Purification And Characterization Of A Trypsin Inhibitor From Plathymenia Foliolosa Seeds

dc.creatorRamos V.D.S.
dc.creatorSilva G.D.S.
dc.creatorFreire M.D.G.M.
dc.creatorMachado O.L.T.
dc.creatorParra J.R.P.
dc.creatorMacedo M.L.R.
dc.date2008
dc.date2015-06-30T19:37:25Z
dc.date2015-11-26T14:46:08Z
dc.date2015-06-30T19:37:25Z
dc.date2015-11-26T14:46:08Z
dc.date.accessioned2018-03-28T21:55:39Z
dc.date.available2018-03-28T21:55:39Z
dc.identifier
dc.identifierJournal Of Agricultural And Food Chemistry. , v. 56, n. 23, p. 11348 - 11355, 2008.
dc.identifier218561
dc.identifier10.1021/jf802778b
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-58949093617&partnerID=40&md5=a5cff126c28082368db11041705a3406
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/106881
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/106881
dc.identifier2-s2.0-58949093617
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1252718
dc.descriptionA novel trypsin inhibitor (PFTI) was isolated from Plathymenia foliolosa (Benth.) seeds by gel filtration chromatography on a Sephadex G-100, DEAE-Sepharose, and trypsin-Sepharose columns. By SDS-PAGE, PFTI yielded a single band with a Mr of 19 kDa. PFTI inhibited bovine trypsin and bovine chymotrypsin with equilibrium dissociation constants (Ki) of 4 × 10-8 and 1.4 × 10-6 M, respectively. PFTI retained more than 50% of activity at up to 50°C for 30 min, but there were 80 and 100% losses of activity at 60 and 70°C, respectively. DTT affected the activity or stability of PFTI. The N-terminal amino acid sequence of PFTI showed a high degree of homology with various members of the Kunitz family of inhibitors. Anagasta kuehniella is found worldwide; this insect attacks stored grains and products of rice, oat, rye, corn, and wheat. The velvet bean caterpillar (Anticarsia gemmatalis) is considered the main defoliator pest of soybean in Brazil. Diatraea saccharalis, the sugar cane borer, is the major pest of sugar cane crops, and its caterpillar-feeding behavior, inside the stems, hampers control. PFTI showed significant inhibitory activity against trypsin-like proteases present in the larval midguts on A. kuehniella and D. saccharalis and could suppress the growth of larvae. © 2008 American Chemical Society.
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dc.languageen
dc.publisher
dc.relationJournal of Agricultural and Food Chemistry
dc.rightsfechado
dc.sourceScopus
dc.titlePurification And Characterization Of A Trypsin Inhibitor From Plathymenia Foliolosa Seeds
dc.typeArtículos de revistas


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