Artículos de revistas
Effect Of Crotapotin And Heparin On The Rat Paw Oedema Induced By Different Secretory Phospholipases A2
Registro en:
Toxicon. , v. 38, n. 2, p. 199 - 208, 2000.
410101
10.1016/S0041-0101(99)00143-9
2-s2.0-0033991677
Autor
Landucci E.C.T.
Toyama M.
Marangoni S.
Oliveira B.
Cirino G.
Antunes E.
De Nucci G.
Institución
Resumen
The effects of crotapotin (a non-toxic and non-enzymatic acid polypeptide naturally complexed with phospholipase A2) and heparin on rat paw edema induced by different secretory phospholipases A2 (sPLA2) have been investigated. The ability of crotapotin to affect the enzymatic activity of the sPLA2(s) have also been evaluated. Secretory PLA2(s) obtained from both snake (Naja naja, Naja mocambique mocambique, Crotalus adamanteus and Crotalus durissus terrificus) and bee (Apis mellifera) venoms as well as that from bovine pancreas were used in this study. Rat paw oedema was induced by a single subplantar injection of the sPLA2s (5-30 μg/paw) in absence and presence of either crotapotin (10-100 μg/paw) or heparin (50 U/paw). Paw volume was measured using a hydroplethysmometer. Phospholipase A2 from Naja naja, Naja mocambique mocambique, Apis mellifera venoms and the basic component of Crotalus durissus terrificus venom all induced dose-dependent rat paw oedema whereas those from Crotalus adamanteus venom and bovine pancreas were ineffective. Paw oedema induced by PLA2(s) from both Naja naja and Apis mellifera venoms was significantly (P<0.05) inhibited by crotapotin (0.1-100 μg/site) whereas the Naja mocambique mocambique venom PLA2-induced oedema was significantly potentiated (P<0.05) by this polypeptide (40 μg/site). On the other hand, heparin (50 U/paw) had no effect on the paw oedema induced by PLA2 from Naja naja and Apis mellifera venoms but significantly inhibited the Naja mocambique mocambique venom PLA2-induced oedema. The measurement of the in vitro phospholipasic activity revealed that crotapotin inhibited by 60-70% the enzymatic activities of PLA2(s) from Crotalus adamanteus, Naja mocambique mocambique, Apis mellifera venoms and bovine pancreas. 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