nile tilapia (Oreochromis niloticus) to assess the impacts of pollution in Bolgoda Lake, an urban water body in Sri Lanka (2009) Environ. Monit. Assess., 156, pp. 361-375 Richardson, D.M., Davies, I.M., Moffat, C.F., Pollard, P., Stagg, R.M., Biliary PAH metabolites and EROD activity in flounder (Platichthys flesus) from a contaminated estuarine environment (2001) J. Environ. Monit., 3, pp. 610-615 Viarengo, A., Bettella, E., Fabbri, R., Burlando, B., Lafaurie, M., Heavy metal inhibition of EROD activity in liver microsomes from the bass Dicentrarchus labrax exposed to organic xenobiotics: role of GSH in the reduction of heavy metal effects (1997) Mar. Environ. Res., 44, pp. 1-11 Pedersen, S.N., Lundebye, A.K., Depledge, M.H., Field application of metallothionein and stress protein biomarkers in the shore crab (Carcinus maenas) exposed to trace metals (1997) Aquat. Toxicol., 37, pp. 183-200 Tsangaris, C., Strogyloudi, E., Papathanassiou, E., Measurements of biochemical markers of pollution in mussels Mytilus galloprovincialis from coastal areas of the Saronikos Gulf (Greece) (2004) Mediterr. Mar. Sci., 5, pp. 175-186 Prange, A., Profrock, D., Application of CE-ICP-MS and CE-ESI-MS in metalloproteomics: challenges, developments, and limitations (2005) Anal. Bioanal. Chem., 383, pp. 372-389 Heath, A.G., (1991) Water pollution and fish physiology, p. 359. , Lewis Publishers, Boca Raton, Florida Rodriguez-Cea, A., Arias, A.R.L., de la Campa, M.R., Moreira, J.C., Sanz-Medel, A., Metal speciation of metallothionein in white sea catfish, Netuma barba, and pearl cichlid, Geophagus brasiliensis, by orthogonal liquid chromatography coupled to ICP-MS detection (2006) Talanta, 69, pp. 963-969